Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,595	C→T	M15I (ATG→ATA)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,595	0	C	T	100.0%	159.5 / NA	60	M15I (ATG→ATA)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/30);  total (30/30)

GCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGG  >  1/1141352‑1141840                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      accATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGaca                                                                                                                                                                                                                                                <  1:25655/250‑3 (MQ=255)                atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                                                                                                                 <  1:490461/247‑1 (MQ=255)                atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                                                                                                                 <  1:260477/247‑1 (MQ=255)                 tataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGt                                                                                                                                                                                                                                >  1:735545/4‑251 (MQ=255)                           gcgcTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTa                                                                                                                                                                                                                      >  1:655244/1‑251 (MQ=255)                                   ccAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGAtttt                                                                                                                                                                                                              >  1:828561/1‑251 (MQ=255)                                            gCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTGTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGt                                                                                                                                                                                                      <  1:592353/250‑1 (MQ=255)                                                      cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCtt                                                                                                                                                                                               <  1:142079/247‑1 (MQ=255)                                                      cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                                           <  1:725014/251‑1 (MQ=255)                                                      cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                                           <  1:691574/251‑1 (MQ=255)                                                      cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                                           <  1:294045/251‑1 (MQ=255)                                                       aCGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATaca                                                                                                                                                                                          <  1:337627/251‑1 (MQ=255)                                                        cGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                                                                                                                         <  1:58906/251‑1 (MQ=255)                                                          aaTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                                                                                                                            >  1:177883/1‑192 (MQ=255)                                                           aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACaa                                                                                                                                                                                       >  1:342920/1‑250 (MQ=255)                                                           aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAAc                                                                                                                                                                                      <  1:799367/251‑1 (MQ=255)                                                             tccggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACgt                                                                                                                                                                                    <  1:364165/247‑1 (MQ=255)                                                                ggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                                                                                                                            >  1:872881/2‑186 (MQ=255)                                                                   ctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                                                                                                                         >  1:336927/1‑240 (MQ=255)                                                                        aGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATaaa                                                                                                                                                                          <  1:567908/250‑1 (MQ=255)                                                                         ggCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTGGCTTGAACTAATGCCGGTATGGACAAGACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACgt                                                                                                                                                                                    <  1:679189/239‑1 (MQ=255)                                                                                      gCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAAt                                                                                                                                                                         >  1:237594/1‑237 (MQ=255)                                                                                                                  gagatacggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCaataat                                                                                                                               >  1:176362/15‑251 (MQ=255)                                                                                                                       acggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGAt                                                                                                                          <  1:483430/242‑1 (MQ=255)                                                                                                                             tctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTaaga                                                                                                                    <  1:51409/248‑4 (MQ=255)                                                                                                                                  ccattGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCaat                                                                                                                                                              >  1:329920/5‑202 (MQ=255)                                                                                                                                       gttttgcttctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTAAAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGtt                                                                                                          >  1:737734/9‑251 (MQ=255)                                                                                                                                                ctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACt                                                                                                 <  1:644079/251‑1 (MQ=255)                                                                                                                                                  tttGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTgg                                                                                               <  1:598101/251‑1 (MQ=255)                                                                                                                                                          ctgttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGa                                                                                       >  1:728130/26‑251 (MQ=255)                                                                                                                                                          ctgttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGa                                                                                       >  1:135463/26‑251 (MQ=255)                                                                                                                                                            gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGtt                                                                                                          >  1:336742/24‑230 (MQ=255)                                                                                                                                                            gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTgg                                                                                               <  1:133564/218‑1 (MQ=255)                                                                                                                                                            gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGt                                                                                              >  1:84945/24‑242 (MQ=255)                                                                                                                                                            gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAAt                                                                                     >  1:20359/24‑251 (MQ=255)                                                                                                                                                            gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAAt                                                                                     >  1:34751/24‑251 (MQ=255)                                                                                                                                                                    gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACCAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGcc                                                                                                                                              >  1:689314/16‑186 (MQ=255)                                                                                                                                                                    gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAg                                                                                                                                                        >  1:267871/16‑176 (MQ=255)                                                                                                                                                                    gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTg                                                                                                              >  1:430051/16‑218 (MQ=255)                                                                                                                                                                    gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGt                                                                             >  1:580475/16‑251 (MQ=255)                                                                                                                                                                      ttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACgt                                                                                                                                                                                    <  1:594438/133‑1 (MQ=255)                                                                                                                                                                        gtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGtttt                                                                          >  1:773688/12‑250 (MQ=255)                                                                                                                                                                             atactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGc                                                                     >  1:571004/7‑250 (MQ=255)                                                                                                                                                                                  gaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGt                                                                                                           <  1:471331/206‑1 (MQ=255)                                                                                                                                                                                   aTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                                              <  1:655629/251‑1 (MQ=255)                                                                                                                                                                                   aTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                                              <  1:443052/251‑1 (MQ=255)                                                                                                                                                                                                  gcagcagcaccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACttcttcaat                                                                                                                                  >  1:172880/11‑165 (MQ=255)                                                                                                                                                                                                          accATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAg                                       <  1:279456/249‑1 (MQ=255)                                                                                                                                                                                                           ccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                                              <  1:208441/226‑1 (MQ=255)                                                                                                                                                                                                           ccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACtt                                              <  1:262707/242‑1 (MQ=255)                                                                                                                                                                                                            cATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGc                                     <  1:372885/251‑1 (MQ=255)                                                                                                                                                                                                               caactaCCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAgg                                      >  1:280069/1‑247 (MQ=255)                                                                                                                                                                                                                  ctacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGttt                                >  1:149322/3‑250 (MQ=255)                                                                                                                                                                                                                   tacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCg                              <  1:842939/250‑1 (MQ=255)                                                                                                                                                                                                                     ccGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATcc                                                                                                      >  1:874890/1‑177 (MQ=255)                                                                                                                                                                                                                     ccGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAg                            >  1:56737/1‑251 (MQ=255)                                                                                                                                                                                                                           tACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCttt                      >  1:897662/1‑251 (MQ=255)                                                                                                                                                                                                                                ctagCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGc                  <  1:329409/247‑1 (MQ=255)                                                                                                                                                                                                                                  agCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCa                 >  1:72966/2‑249 (MQ=255)                                                                                                                                                                                                                                                ccGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACggg  <  1:564945/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGG  >  1/1141352‑1141840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑