Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,241	C→T	K133K (AAG→AAA)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,241	0	C	T	100.0%	192.5 / NA	71	K133K (AAG→AAA)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/32);  total (39/32)

GAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAA  >  1/1141007‑1141470                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      gAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaaa                                                                                                                                                                                                                       >  1:190311/1‑251 (MQ=255)     aaaTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGtt                                                                                                                                                                                                                   >  1:252915/1‑251 (MQ=255)       aTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGt                                                                                                                                                                                                                 >  1:49409/1‑251 (MQ=255)                  cTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAg                                                                                                                                                                                                                      <  1:269481/235‑1 (MQ=255)                    ttttGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAg                                                                                                                                                                                                    >  1:880963/1‑251 (MQ=255)                              gCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCa                                                                                                                                                                                          >  1:827840/1‑251 (MQ=255)                                   gCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcg                                                                                                                                                                                      >  1:405385/1‑250 (MQ=255)                                    cGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcgcg                                                                                                                                                                                    <  1:746470/251‑1 (MQ=255)                                        cAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTa                                                                                                                                                                                >  1:671825/1‑251 (MQ=255)                                           cTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAATGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATg                                                                                                                                                                             >  1:447650/1‑251 (MQ=255)                                                        gCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCAc                                                                                                                                                                 >  1:482867/1‑250 (MQ=255)                                                             ttttAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGaaa                                                                                                                                                           >  1:805680/1‑251 (MQ=255)                                                                   gTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaa                                                                                                                                                                                                                        >  1:523565/1‑184 (MQ=255)                                                                    tCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAt                                                                                                                                                     <  1:58629/250‑1 (MQ=255)                                                                                                        cgtttagcgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                                                >  1:673838/13‑251 (MQ=255)                                                                                                               cgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAcc                                                                                                          <  1:690135/245‑1 (MQ=255)                                                                                                                      atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAGATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:812671/232‑1 (MQ=255)                                                                                                                      atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:299863/232‑1 (MQ=255)                                                                                                                      atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTGGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:426661/232‑1 (MQ=255)                                                                                                                        caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:329674/234‑1 (MQ=255)                                                                                                                        caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:850073/234‑1 (MQ=255)                                                                                                                           tagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                                             <  1:757994/237‑1 (MQ=255)                                                                                                                             gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCg                                                                                                                                                                           <  1:607727/159‑1 (MQ=255)                                                                                                                             gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcg                                                                                            >  1:719511/13‑250 (MQ=255)                                                                                                                                 tcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAc                                                                                                           <  1:96845/223‑1 (MQ=255)                                                                                                                                        acTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:675182/2‑233 (MQ=255)                                                                                                                                         cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTc                                                                                                                                                                                                          >  1:451876/1‑128 (MQ=255)                                                                                                                                         cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                                                <  1:211021/218‑1 (MQ=255)                                                                                                                                            ttGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCATCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGc                                                                            <  1:441807/251‑1 (MQ=255)                                                                                                                                             tGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCt                                                                           <  1:600379/251‑1 (MQ=255)                                                                                                                                                cgttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                                             >  1:487590/3‑230 (MQ=255)                                                                                                                                                  ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      <  1:412725/251‑1 (MQ=255)                                                                                                                                                  ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      <  1:548180/251‑1 (MQ=255)                                                                                                                                                     cTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                                             <  1:263973/209‑1 (MQ=255)                                                                                                                                                       ttCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCAc                                                                 <  1:317149/251‑1 (MQ=255)                                                                                                                                                               aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACTATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACt                                                                                                 >  1:357477/1‑211 (MQ=255)                                                                                                                                                               aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATat                                                                                                      <  1:254252/206‑2 (MQ=255)                                                                                                                                                               aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:34871/212‑1 (MQ=255)                                                                                                                                                               aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTccg                                                         >  1:632917/1‑250 (MQ=255)                                                                                                                                                                    aagaagCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                                             <  1:665406/194‑1 (MQ=255)                                                                                                                                                                        agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCGCCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:325610/1‑251 (MQ=255)                                                                                                                                                                        agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:372749/201‑1 (MQ=255)                                                                                                                                                                        agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:306939/1‑251 (MQ=255)                                                                                                                                                                        agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:603477/1‑251 (MQ=255)                                                                                                                                                                         gCTCATACTCTCCACGGTGGAAACAATATTTGCTTCGCAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAgg                                               >  1:32216/1‑251 (MQ=255)                                                                                                                                                                          cTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      >  1:895558/1‑227 (MQ=255)                                                                                                                                                                            cATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTa                                            >  1:495735/1‑251 (MQ=255)                                                                                                                                                                               actctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:587004/5‑194 (MQ=255)                                                                                                                                                                                ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                                        <  1:350774/248‑1 (MQ=255)                                                                                                                                                                                ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                                        <  1:59247/248‑1 (MQ=255)                                                                                                                                                                                  ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcgc                                                                                           >  1:427095/2‑198 (MQ=255)                                                                                                                                                                                  ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCcggtc                                                      >  1:830797/2‑235 (MQ=255)                                                                                                                                                                                  ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                                      >  1:724952/2‑251 (MQ=255)                                                                                                                                                                                  ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                                      >  1:728930/2‑251 (MQ=255)                                                                                                                                                                                         gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCGCCATATAAAc                                                                                                  <  1:86324/184‑1 (MQ=255)                                                                                                                                                                                         gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTgg                                       >  1:816596/1‑243 (MQ=255)                                                                                                                                                                                         gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATa                               >  1:854013/1‑251 (MQ=255)                                                                                                                                                                                           ggAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACg                             >  1:92052/1‑251 (MQ=255)                                                                                                                                                                                             aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc                           <  1:639592/251‑2 (MQ=255)                                                                                                                                                                                             aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc                           <  1:234735/251‑2 (MQ=255)                                                                                                                                                                                             aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc                            <  1:515667/250‑1 (MQ=255)                                                                                                                                                                                             aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc                            >  1:42943/1‑250 (MQ=255)                                                                                                                                                                                                cAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttg                        >  1:656982/1‑247 (MQ=255)                                                                                                                                                                                                  atatTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttgag                      <  1:358342/251‑7 (MQ=255)                                                                                                                                                                                                           tCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:674913/166‑1 (MQ=255)                                                                                                                                                                                                                agAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCttttggc                                                                                                                                                                                           <  1:679439/71‑4 (MQ=255)                                                                                                                                                                                                                    gtgaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgaga    >  1:142724/4‑248 (MQ=255)                                                                                                                                                                                                                      gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttattt                                                                                                              >  1:509456/2‑143 (MQ=255)                                                                                                                                                                                                                      gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:760279/2‑155 (MQ=255)                                                                                                                                                                                                                      gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgagata  >  1:350373/2‑246 (MQ=255)                                                                                                                                                                                                                      gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgagata  >  1:239945/2‑246 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      GAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAA  >  1/1141007‑1141470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑