Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,461	G→A	A66V (GCC→GTC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,461	0	G	A	100.0%	173.5 / NA	65	A66V (GCC→GTC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

GACATATTTAGATTTATCTAGATCAAAGAGTTGACCAGCAATATCCGATCCTAATTCCAATTGGTCATCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATA  >  1/1140265‑1140700                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                                                           <  1:277751‑M2/142‑1 (MQ=255)                                 gttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATGTTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgt                                                                                                                                                           >  1:90798‑M2/78‑251 (MQ=255)                                           gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCCATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTgg                                                                                                                                                                                                                     >  1:148022‑M2/68‑183 (MQ=255)                                           gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                                                           >  1:149953‑M2/68‑209 (MQ=255)                                           gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaa                                                                                                                                                 >  1:37942‑M2/68‑251 (MQ=255)                                            cctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaat                                                                                                                                                <  1:138048‑M2/185‑1 (MQ=255)                                               cctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaataat                                                                                                                                             <  1:54768‑M2/188‑1 (MQ=255)                                               cctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaataat                                                                                                                                             >  1:224522‑M2/64‑251 (MQ=255)                                                                    catttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaa                                                                                                                        >  1:710751‑M2/43‑251 (MQ=255)                                                                                   ttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGa                                                                                                         <  1:291681‑M2/224‑1 (MQ=255)                                                                                    tgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                        <  1:649167‑M2/225‑1 (MQ=255)                                                                                        ttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                    <  1:218607‑M2/229‑1 (MQ=255)                                                                                              gaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTaa                                                                                                 >  1:26449‑M2/17‑248 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTc                                                                                                                                                              >  1:450193/1‑168 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgtcatgc                                                                                                                                                    <  1:836005/178‑6 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTaa                                                                                                                                        <  1:871753/190‑1 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAg                                                                                                                                    <  1:151066/194‑1 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                        >  1:605751/1‑222 (MQ=255)                                                                                                                 gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                    >  1:486337/1‑226 (MQ=255)                                                                                                                   tttaagaTCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                         <  1:223851/247‑1 (MQ=255)                                                                                                                           catcaATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagt                                                                                      <  1:484124/230‑5 (MQ=255)                                                                                                                            atcaATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTg                                                                                                                                           <  1:236837/176‑1 (MQ=255)                                                                                                                              caATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                              <  1:383339/251‑1 (MQ=255)                                                                                                                               aaTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                             <  1:561047/251‑1 (MQ=255)                                                                                                                                aTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                                                              >  1:775996/1‑249 (MQ=255)                                                                                                                                    tcgatAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgtc                                                                                                                                                        <  1:405555/151‑2 (MQ=255)                                                                                                                                      gatAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                                                           <  1:569432/116‑1 (MQ=255)                                                                                                                                       atAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                     >  1:868717/2‑251 (MQ=255)                                                                                                                                            gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgt                                                                                                                                                         <  1:75380/146‑1 (MQ=255)                                                                                                                                            gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaaac                                                                                                                      <  1:144544/181‑2 (MQ=255)                                                                                                                                            gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagtaac                                                                                   >  1:213634/1‑209 (MQ=255)                                                                                                                                            gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAAt                                                  >  1:687334/1‑249 (MQ=255)                                                                                                                                            gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAAACCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCAt                                                                                                            <  1:669837/191‑1 (MQ=255)                                                                                                                                              gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAAt                                                                              >  1:230326/1‑219 (MQ=255)                                                                                                                                              gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                              >  1:608627/1‑251 (MQ=255)                                                                                                                                              gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa                                              >  1:546754/1‑251 (MQ=255)                                                                                                                                               ttATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAt                                             <  1:724366/251‑1 (MQ=255)                                                                                                                                                tATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                                                                        >  1:533840/1‑191 (MQ=255)                                                                                                                                                 atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                                                           <  1:150265/103‑1 (MQ=255)                                                                                                                                                 atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCAt                                                                                                            >  1:75221/5‑186 (MQ=255)                                                                                                                                                 atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                                                             >  1:603979/5‑233 (MQ=255)                                                                                                                                                 atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                           >  1:142177/5‑251 (MQ=255)                                                                                                                                                 atctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATaa                                           >  1:33248/5‑251 (MQ=255)                                                                                                                                                  tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                                                                         <  1:560229/217‑1 (MQ=255)                                                                                                                                                  tctaGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAAt                                          >  1:117896/4‑251 (MQ=255)                                                                                                                                                     aGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAAt                                                                   >  1:899676/1‑223 (MQ=255)                                                                                                                                                       aTACCCCCCATAATTCATCATAAACGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTg                                                                                                                                  <  1:686537/158‑1 (MQ=255)                                                                                                                                                          ccccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTg                                                                                                                                           >  1:891894/5‑146 (MQ=255)                                                                                                                                                          ccccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCt                                  <  1:417582/247‑1 (MQ=255)                                                                                                                                                           cccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg                                                     >  1:882512/4‑231 (MQ=255)                                                                                                                                                            ccccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAAcc                                   >  1:689464/3‑248 (MQ=255)                                                                                                                                                              ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAgc                                                                                                                               >  1:432333/1‑153 (MQ=255)                                                                                                                                                              ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGa                                                                                                         <  1:135131/176‑1 (MQ=255)                                                                                                                                                              ccATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAAtt                              >  1:400285/1‑251 (MQ=255)                                                                                                                                                                aTAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAg                            >  1:402433/1‑251 (MQ=255)                                                                                                                                                                   aTTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAgc                                                                                                                               <  1:10097/149‑2 (MQ=255)                                                                                                                                                                   aTTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                                                                    <  1:315764/176‑1 (MQ=255)                                                                                                                                                                      catcatAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagtaac                                                                                   >  1:797260/1‑183 (MQ=255)                                                                                                                                                                                    cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                                                           >  1:653225/1‑72 (MQ=255)                                                                                                                                                                                    cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa        >  1:103477/1‑251 (MQ=255)                                                                                                                                                                                    cAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTa        <  1:712516/251‑1 (MQ=255)                                                                                                                                                                                      ataaTCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTAtt      >  1:894302/4‑251 (MQ=255)                                                                                                                                                                                        aaTCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTcc                                                                                                                                                                              >  1:683966/2‑81 (MQ=255)                                                                                                                                                                                          tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa  <  1:874965/251‑1 (MQ=255)                                                                                                                                                                                          tCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAGCACCTCGAGTATTCATa  <  1:679291/251‑1 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GACATATTTAGATTTATCTAGATCAAAGAGTTGACCAGCAATATCCGATCCTAATTCCAATTGGTCATCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCATAATGATAACCTAATTAGAAAAATTAACACCTCGAGTATTCATA  >  1/1140265‑1140700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑