Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,088	T→G	V184V (GTA→GTC)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,088	0	T	G	100.0%	182.9 / NA	64	V184V (GTA→GTC)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/24);  total (40/24)

TCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAT  >  1/1140842‑1141323                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            tctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACAt                                                                                                                                                                                                                                         >  1:231767/1‑251 (MQ=255)    gAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATctt                                                                                                                                                                                                                                      >  1:742822/1‑249 (MQ=255)           cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattt                                                                                                                                                                                                                               >  1:403913/1‑242 (MQ=255)           cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagatt                                                                                                                                                                                                                                <  1:121813/250‑9 (MQ=255)           cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgag                                                                                                                                                                                                                                   >  1:256274/1‑242 (MQ=255)                   gtgaaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatc                                                                                                                                                                                                                        >  1:434598/4‑234 (MQ=255)                    tgaaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTATAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatc                                                                                                                                                                                                                        <  1:181258/247‑17 (MQ=255)                      aaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatccgtt                                                                                                                                                                                                                    >  1:157157/1‑231 (MQ=255)                      aaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatccgtt                                                                                                                                                                                                                    >  1:869553/1‑231 (MQ=255)                                                                      gttGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCaa                                                                                                                                                                    >  1:806231/2‑251 (MQ=255)                                                                      gttGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCaa                                                                                                                                                                    >  1:256227/2‑251 (MQ=255)                                                                         gAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAAc                                                                                                                                                                  >  1:548839/1‑250 (MQ=255)                                                                          aaTATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACg                                                                                                                                                                 <  1:517490/250‑1 (MQ=255)                                                                                   cccTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatc                                                                                                                                                                                                                        <  1:730512/186‑17 (MQ=255)                                                                                   cccTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTaaga                                                                                                                                                        <  1:14967/250‑1 (MQ=255)                                                                                   cccTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTaaga                                                                                                                                                        <  1:364191/250‑1 (MQ=255)                                                                                     cTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTaagaag                                                                                                                                                      >  1:427744/1‑250 (MQ=255)                                                                                            tttaaGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATAc                                                                                                                                               <  1:146889/246‑1 (MQ=255)                                                                                            tttaaGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATAc                                                                                                                                               <  1:322559/246‑1 (MQ=255)                                                                                             ttaaGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACtc                                                                                                                                             <  1:572981/248‑2 (MQ=255)                                                                                                    aCGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCaaa                                                                                                                                                                   >  1:883047/1‑222 (MQ=255)                                                                                                    aCGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACgg                                                                                                                                      <  1:295796/251‑1 (MQ=255)                                                                                                     cGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGt                                                                                                                                     <  1:396846/251‑1 (MQ=255)                                                                                                     cGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGt                                                                                                                                     <  1:549341/251‑1 (MQ=255)                                                                                                     cGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGt                                                                                                                                     <  1:592759/251‑1 (MQ=255)                                                                                                      gTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTg                                                                                                                                    >  1:361700/1‑251 (MQ=255)                                                                                                           gaCGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagat                                                                                                                                                                                                                                 <  1:92983/153‑8 (MQ=255)                                                                                                             cGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACaa                                                                                                                             <  1:95732/251‑1 (MQ=255)                                                                                                             cGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACaa                                                                                                                             <  1:482896/251‑1 (MQ=255)                                                                                                               cTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAata                                                                                                                           >  1:778239/1‑251 (MQ=255)                                                                                                                tAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAatat                                                                                                                          <  1:557417/251‑1 (MQ=255)                                                                                                                   cTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTg                                                                                                                       >  1:879705/1‑251 (MQ=255)                                                                                                                    tGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGc                                                                                                                      <  1:407658/251‑1 (MQ=255)                                                                                                                             tgCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTcacaaga                                                                                                             >  1:68357/1‑248 (MQ=255)                                                                                                                                     cattAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagat                                                                                                                                                                                                                                 >  1:244536/1‑120 (MQ=255)                                                                                                                                     cattAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGt                                                                                                                                     >  1:246456/1‑219 (MQ=255)                                                                                                                                     cattAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGAttt                                                                                                     >  1:176915/1‑251 (MQ=255)                                                                                                                                      attAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGAttt                                                                                                     >  1:619408/1‑250 (MQ=255)                                                                                                                                          aGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCAc                                                                                                  <  1:794316/249‑1 (MQ=255)                                                                                                                                           gCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCtt                                                                                               >  1:18114/1‑251 (MQ=255)                                                                                                                                           gCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCtt                                                                                               >  1:772400/1‑251 (MQ=255)                                                                                                                                              taggTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGa                                                                                             >  1:506760/3‑250 (MQ=255)                                                                                                                                                 gTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTaaga                                                                                                                                                        <  1:214849/188‑1 (MQ=255)                                                                                                                                                        gTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAAttttt                                                                                  >  1:645212/1‑248 (MQ=255)                                                                                                                                                                      gAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaaa                                                                    >  1:190311/1‑251 (MQ=255)                                                                                                                                                                          aaaTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGtt                                                                >  1:252915/1‑251 (MQ=255)                                                                                                                                                                            aTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATAc                                                                                                                                               >  1:614596/1‑170 (MQ=255)                                                                                                                                                                            aTCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGt                                                              >  1:49409/1‑251 (MQ=255)                                                                                                                                                                                       cTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAg                                                                   <  1:269481/235‑1 (MQ=255)                                                                                                                                                                                         ttttGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAg                                                 >  1:880963/1‑251 (MQ=255)                                                                                                                                                                                                 ttGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATAc                                                                                                                                               >  1:4547/1‑149 (MQ=255)                                                                                                                                                                                                 ttGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGt                                                                                                                                     >  1:515217/1‑159 (MQ=255)                                                                                                                                                                                                   gCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCa                                       >  1:827840/1‑251 (MQ=255)                                                                                                                                                                                                        gCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcg                                   >  1:405385/1‑250 (MQ=255)                                                                                                                                                                                                         cGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTcgcg                                 <  1:746470/251‑1 (MQ=255)                                                                                                                                                                                                           gCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTgg                                                                                                                                   >  1:520275/1‑151 (MQ=255)                                                                                                                                                                                                             cAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTa                             >  1:671825/1‑251 (MQ=255)                                                                                                                                                                                                               aCTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTg                                                                                                                       >  1:554918/1‑159 (MQ=255)                                                                                                                                                                                                                cTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAATGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATg                          >  1:447650/1‑251 (MQ=255)                                                                                                                                                                                                                             gCATTTTTTAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCAc              >  1:482867/1‑250 (MQ=255)                                                                                                                                                                                                                                  ttttAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAata                                                                                                                           <  1:721711/136‑1 (MQ=255)                                                                                                                                                                                                                                  ttttAAGTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGaaa        >  1:805680/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCaaaa                                                                     >  1:523565/1‑184 (MQ=255)                                                                                                                                                                                                                                         tCCTGGCTTTCTTTGACATCTTGAGATTTTGTCATCCGTTTAGCGAGTTTATCAATAGTTTTCAATTTACTATTGACGTTACTTTCAAACGAATCTAAGAAGCTCATACTCTCCACGGTGGAAACAATATTTGCTTCACAAGAAGTGATTTCACCTTGACGCAAATTTTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTGGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAt  <  1:58629/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            TCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGATTAGCTAACTTATCAATTGCTTTAAGTTTGCTATTGACATTACTTTCAAACGAATCTAAGAAGCTCATACTTTCCACGGTGGAAACAATATTTGCTTCACAGGAAGTAATTTCACCTTGACGTAAATTCTTACAACGGTCAAAAAGGTTGTATTTTTCAAACAGGCTTTTAGCATTCGCGCTTAATGCGTTTTTCCCACCGGAAACTAAAT  >  1/1140842‑1141323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑