Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,615	A→G	L15L (TTG→CTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,615	0	A	G	100.0%	221.6 / NA	77	L15L (TTG→CTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/39);  total (38/39)

TTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAAC  >  1/1140379‑1140848                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          tttaagaTCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                                                                                                                                                                             <  1:223851/247‑1 (MQ=255)            caATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                                                                                                                                                                                  <  1:383339/251‑1 (MQ=255)             aaTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                                                                                                                                                                                 <  1:561047/251‑1 (MQ=255)              aTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                                                                                                                                                                                  >  1:775996/1‑249 (MQ=255)                     atAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                                                                                                                                                                         >  1:868717/2‑251 (MQ=255)                          gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAAt                                                                                                                                                                                                      >  1:687334/1‑249 (MQ=255)                            gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAAt                                                                                                                                                                                                                                  >  1:230326/1‑219 (MQ=255)                            gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                                                                                                                                                                                  >  1:546754/1‑251 (MQ=255)                            gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                                                                                                                                                                                  >  1:608627/1‑251 (MQ=255)                             ttATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAt                                                                                                                                                                                                 <  1:724366/251‑1 (MQ=255)                               atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                                                                                                                                                                                 >  1:603979/5‑233 (MQ=255)                               atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                                                                                                                                                                               >  1:142177/5‑251 (MQ=255)                               atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                                                                                                                                                                               >  1:33248/5‑251 (MQ=255)                                tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                                                                                                                                                                             <  1:560229/217‑1 (MQ=255)                                tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAAt                                                                                                                                                                                              >  1:117896/4‑251 (MQ=255)                                   aGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAAt                                                                                                                                                                                                                       >  1:899676/1‑223 (MQ=255)                                        ccccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCt                                                                                                                                                                                      <  1:417582/247‑1 (MQ=255)                                         cccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                                                                                                                                                                         >  1:882512/4‑231 (MQ=255)                                          ccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAAcc                                                                                                                                                                                       >  1:689464/3‑248 (MQ=255)                                            ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAAtt                                                                                                                                                                                  >  1:400285/1‑251 (MQ=255)                                              aTAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAg                                                                                                                                                                                >  1:402433/1‑251 (MQ=255)                                                                  cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa                                                                                                                                                            <  1:712516/251‑1 (MQ=255)                                                                  cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa                                                                                                                                                            >  1:103477/1‑251 (MQ=255)                                                                    ataaTCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTAtt                                                                                                                                                          >  1:894302/4‑251 (MQ=255)                                                                        tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa                                                                                                                                                      <  1:679291/251‑1 (MQ=255)                                                                        tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa                                                                                                                                                      <  1:874965/251‑1 (MQ=255)                                                                                     aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                                                                         <  1:375826/251‑1 (MQ=255)                                                                                     aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                                                                         <  1:78949/251‑1 (MQ=255)                                                                                     aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                                                                         <  1:225970/251‑1 (MQ=255)                                                                                        aTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtgctt                                                                                                                                      >  1:641640/1‑251 (MQ=255)                                                                                        aTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtgc                                                                                                                                        >  1:233181/1‑249 (MQ=255)                                                                                        aTCATAAATCAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAAtt                                                                                                                                                                         <  1:476538/216‑1 (MQ=255)                                                                                         tCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACg                                                                                                                                                                                                                              <  1:572285/162‑1 (MQ=255)                                                                                                ttAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGAt                                                                                                                                                                                           >  1:894348/1‑190 (MQ=255)                                                                                                ttAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTa                                                                                                                              >  1:16334/1‑251 (MQ=255)                                                                                                      cTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGca                                                                                                                        <  1:23569/251‑2 (MQ=255)                                                                                                        gTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGcatt                                                                                                                      >  1:583683/1‑248 (MQ=255)                                                                                                          tGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGt                                                                                                                                                   <  1:632170/220‑1 (MQ=255)                                                                                                             tGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGcattactac                                                                                                                 <  1:660035/251‑9 (MQ=255)                                                                                                                  cTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttg                                                                                                            <  1:112412/251‑2 (MQ=255)                                                                                                                  cTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttg                                                                                                            <  1:175638/251‑2 (MQ=255)                                                                                                                     cAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttggag                                                                                                         <  1:159639/251‑5 (MQ=255)                                                                                                                              cAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAtt                                                                                                >  1:476506/1‑250 (MQ=255)                                                                                                                                gATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAttaa                                                                                              >  1:382966/1‑248 (MQ=255)                                                                                                                                 atatTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGca                                                                                                                        <  1:830391/224‑2 (MQ=255)                                                                                                                                 atatTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAttaat                                                                                             >  1:6411/1‑247 (MQ=255)                                                                                                                                             aTCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATAc                                                                                                                                            >  1:455285/1‑192 (MQ=255)                                                                                                                                                 ttttCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTtct                                                                             <  1:407200/251‑1 (MQ=255)                                                                                                                                                             gTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTa                                                                 >  1:136938/1‑251 (MQ=255)                                                                                                                                                                   tGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACAttt                                                           >  1:78536/1‑251 (MQ=255)                                                                                                                                                                    gTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttggag                                                                                                         >  1:39186/1‑200 (MQ=255)                                                                                                                                                                               gCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAg                                               >  1:191853/1‑251 (MQ=255)                                                                                                                                                                                    taatTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGAc                                                     >  1:858491/1‑240 (MQ=255)                                                                                                                                                                                         ttgccgtaatatctggCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTc                                     <  1:593204/236‑1 (MQ=255)                                                                                                                                                                                             aaaGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGAt                                                                                                                                                                                           >  1:286516/1‑97 (MQ=255)                                                                                                                                                                                                         cAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAgcac                                                                                                                                                                    <  1:848996/108‑1 (MQ=255)                                                                                                                                                                                                          aaaaCTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                    <  1:898320/251‑6 (MQ=255)                                                                                                                                                                                                              ctGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCtttt                >  1:429156/2‑251 (MQ=255)                                                                                                                                                                                                              ctGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCtttt                >  1:148409/2‑251 (MQ=255)                                                                                                                                                                                                                  tACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAcc            <  1:617465/251‑2 (MQ=255)                                                                                                                                                                                                                   aCTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAAt                             <  1:31945/233‑1 (MQ=255)                                                                                                                                                                                                                   aCTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAc             >  1:108910/1‑249 (MQ=255)                                                                                                                                                                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTctacgc                    <  1:685145/241‑6 (MQ=255)                                                                                                                                                                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTc                         <  1:339064/236‑1 (MQ=255)                                                                                                                                                                                                                    cTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCttt                 <  1:514144/244‑1 (MQ=255)                                                                                                                                                                                                                       aTAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                           >  1:94588/1‑231 (MQ=255)                                                                                                                                                                                                                        tAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATAc                           <  1:564909/230‑1 (MQ=255)                                                                                                                                                                                                                         aGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAAcctctt        >  1:616641/1‑248 (MQ=255)                                                                                                                                                                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActt                                                                                                                                          <  1:754243/115‑1 (MQ=255)                                                                                                                                                                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc  <  1:755239/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc  <  1:510211/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc  <  1:163358/251‑1 (MQ=255)                                                                                                                                                                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGt                                       <  1:212568/212‑1 (MQ=255)                                                                                                                                                                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc  <  1:592640/249‑1 (MQ=255)                                                                                                                                                                                                                              gACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCTACGCTTTTAACCTCTTCTGAAc  >  1:522327/1‑249 (MQ=255)                                                                                                                                                                                                                                  aaCTGAATCTAGTAACTGGATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttggag                                                                                                         <  1:75403/142‑5 (MQ=255)                                                                                                                                                                                                                                   aCTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATttttct                                                                  <  1:657488/180‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          TTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAGTCGTATGTTCTTTGTAATACTCCACACTTTTAACTTCTTCTGAAC  >  1/1140379‑1140848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑