Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,586	C→T	L18L (TTG→TTA)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,586	0	C	T	100.0%	163.8 / NA	61	L18L (TTG→TTA)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

ATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCA  >  1/1141345‑1141825                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                aTTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCggt                                                                                                                                                                                                                                        >  1:687568/1‑250 (MQ=255) aTTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCggt                                                                                                                                                                                                                                        >  1:244693/1‑250 (MQ=255)        accATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGaca                                                                                                                                                                                                                                 <  1:25655/250‑3 (MQ=255)                       atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                                                                                                  <  1:490461/247‑1 (MQ=255)                       atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                                                                                                  <  1:260477/247‑1 (MQ=255)                        tataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGt                                                                                                                                                                                                                 >  1:735545/4‑251 (MQ=255)                                  gcgcTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTa                                                                                                                                                                                                       >  1:655244/1‑251 (MQ=255)                                          ccAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGAtttt                                                                                                                                                                                               >  1:828561/1‑251 (MQ=255)                                                   gCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTGTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGt                                                                                                                                                                                       <  1:592353/250‑1 (MQ=255)                                                             cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCtt                                                                                                                                                                                <  1:142079/247‑1 (MQ=255)                                                             cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                            <  1:725014/251‑1 (MQ=255)                                                             cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                            <  1:691574/251‑1 (MQ=255)                                                             cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                                                                                                            <  1:294045/251‑1 (MQ=255)                                                              aCGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATaca                                                                                                                                                                           <  1:337627/251‑1 (MQ=255)                                                               cGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                                                                                                          <  1:58906/251‑1 (MQ=255)                                                                 aaTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                                                                                                             >  1:177883/1‑192 (MQ=255)                                                                  aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACaa                                                                                                                                                                        >  1:342920/1‑250 (MQ=255)                                                                  aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAAc                                                                                                                                                                       <  1:799367/251‑1 (MQ=255)                                                                    tccggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACgt                                                                                                                                                                     <  1:364165/247‑1 (MQ=255)                                                                       ggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                                                                                                             >  1:872881/2‑186 (MQ=255)                                                                          ctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                                                                                                          >  1:336927/1‑240 (MQ=255)                                                                               aGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATaaa                                                                                                                                                           <  1:567908/250‑1 (MQ=255)                                                                                ggCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTGGCTTGAACTAATGCCGGTATGGACAAGACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACgt                                                                                                                                                                     <  1:679189/239‑1 (MQ=255)                                                                                             gCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAAt                                                                                                                                                          >  1:237594/1‑237 (MQ=255)                                                                                                                         gagatacggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCaataat                                                                                                                >  1:176362/15‑251 (MQ=255)                                                                                                                              acggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGAt                                                                                                           <  1:483430/242‑1 (MQ=255)                                                                                                                                    tctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTaaga                                                                                                     <  1:51409/248‑4 (MQ=255)                                                                                                                                         ccattGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCaat                                                                                                                                               >  1:329920/5‑202 (MQ=255)                                                                                                                                              gttttgcttctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTAAAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGtt                                                                                           >  1:737734/9‑251 (MQ=255)                                                                                                                                                       ctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACt                                                                                  <  1:644079/251‑1 (MQ=255)                                                                                                                                                         tttGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTgg                                                                                <  1:598101/251‑1 (MQ=255)                                                                                                                                                                 ctgttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGa                                                                        >  1:728130/26‑251 (MQ=255)                                                                                                                                                                 ctgttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGa                                                                        >  1:135463/26‑251 (MQ=255)                                                                                                                                                                   gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGtt                                                                                           >  1:336742/24‑230 (MQ=255)                                                                                                                                                                   gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTgg                                                                                <  1:133564/218‑1 (MQ=255)                                                                                                                                                                   gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGt                                                                               >  1:84945/24‑242 (MQ=255)                                                                                                                                                                   gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAAt                                                                      >  1:34751/24‑251 (MQ=255)                                                                                                                                                                   gttgaacagtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAAt                                                                      >  1:20359/24‑251 (MQ=255)                                                                                                                                                                           gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACCAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGcc                                                                                                                               >  1:689314/16‑186 (MQ=255)                                                                                                                                                                           gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAg                                                                                                                                         >  1:267871/16‑176 (MQ=255)                                                                                                                                                                           gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTg                                                                                               >  1:430051/16‑218 (MQ=255)                                                                                                                                                                           gtttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGt                                                              >  1:580475/16‑251 (MQ=255)                                                                                                                                                                             ttgtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACgt                                                                                                                                                                     <  1:594438/133‑1 (MQ=255)                                                                                                                                                                               gtaacatactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGtttt                                                           >  1:773688/12‑250 (MQ=255)                                                                                                                                                                                    atactgaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGc                                                      >  1:571004/7‑250 (MQ=255)                                                                                                                                                                                         gaTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGt                                                                                            <  1:471331/206‑1 (MQ=255)                                                                                                                                                                                          aTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                               <  1:443052/251‑1 (MQ=255)                                                                                                                                                                                          aTTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                               <  1:655629/251‑1 (MQ=255)                                                                                                                                                                                                         gcagcagcaccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACttcttcaat                                                                                                                   >  1:172880/11‑165 (MQ=255)                                                                                                                                                                                                                 accATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAg                        <  1:279456/249‑1 (MQ=255)                                                                                                                                                                                                                  ccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAAc                                               <  1:208441/226‑1 (MQ=255)                                                                                                                                                                                                                  ccATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACtt                               <  1:262707/242‑1 (MQ=255)                                                                                                                                                                                                                   cATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGc                      <  1:372885/251‑1 (MQ=255)                                                                                                                                                                                                                      caactaCCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAgg                       >  1:280069/1‑247 (MQ=255)                                                                                                                                                                                                                         ctacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGttt                 >  1:149322/3‑250 (MQ=255)                                                                                                                                                                                                                          tacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCg               <  1:842939/250‑1 (MQ=255)                                                                                                                                                                                                                            ccGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATcc                                                                                       >  1:874890/1‑177 (MQ=255)                                                                                                                                                                                                                            ccGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAg             >  1:56737/1‑251 (MQ=255)                                                                                                                                                                                                                                  tACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCttt       >  1:897662/1‑251 (MQ=255)                                                                                                                                                                                                                                       ctagCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGc   <  1:329409/247‑1 (MQ=255)                                                                                                                                                                                                                                         agCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCa  >  1:72966/2‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCA  >  1/1141345‑1141825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑