Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,911	A→G	N243N (AAT→AAC)	unknown ←	Minor pilin of type IV secretion complex, VirB5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,911	0	A	G	100.0%	128.1 / NA	46	N243N (AAT→AAC)	unknown	Minor pilin of type IV secretion complex, VirB5
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/21);  total (25/21)

AACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGAT  >  1/1140664‑1141113                                                                                                                                                                                                                                                        |                                                                                                                                                                                                           aaCCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTg                                                                                                                                                                                                         <  1:524314/251‑1 (MQ=255)  aCCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGa                                                                                                                                                                                                        >  1:326000/1‑251 (MQ=255)      aaTTAGTAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAatat                                                                                                                                                                                                    <  1:47796/251‑1 (MQ=255)      aaTTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAatat                                                                                                                                                                                                    <  1:584480/251‑1 (MQ=255)            aaaaaTTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGc                                                                                                                                                                                               <  1:229055/250‑1 (MQ=255)                     cacCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                                                                                                                                                                                       <  1:505105/249‑1 (MQ=255)                     cacCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGtt                                                                                                                                                                                     >  1:512594/1‑251 (MQ=255)                                       gTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACg                                                                                                                                                                    >  1:800828/1‑250 (MQ=255)                                                 tgcttgTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGc                                                                                                                                                                                               >  1:743181/1‑213 (MQ=255)                                                        gCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                                                                                                                                                                                       <  1:737249/214‑1 (MQ=255)                                                                              tggagtttgccattaatggctccgCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTc                                                                                                                            <  1:6414/228‑1 (MQ=255)                                                                                       ccattaatggctccgCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCg                                                                                                                   >  1:717084/15‑251 (MQ=255)                                                                                         attaatggctccgCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTg                                                                                                                 >  1:397596/13‑251 (MQ=255)                                                                                                 ctccgCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAAt                                                                                                         <  1:562322/247‑1 (MQ=255)                                                                                                                                 tttGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGc                                                                         <  1:440719/251‑1 (MQ=255)                                                                                                                                              cGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCg                                                                                                                          <  1:797689/189‑1 (MQ=255)                                                                                                                                              cGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACg                                                            <  1:71402/251‑1 (MQ=255)                                                                                                                                                tATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTc                                                          <  1:513579/251‑1 (MQ=255)                                                                                                                                                        ttGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCAt                                                  <  1:642599/251‑1 (MQ=255)                                                                                                                                                        ttGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCAt                                                  >  1:671280/1‑251 (MQ=255)                                                                                                                                                        ttGTAATACTCTACGCTTTTAACCTCTTCTAAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCAt                                                  >  1:789229/1‑251 (MQ=255)                                                                                                                                                          gTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGt                                                                                                              <  1:887528/189‑1 (MQ=255)                                                                                                                                                          gTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCAttt                                                >  1:794007/1‑251 (MQ=255)                                                                                                                                                               actctacgcTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATg                                                                          >  1:707196/9‑220 (MQ=255)                                                                                                                                                                    acgcTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAat                                                                                                                                                                                                      >  1:862852/4‑91 (MQ=255)                                                                                                                                                                       cTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                                                                                                                                                                                       >  1:664226/1‑103 (MQ=255)                                                                                                                                                                       cTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTgaga                                                                                                                                                                      <  1:36908/120‑1 (MQ=255)                                                                                                                                                                                 cttctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGt                                                                                                                         >  1:810910/1‑155 (MQ=255)                                                                                                                                                                                 cttctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTtg                                                                                     >  1:196661/1‑191 (MQ=255)                                                                                                                                                                                 cttctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTc                                                          <  1:867596/218‑1 (MQ=255)                                                                                                                                                                                 cttctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGctttctttgac                         <  1:751658/251‑4 (MQ=255)                                                                                                                                                                                   tctGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACAt                       >  1:231767/1‑251 (MQ=255)                                                                                                                                                                                      gAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATctt                    >  1:742822/1‑249 (MQ=255)                                                                                                                                                                                             cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                                                                                                                                                                                       >  1:444707/1‑81 (MQ=255)                                                                                                                                                                                             cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCAc                                                             >  1:489380/1‑203 (MQ=255)                                                                                                                                                                                             cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattt             >  1:403913/1‑242 (MQ=255)                                                                                                                                                                                             cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagatt              <  1:121813/250‑9 (MQ=255)                                                                                                                                                                                             cACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgag                 >  1:256274/1‑242 (MQ=255)                                                                                                                                                                                               cTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTc                                        >  1:34677/1‑222 (MQ=255)                                                                                                                                                                                                     gtgaaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatc      >  1:434598/4‑234 (MQ=255)                                                                                                                                                                                                     gtgaaTATAAATAATGCGATAAAAAGTTTTTTCATCCTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCAt                                                  <  1:426833/203‑1 (MQ=255)                                                                                                                                                                                                      tgaaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTATAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatc      <  1:181258/247‑17 (MQ=255)                                                                                                                                                                                                       gaaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCg                                                                                                                                     >  1:531042/2‑121 (MQ=255)                                                                                                                                                                                                        aaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatccgtt  >  1:869553/1‑231 (MQ=255)                                                                                                                                                                                                        aaTATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTGACATcttgagattttgtcatccgtt  >  1:157157/1‑231 (MQ=255)                                                                                                                                                                                                             aaataaTGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTAGGTATTCCGTTTGCCGTGCGTTGAATAAATc                                                                                                    <  1:339691/148‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                           AACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACGCTAACTGTTTTTGTTGCTCATTCATTAAGCGTCGGTATTCCGTTTGCCGTGCGTTGAATAAATCCAACTGGACTTTTTGTGATTGCAATGCGGCCAACTGCACGTCCATTGCATTTTTTAAGTCCTGGCTTTCTTTTACATCGTTTGATTTCGCCATACGAT  >  1/1140664‑1141113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑