Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,235	T→C	L135L (TTA→TTG)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,235	0	T	C	100.0%	203.6 / NA	72	L135L (TTA→TTG)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/32);  total (40/32)

GTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAA  >  1/1140993‑1141470                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            gTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAAttttt                                                                                                                                                                                                                                     >  1:645212/1‑248 (MQ=255)               gAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaaa                                                                                                                                                                                                                       >  1:190311/1‑251 (MQ=255)                   aaaTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGtt                                                                                                                                                                                                                   >  1:252915/1‑251 (MQ=255)                     aTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGt                                                                                                                                                                                                                 >  1:49409/1‑251 (MQ=255)                                cTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAg                                                                                                                                                                                                                      <  1:269481/235‑1 (MQ=255)                                  ttttGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAg                                                                                                                                                                                                    >  1:880963/1‑251 (MQ=255)                                            gCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCa                                                                                                                                                                                          >  1:827840/1‑251 (MQ=255)                                                 gCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcg                                                                                                                                                                                      >  1:405385/1‑250 (MQ=255)                                                  cGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcgcg                                                                                                                                                                                    <  1:746470/251‑1 (MQ=255)                                                      cAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTa                                                                                                                                                                                >  1:671825/1‑251 (MQ=255)                                                         cTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAATGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATg                                                                                                                                                                             >  1:447650/1‑251 (MQ=255)                                                                      gCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCAc                                                                                                                                                                 >  1:482867/1‑250 (MQ=255)                                                                           ttttAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGaaa                                                                                                                                                           >  1:805680/1‑251 (MQ=255)                                                                                 gTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaa                                                                                                                                                                                                                        >  1:523565/1‑184 (MQ=255)                                                                                  tCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAt                                                                                                                                                     <  1:58629/250‑1 (MQ=255)                                                                                                                      cgtttagcgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                                                >  1:673838/13‑251 (MQ=255)                                                                                                                             cgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAcc                                                                                                          <  1:690135/245‑1 (MQ=255)                                                                                                                                    atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAGATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:812671/232‑1 (MQ=255)                                                                                                                                    atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:299863/232‑1 (MQ=255)                                                                                                                                    atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTGGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:426661/232‑1 (MQ=255)                                                                                                                                      caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:329674/234‑1 (MQ=255)                                                                                                                                      caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:850073/234‑1 (MQ=255)                                                                                                                                         tagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                                             <  1:757994/237‑1 (MQ=255)                                                                                                                                           gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCg                                                                                                                                                                           <  1:607727/159‑1 (MQ=255)                                                                                                                                           gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcg                                                                                            >  1:719511/13‑250 (MQ=255)                                                                                                                                               tcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAc                                                                                                           <  1:96845/223‑1 (MQ=255)                                                                                                                                                      acTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:675182/2‑233 (MQ=255)                                                                                                                                                       cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTc                                                                                                                                                                                                          >  1:451876/1‑128 (MQ=255)                                                                                                                                                       cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                                                <  1:211021/218‑1 (MQ=255)                                                                                                                                                          ttGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCATCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGc                                                                            <  1:441807/251‑1 (MQ=255)                                                                                                                                                           tGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCt                                                                           <  1:600379/251‑1 (MQ=255)                                                                                                                                                              cgttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                                             >  1:487590/3‑230 (MQ=255)                                                                                                                                                                ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      <  1:412725/251‑1 (MQ=255)                                                                                                                                                                ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      <  1:548180/251‑1 (MQ=255)                                                                                                                                                                   cTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                                             <  1:263973/209‑1 (MQ=255)                                                                                                                                                                     ttCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCAc                                                                 <  1:317149/251‑1 (MQ=255)                                                                                                                                                                             aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACTATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACt                                                                                                 >  1:357477/1‑211 (MQ=255)                                                                                                                                                                             aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATat                                                                                                      <  1:254252/206‑2 (MQ=255)                                                                                                                                                                             aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                                                <  1:34871/212‑1 (MQ=255)                                                                                                                                                                             aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTccg                                                         >  1:632917/1‑250 (MQ=255)                                                                                                                                                                                  aagaagCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                                             <  1:665406/194‑1 (MQ=255)                                                                                                                                                                                      agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCGCCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:325610/1‑251 (MQ=255)                                                                                                                                                                                      agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:372749/201‑1 (MQ=255)                                                                                                                                                                                      agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:306939/1‑251 (MQ=255)                                                                                                                                                                                      agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                                                >  1:603477/1‑251 (MQ=255)                                                                                                                                                                                       gCTCATACTCTCCACGGTGGAAACAATATTTGCTTCGCAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAgg                                               >  1:32216/1‑251 (MQ=255)                                                                                                                                                                                        cTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                                      >  1:895558/1‑227 (MQ=255)                                                                                                                                                                                          cATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTa                                            >  1:495735/1‑251 (MQ=255)                                                                                                                                                                                             actctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:587004/5‑194 (MQ=255)                                                                                                                                                                                              ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                                        <  1:59247/248‑1 (MQ=255)                                                                                                                                                                                              ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                                        <  1:350774/248‑1 (MQ=255)                                                                                                                                                                                                ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcgc                                                                                           >  1:427095/2‑198 (MQ=255)                                                                                                                                                                                                ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCcggtc                                                      >  1:830797/2‑235 (MQ=255)                                                                                                                                                                                                ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                                      >  1:724952/2‑251 (MQ=255)                                                                                                                                                                                                ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                                      >  1:728930/2‑251 (MQ=255)                                                                                                                                                                                                       gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCGCCATATAAAc                                                                                                  <  1:86324/184‑1 (MQ=255)                                                                                                                                                                                                       gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTgg                                       >  1:816596/1‑243 (MQ=255)                                                                                                                                                                                                       gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATa                               >  1:854013/1‑251 (MQ=255)                                                                                                                                                                                                         ggAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACg                             >  1:92052/1‑251 (MQ=255)                                                                                                                                                                                                           aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc                           <  1:234735/251‑2 (MQ=255)                                                                                                                                                                                                           aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc                           <  1:639592/251‑2 (MQ=255)                                                                                                                                                                                                           aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc                            >  1:42943/1‑250 (MQ=255)                                                                                                                                                                                                           aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc                            <  1:515667/250‑1 (MQ=255)                                                                                                                                                                                                              cAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttg                        >  1:656982/1‑247 (MQ=255)                                                                                                                                                                                                                atatTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttgag                      <  1:358342/251‑7 (MQ=255)                                                                                                                                                                                                                         tCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  <  1:674913/166‑1 (MQ=255)                                                                                                                                                                                                                              agAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCttttggc                                                                                                                                                                                           <  1:679439/71‑4 (MQ=255)                                                                                                                                                                                                                                  gtgaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgaga    >  1:142724/4‑248 (MQ=255)                                                                                                                                                                                                                                    gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttattt                                                                                                              >  1:509456/2‑143 (MQ=255)                                                                                                                                                                                                                                    gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                                                  >  1:760279/2‑155 (MQ=255)                                                                                                                                                                                                                                    gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgagata  >  1:239945/2‑246 (MQ=255)                                                                                                                                                                                                                                    gaTTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGCCTTGAGTTCGTTTTTGTAATgagata  >  1:350373/2‑246 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            GTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAGCTCATTTTTATAATGCGAAA  >  1/1140993‑1141470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑