Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,760	T→C	E45E (GAA→GAG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,760	0	T	C	98.8%	214.1 / NA	80	E45E (GAA→GAG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (35/44);  minor base A (1/0);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACG  >  1/1140523‑1140951                                                                                                                                                                                                                                              |                                                                                                                                                                                                ttttCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTtct                                                                                                                                                                                    <  1:407200/251‑1 (MQ=255)             gTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTa                                                                                                                                                                        >  1:136938/1‑251 (MQ=255)                   tGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACAttt                                                                                                                                                                  >  1:78536/1‑251 (MQ=255)                               gCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAg                                                                                                                                                      >  1:191853/1‑251 (MQ=255)                                    taatTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGAc                                                                                                                                                            >  1:858491/1‑240 (MQ=255)                                         ttgccgtaatatctggCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTc                                                                                                                                            <  1:593204/236‑1 (MQ=255)                                                          aaaaCTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                                                                                                                           <  1:898320/251‑6 (MQ=255)                                                              ctGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCtttt                                                                                                                       >  1:148409/2‑251 (MQ=255)                                                              ctGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCtttt                                                                                                                       >  1:429156/2‑251 (MQ=255)                                                                  tACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAcc                                                                                                                   <  1:617465/251‑2 (MQ=255)                                                                   aCTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAAt                                                                                                                                    <  1:31945/233‑1 (MQ=255)                                                                   aCTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAc                                                                                                                    >  1:108910/1‑249 (MQ=255)                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                                                                                                                           <  1:685145/241‑6 (MQ=255)                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTc                                                                                                                                <  1:339064/236‑1 (MQ=255)                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCttt                                                                                                                        <  1:514144/244‑1 (MQ=255)                                                                       aTAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  >  1:94588/1‑231 (MQ=255)                                                                        tAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  <  1:564909/230‑1 (MQ=255)                                                                         aGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAcctctt                                                                                                               >  1:616641/1‑248 (MQ=255)                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:755239/251‑1 (MQ=255)                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:510211/251‑1 (MQ=255)                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:163358/251‑1 (MQ=255)                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGt                                                                                                                                              <  1:212568/212‑1 (MQ=255)                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:592640/249‑1 (MQ=255)                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         >  1:522327/1‑249 (MQ=255)                                                                                   aCTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATttttct                                                                                                                                                                         <  1:657488/180‑1 (MQ=255)                                                                                      gaatctagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTaa                                                                                               >  1:468944/9‑251 (MQ=255)                                                                                       aatctagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTaa                                                                                               <  1:829647/243‑1 (MQ=255)                                                                                        atctagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAg                                                                                             <  1:489736/245‑1 (MQ=255)                                                                                        atctagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAg                                                                                             <  1:832829/245‑1 (MQ=255)                                                                                        atctagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAg                                                                                             <  1:470046/245‑1 (MQ=255)                                                                                           tagtAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGAATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGATCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         >  1:91494/4‑236 (MQ=255)                                                                                              tAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAata                                                                                       >  1:26331/1‑251 (MQ=255)                                                                                                    aaTAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  <  1:802634/250‑1 (MQ=255)                                                                                                    aaTAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  <  1:434107/250‑1 (MQ=255)                                                                                                         cGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:132721/222‑1 (MQ=255)                                                                                                                  cGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAcc                                                                                                                   >  1:731728/1‑202 (MQ=255)                                                                                                                       ctctCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGt                                                                                                                                              >  1:272002/1‑171 (MQ=255)                                                                                                                       ctctCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  >  1:768664/1‑183 (MQ=255)                                                                                                                       ctctCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  <  1:721465/183‑1 (MQ=255)                                                                                                                       ctctCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  >  1:169242/1‑183 (MQ=255)                                                                                                                       ctctCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                                                                                                                           >  1:19210/1‑185 (MQ=255)                                                                                                                         ctcAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgct                                                                                                                          <  1:717610/189‑7 (MQ=255)                                                                                                                         ctcAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCa                                                            >  1:288558/1‑251 (MQ=255)                                                                                                                           cAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                                                                                                                           >  1:264438/1‑181 (MQ=255)                                                                                                                                 aTCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:742845/198‑1 (MQ=255)                                                                                                                                   ctcATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  <  1:160224/219‑1 (MQ=255)                                                                                                                                   ctcATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGc                                                                             <  1:225026/224‑1 (MQ=255)                                                                                                                                     cATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGtg                                                                                           >  1:134306/1‑207 (MQ=255)                                                                                                                                     cATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  >  1:328825/1‑217 (MQ=255)                                                                                                                                     cATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  >  1:304672/1‑217 (MQ=255)                                                                                                                                        aaTGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCatat                                             >  1:649048/1‑251 (MQ=255)                                                                                                                                         aTGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCa                                                                                                    <  1:569840/195‑1 (MQ=255)                                                                                                                                         aTGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  >  1:198681/1‑213 (MQ=255)                                                                                                                                         aTGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  >  1:325351/1‑213 (MQ=255)                                                                                                                                         aTGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATAtt                                            >  1:349590/1‑251 (MQ=255)                                                                                                                                           gATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  >  1:796502/1‑163 (MQ=255)                                                                                                                                            aTAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTag                                          <  1:384385/250‑2 (MQ=255)                                                                                                                                              aaCCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  <  1:588218/160‑1 (MQ=255)                                                                                                                                              aaCCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTg                                       <  1:524314/251‑1 (MQ=255)                                                                                                                                               aCCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGa                                      >  1:326000/1‑251 (MQ=255)                                                                                                                                                   aaTTAGTAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAatat                                  <  1:47796/251‑1 (MQ=255)                                                                                                                                                   aaTTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAatat                                  <  1:584480/251‑1 (MQ=255)                                                                                                                                                         aaaaaTTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGc                             <  1:229055/250‑1 (MQ=255)                                                                                                                                                            aaTTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         <  1:826253/171‑1 (MQ=255)                                                                                                                                                                  cacCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                     <  1:505105/249‑1 (MQ=255)                                                                                                                                                                  cacCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGtt                   >  1:512594/1‑251 (MQ=255)                                                                                                                                                                       cGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTc                                                                                                                                            <  1:626447/125‑1 (MQ=255)                                                                                                                                                                          gTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTAt                                                                                                                                                <  1:794157/118‑1 (MQ=255)                                                                                                                                                                            aTTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGtg                                                                                           <  1:371754/169‑2 (MQ=255)                                                                                                                                                                             ttCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc                                                                                                         >  1:647249/1‑154 (MQ=255)                                                                                                                                                                             ttCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATataaat                                                                                  >  1:223406/1‑177 (MQ=255)                                                                                                                                                                             ttCATACGTCGAATACTTGCTTGTGCTTTATTAGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                                                                                                                                  >  1:95643/1‑129 (MQ=255)                                                                                                                                                                                   cGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAcctg                                                                                                                               <  1:712240/126‑4 (MQ=255)                                                                                                                                                                                    gTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTaa                                                                                               >  1:346764/1‑157 (MQ=255)                                                                                                                                                                                    gTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACGTTTAAGCGACGTTGAGACg  >  1:800828/1‑250 (MQ=255)                                                                                                                                                                                       gAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTagtt                                        <  1:324995/209‑4 (MQ=255)                                                                                                                                                                                         aTACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAAt                                                                                                                                    <  1:875213/115‑1 (MQ=255)                                                                                                                                                                                              tgcttgTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGc                             >  1:743181/1‑213 (MQ=255)                                                                                                                                                                                                     gCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAACAGGCACTTAAAGTGAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCATATTAGTTGAATATAACGCCCTTAACg                     <  1:737249/214‑1 (MQ=255)                                                                                                                                                                                                         tATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctac                                                                                                                             <  1:382891/106‑4 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                TTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAACAAGCACTTAAAGTTAATATAAATAATGCGATAAAAAGTTTTTTCATCTTTTACTCCGTATTAATTGAATATAACGCCCTTAACGTTTGAGCGACGTTGAGACG  >  1/1140523‑1140951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑