Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,220	A→G	I140I (ATT→ATC)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,220	0	A	G	100.0%	209.3 / NA	73	I140I (ATT→ATC)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/33);  total (40/33)

CATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAG  >  1/1140974‑1141450                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       cattAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGAttt                                                                                                                                                                                                                                    >  1:176915/1‑251 (MQ=255)  attAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGAttt                                                                                                                                                                                                                                    >  1:619408/1‑250 (MQ=255)      aGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCAc                                                                                                                                                                                                                                 <  1:794316/249‑1 (MQ=255)       gCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCtt                                                                                                                                                                                                                              >  1:18114/1‑251 (MQ=255)       gCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCtt                                                                                                                                                                                                                              >  1:772400/1‑251 (MQ=255)          taggTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGa                                                                                                                                                                                                                            >  1:506760/3‑250 (MQ=255)                    gTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAAttttt                                                                                                                                                                                                                 >  1:645212/1‑248 (MQ=255)                                  gAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaaa                                                                                                                                                                                                   >  1:190311/1‑251 (MQ=255)                                      aaaTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGtt                                                                                                                                                                                               >  1:252915/1‑251 (MQ=255)                                        aTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGt                                                                                                                                                                                             >  1:49409/1‑251 (MQ=255)                                                   cTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAg                                                                                                                                                                                                  <  1:269481/235‑1 (MQ=255)                                                     ttttGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAg                                                                                                                                                                                >  1:880963/1‑251 (MQ=255)                                                               gCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCa                                                                                                                                                                      >  1:827840/1‑251 (MQ=255)                                                                    gCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcg                                                                                                                                                                  >  1:405385/1‑250 (MQ=255)                                                                     cGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcgcg                                                                                                                                                                <  1:746470/251‑1 (MQ=255)                                                                         cAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTa                                                                                                                                                            >  1:671825/1‑251 (MQ=255)                                                                            cTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAATGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATg                                                                                                                                                         >  1:447650/1‑251 (MQ=255)                                                                                         gCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCAc                                                                                                                                             >  1:482867/1‑250 (MQ=255)                                                                                              ttttAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGaaa                                                                                                                                       >  1:805680/1‑251 (MQ=255)                                                                                                    gTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaa                                                                                                                                                                                                    >  1:523565/1‑184 (MQ=255)                                                                                                     tCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAt                                                                                                                                 <  1:58629/250‑1 (MQ=255)                                                                                                                                         cgtttagcgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                            >  1:673838/13‑251 (MQ=255)                                                                                                                                                cgagtttATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAcc                                                                                      <  1:690135/245‑1 (MQ=255)                                                                                                                                                       atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAGATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              <  1:812671/232‑1 (MQ=255)                                                                                                                                                       atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              <  1:299863/232‑1 (MQ=255)                                                                                                                                                       atcaatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTGGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              <  1:426661/232‑1 (MQ=255)                                                                                                                                                         caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                            <  1:850073/234‑1 (MQ=255)                                                                                                                                                         caatagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                            <  1:329674/234‑1 (MQ=255)                                                                                                                                                            tagttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                         <  1:757994/237‑1 (MQ=255)                                                                                                                                                              gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCg                                                                                                                                                       <  1:607727/159‑1 (MQ=255)                                                                                                                                                              gttttcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcg                                                                        >  1:719511/13‑250 (MQ=255)                                                                                                                                                                  tcaatttacTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCAc                                                                                       <  1:96845/223‑1 (MQ=255)                                                                                                                                                                         acTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              >  1:675182/2‑233 (MQ=255)                                                                                                                                                                          cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTc                                                                                                                                                                                      >  1:451876/1‑128 (MQ=255)                                                                                                                                                                          cTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCatttat                                                                                            <  1:211021/218‑1 (MQ=255)                                                                                                                                                                             ttGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCATCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGc                                                        <  1:441807/251‑1 (MQ=255)                                                                                                                                                                              tGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCt                                                       <  1:600379/251‑1 (MQ=255)                                                                                                                                                                                 cgttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgc                                                                         >  1:487590/3‑230 (MQ=255)                                                                                                                                                                                   ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                  <  1:548180/251‑1 (MQ=255)                                                                                                                                                                                   ttACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                  <  1:412725/251‑1 (MQ=255)                                                                                                                                                                                      cTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                         <  1:263973/209‑1 (MQ=255)                                                                                                                                                                                        ttCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCAc                                             <  1:317149/251‑1 (MQ=255)                                                                                                                                                                                                aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACTATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACt                                                                             >  1:357477/1‑211 (MQ=255)                                                                                                                                                                                                aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATat                                                                                  <  1:254252/206‑2 (MQ=255)                                                                                                                                                                                                aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTa                                                                            <  1:34871/212‑1 (MQ=255)                                                                                                                                                                                                aaTCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTccg                                     >  1:632917/1‑250 (MQ=255)                                                                                                                                                                                                     aagaagCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTc                                                                                         <  1:665406/194‑1 (MQ=255)                                                                                                                                                                                                         agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCGCCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                            >  1:325610/1‑251 (MQ=255)                                                                                                                                                                                                         agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              <  1:372749/201‑1 (MQ=255)                                                                                                                                                                                                         agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                            >  1:603477/1‑251 (MQ=255)                                                                                                                                                                                                         agCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAg                            >  1:306939/1‑251 (MQ=255)                                                                                                                                                                                                          gCTCATACTCTCCACGGTGGAAACAATATTTGCTTCGCAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAgg                           >  1:32216/1‑251 (MQ=255)                                                                                                                                                                                                           cTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTAc                                                  >  1:895558/1‑227 (MQ=255)                                                                                                                                                                                                             cATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTa                        >  1:495735/1‑251 (MQ=255)                                                                                                                                                                                                                actctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              >  1:587004/5‑194 (MQ=255)                                                                                                                                                                                                                 ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                    <  1:350774/248‑1 (MQ=255)                                                                                                                                                                                                                 ctctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTg                    <  1:59247/248‑1 (MQ=255)                                                                                                                                                                                                                   ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATgcgc                                                                       >  1:427095/2‑198 (MQ=255)                                                                                                                                                                                                                   ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCcggtc                                  >  1:830797/2‑235 (MQ=255)                                                                                                                                                                                                                   ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                  >  1:728930/2‑251 (MQ=255)                                                                                                                                                                                                                   ctCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGt                  >  1:724952/2‑251 (MQ=255)                                                                                                                                                                                                                          gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCGCCATATAAAc                                                                              <  1:86324/184‑1 (MQ=255)                                                                                                                                                                                                                          gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTgg                   >  1:816596/1‑243 (MQ=255)                                                                                                                                                                                                                          gTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATa           >  1:854013/1‑251 (MQ=255)                                                                                                                                                                                                                            ggAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACg         >  1:92052/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc       <  1:234735/251‑2 (MQ=255)                                                                                                                                                                                                                              aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGcc       <  1:639592/251‑2 (MQ=255)                                                                                                                                                                                                                              aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc        <  1:515667/250‑1 (MQ=255)                                                                                                                                                                                                                              aaaCAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGc        >  1:42943/1‑250 (MQ=255)                                                                                                                                                                                                                                 cAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttg    >  1:656982/1‑247 (MQ=255)                                                                                                                                                                                                                                   atatTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCGGTCTGTGAGGCTAATTGGTCGGCATACGccttgag  <  1:358342/251‑7 (MQ=255)                                                                                                                                                                                                                                            tCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAACCATTTATTTCACCATATAAAc                                                                              <  1:674913/166‑1 (MQ=255)                                                                                                                                                                                                                                                 agAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCttttggc                                                                                                                                                                       <  1:679439/71‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       CATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAATCGATTACCGGGGTAAACTCCGATTTTAAGCCATTTATTTCACCATACAAACTATGCGCTTCACCAATAAAGGCTTGTACATCACGAATTCCTGTCTGTGAGGCTAATTGGTCGGCATACGCTTTCAG  >  1/1140974‑1141450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑