Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,577	A→G	A27A (GCT→GCC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,577	0	A	G	100.0%	210.7 / NA	73	A27A (GCT→GCC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/30);  total (43/30)

TCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAG  >  1/1140332‑1140803                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   catttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaa                                                                                                                                                                                                                               >  1:710751‑M2/43‑251 (MQ=255)                ttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGa                                                                                                                                                                                                                <  1:291681‑M2/224‑1 (MQ=255)                 tgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               <  1:649167‑M2/225‑1 (MQ=255)                     ttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                                                                                                                           <  1:218607‑M2/229‑1 (MQ=255)                           gaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTaa                                                                                                                                                                                                        >  1:26449‑M2/17‑248 (MQ=255)                                              gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               >  1:605751/1‑222 (MQ=255)                                              gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                                                                                                                           >  1:486337/1‑226 (MQ=255)                                                tttaagaTCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                                                                                                                                <  1:223851/247‑1 (MQ=255)                                                        catcaATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagt                                                                                                                                                                                             <  1:484124/230‑5 (MQ=255)                                                           caATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                                                                                                                                     <  1:383339/251‑1 (MQ=255)                                                            aaTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                                                                                                                                    <  1:561047/251‑1 (MQ=255)                                                             aTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                                                                                                                                     >  1:775996/1‑249 (MQ=255)                                                                    atAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                                                                                                                            >  1:868717/2‑251 (MQ=255)                                                                         gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaaac                                                                                                                                                                                                                             <  1:144544/181‑2 (MQ=255)                                                                         gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagtaac                                                                                                                                                                                          >  1:213634/1‑209 (MQ=255)                                                                         gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAAt                                                                                                                                                         >  1:687334/1‑249 (MQ=255)                                                                         gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAAACCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCAt                                                                                                                                                                                                                   <  1:669837/191‑1 (MQ=255)                                                                           gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAAt                                                                                                                                                                                     >  1:230326/1‑219 (MQ=255)                                                                           gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                                                                                                                                     >  1:608627/1‑251 (MQ=255)                                                                           gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                                                                                                                                     >  1:546754/1‑251 (MQ=255)                                                                            ttATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAt                                                                                                                                                    <  1:724366/251‑1 (MQ=255)                                                                             tATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               >  1:533840/1‑191 (MQ=255)                                                                              atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCAt                                                                                                                                                                                                                   >  1:75221/5‑186 (MQ=255)                                                                              atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                                                                                                                                    >  1:603979/5‑233 (MQ=255)                                                                              atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                                                                                                                                  >  1:33248/5‑251 (MQ=255)                                                                              atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                                                                                                                                  >  1:142177/5‑251 (MQ=255)                                                                               tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                                                                                                                                <  1:560229/217‑1 (MQ=255)                                                                               tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAAt                                                                                                                                                 >  1:117896/4‑251 (MQ=255)                                                                                  aGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAAt                                                                                                                                                                          >  1:899676/1‑223 (MQ=255)                                                                                       ccccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCt                                                                                                                                         <  1:417582/247‑1 (MQ=255)                                                                                        cccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                                                                                                                            >  1:882512/4‑231 (MQ=255)                                                                                         ccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAAcc                                                                                                                                          >  1:689464/3‑248 (MQ=255)                                                                                           ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGa                                                                                                                                                                                                                <  1:135131/176‑1 (MQ=255)                                                                                           ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAAtt                                                                                                                                     >  1:400285/1‑251 (MQ=255)                                                                                             aTAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAg                                                                                                                                   >  1:402433/1‑251 (MQ=255)                                                                                                aTTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                                                                                                                           <  1:315764/176‑1 (MQ=255)                                                                                                   catcatAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagtaac                                                                                                                                                                                          >  1:797260/1‑183 (MQ=255)                                                                                                                 cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa                                                                                                               >  1:103477/1‑251 (MQ=255)                                                                                                                 cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa                                                                                                               <  1:712516/251‑1 (MQ=255)                                                                                                                   ataaTCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTAtt                                                                                                             >  1:894302/4‑251 (MQ=255)                                                                                                                       tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa                                                                                                         <  1:874965/251‑1 (MQ=255)                                                                                                                       tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa                                                                                                         <  1:679291/251‑1 (MQ=255)                                                                                                                                    aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                            <  1:375826/251‑1 (MQ=255)                                                                                                                                    aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                            <  1:78949/251‑1 (MQ=255)                                                                                                                                    aaaaTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtg                                                                                            <  1:225970/251‑1 (MQ=255)                                                                                                                                       aTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtgctt                                                                                         >  1:641640/1‑251 (MQ=255)                                                                                                                                       aTCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATActtgc                                                                                           >  1:233181/1‑249 (MQ=255)                                                                                                                                       aTCATAAATCAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAAtt                                                                                                                            <  1:476538/216‑1 (MQ=255)                                                                                                                                        tCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACg                                                                                                                                                                                 <  1:572285/162‑1 (MQ=255)                                                                                                                                             aaTTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               >  1:306409/1‑127 (MQ=255)                                                                                                                                             aaTTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               >  1:746554/1‑127 (MQ=255)                                                                                                                                               ttAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGAt                                                                                                                                              >  1:894348/1‑190 (MQ=255)                                                                                                                                               ttAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTa                                                                                 >  1:16334/1‑251 (MQ=255)                                                                                                                                                     cTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGca                                                                           <  1:23569/251‑2 (MQ=255)                                                                                                                                                       gTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGcatt                                                                         >  1:583683/1‑248 (MQ=255)                                                                                                                                                         tGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGt                                                                                                      <  1:632170/220‑1 (MQ=255)                                                                                                                                                            tGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGcattactac                                                                    <  1:660035/251‑9 (MQ=255)                                                                                                                                                                 cTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttg                                                               <  1:175638/251‑2 (MQ=255)                                                                                                                                                                 cTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttg                                                               <  1:112412/251‑2 (MQ=255)                                                                                                                                                                    cAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttggag                                                            <  1:159639/251‑5 (MQ=255)                                                                                                                                                                           gCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                                                                                                                               >  1:366225/1‑97 (MQ=255)                                                                                                                                                                             cAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAtt                                                   >  1:476506/1‑250 (MQ=255)                                                                                                                                                                               gATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAttaa                                                 >  1:382966/1‑248 (MQ=255)                                                                                                                                                                                atatTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGca                                                                           <  1:830391/224‑2 (MQ=255)                                                                                                                                                                                atatTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCAttaat                                                >  1:6411/1‑247 (MQ=255)                                                                                                                                                                                            aTCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATAc                                                                                               >  1:455285/1‑192 (MQ=255)                                                                                                                                                                                                ttttCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTtct                                <  1:407200/251‑1 (MQ=255)                                                                                                                                                                                                            gTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTa                    >  1:136938/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACAttt              >  1:78536/1‑251 (MQ=255)                                                                                                                                                                                                                   gTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAAttggag                                                            >  1:39186/1‑200 (MQ=255)                                                                                                                                                                                                                              gCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGACGTTCAg  >  1:191853/1‑251 (MQ=255)                                                                                                                                                                                                                                   taatTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCATTACTACAATTGGAGTTTGCCATTAATGGCTCCGCTTTTTTCTCACATTTTTCTAACATTTTTTGAc        >  1:858491/1‑240 (MQ=255)                                                                                                                                                                                                                                            aaaGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGAt                                                                                                                                              >  1:286516/1‑97 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   TCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATACGTCGAATACTTGCTTGTGCTTTATTTGCGTTTCGACAATTAGAATTTGCCATCAGCGGTTCGGCTTTTTTCTCACATTCTTCTAACATTTTTTGACGTTCAG  >  1/1140332‑1140803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑