Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,520	A→T	L40L (CTT→TTA)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,520	0	A	T	100.0%	108.9 / NA	42	L40L (CTT→TTA)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/24);  total (18/24)

CGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGG  >  1/1141291‑1141747                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    cgcTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGa                                                                                                                                                                                                                >  1:703525/1‑251 (MQ=255)                           aCTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTCGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCgg                                                                                                                                                                                      <  1:447852/251‑1 (MQ=255)                             tAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGaa                                                                                                                                                                                    <  1:803527/251‑1 (MQ=255)                                aTCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATAc                                                                                                                                                                                 <  1:110855/251‑1 (MQ=255)                                           gggTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTga                                                                                                                                                                      >  1:245680/1‑250 (MQ=255)                                                       aTTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATcaactac                                                                                                                                                                                         >  1:620605/1‑217 (MQ=255)                                                       aTTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCggt                                                                                                                                                          >  1:687568/1‑250 (MQ=255)                                                       aTTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCggt                                                                                                                                                          >  1:244693/1‑250 (MQ=255)                                                              accATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGaca                                                                                                                                                   <  1:25655/250‑3 (MQ=255)                                                                     atttCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATcaact                                                                                                                                                                                           >  1:695142/1‑203 (MQ=255)                                                                            cATATAAACTATGCGCTCCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGcagc                                                                                                                                                                                                     <  1:435093/187‑4 (MQ=255)                                                                             atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                    <  1:490461/247‑1 (MQ=255)                                                                             atataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAg                                                                                                                                    <  1:260477/247‑1 (MQ=255)                                                                              tataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTgaactaatgc                                                                                                                                                              <  1:809715/221‑10 (MQ=255)                                                                              tataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTgaac                                                                                                                                                                    <  1:809530/215‑4 (MQ=255)                                                                              tataaaCTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGt                                                                                                                                   >  1:735545/4‑251 (MQ=255)                                                                                        gcgcTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTa                                                                                                                         >  1:655244/1‑251 (MQ=255)                                                                                                ccAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGAtttt                                                                                                                 >  1:828561/1‑251 (MQ=255)                                                                                                         gCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTGTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGt                                                                                                         <  1:592353/250‑1 (MQ=255)                                                                                                                   cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCtt                                                                                                  <  1:142079/247‑1 (MQ=255)                                                                                                                   cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                              <  1:725014/251‑1 (MQ=255)                                                                                                                   cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                              <  1:294045/251‑1 (MQ=255)                                                                                                                   cACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATac                                                                                              <  1:691574/251‑1 (MQ=255)                                                                                                                    aCGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATaca                                                                                             <  1:337627/251‑1 (MQ=255)                                                                                                                     cGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                            <  1:58906/251‑1 (MQ=255)                                                                                                                       aaTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                               >  1:177883/1‑192 (MQ=255)                                                                                                                        aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACaa                                                                                          >  1:342920/1‑250 (MQ=255)                                                                                                                        aTTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAAc                                                                                         <  1:799367/251‑1 (MQ=255)                                                                                                                          tccggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACgt                                                                                       <  1:364165/247‑1 (MQ=255)                                                                                                                             ggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCt                                                                                                                                                                         >  1:463962/2‑166 (MQ=255)                                                                                                                             ggtctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGacaagac                                                                                                                                               >  1:872881/2‑186 (MQ=255)                                                                                                                                ctgtGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATacac                                                                                            >  1:336927/1‑240 (MQ=255)                                                                                                                                     aGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATaaa                                                                             <  1:567908/250‑1 (MQ=255)                                                                                                                                      ggCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTGGCTTGAACTAATGCCGGTATGGACAAGACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACgt                                                                                       <  1:679189/239‑1 (MQ=255)                                                                                                                                                   gCATACGCCTTGAGTTCGTTTTTGTAATGAGATACGGTTTCTGTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAAt                                                                            >  1:237594/1‑237 (MQ=255)                                                                                                                                                                               gagatacggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCaataat                                  >  1:176362/15‑251 (MQ=255)                                                                                                                                                                                    acggtttctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGAt                             <  1:483430/242‑1 (MQ=255)                                                                                                                                                                                          tctgTCCATTGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTaaga                       <  1:51409/248‑4 (MQ=255)                                                                                                                                                                                               ccattGTTTTGCTTCTTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCaat                                                                 >  1:329920/5‑202 (MQ=255)                                                                                                                                                                                                    gttttgcttctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTAAAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGtt             >  1:737734/9‑251 (MQ=255)                                                                                                                                                                                                             ctTTTGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACt    <  1:644079/251‑1 (MQ=255)                                                                                                                                                                                                               tttGCCCACTGTTGAACAGTTTGTAACATACTGATTTGCCGTTGACCTGCAGCAGCACCATCAACTACCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTgg  <  1:598101/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    CGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAACTGTATCCGTCCACTGTTTAGCCTCTTTTGCCCATTGTTGCACCGTTTGAAGCATGCTCATTTGACGTTGACCGGCAGCTGCGGCATCAACAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGG  >  1/1141291‑1141747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑