Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,140,412	C→T	L82L (CTG→CTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,140,412	0	C	T	100.0%	161.7 / NA	59	L82L (CTG→CTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/32);  total (27/32)

ATGTGATAGTTGTAGTAATAAAAATAATAAATGGCACAAATAAAGATACTGCTACACCAATCCAGGCAAGAAATGAAGCAATTCCACCTTCTAAGGGGACATATTTAGATTTATCTAGATCAAAGAGTTGACCAGCAATATCCGATCCTAATTCCAATTGGTCATCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAT  >  1/1140168‑1140657                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cacggcaagcagtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATAc                                                                                                                                                                                                                                                 <  1:100361‑M2/45‑1 (MQ=255) cacggcaagcagtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATAc                                                                                                                                                                                                                                                 <  1:238080‑M2/45‑1 (MQ=255)   cggcaagcagtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATAc                                                                                                                                                                                                                                                 >  1:142546‑M2/205‑249 (MQ=255)    ggcaagcagtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATAccc                                                                                                                                                                                                                                               >  1:848174‑M2/204‑250 (MQ=255)         gcagtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATacccc                                                                                                                                                                                                                                              >  1:885011‑M2/199‑245 (MQ=255)            gtgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAAt                                                                                                                                                                                                                                       >  1:4241‑M2/196‑250 (MQ=255)             tgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAAt                                                                                                                                                                                                                                       <  1:515339‑M2/55‑1 (MQ=255)             tgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAAt                                                                                                                                                                                                                                       <  1:228092‑M2/55‑1 (MQ=255)             tgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATtc                                                                                                                                                                                                                                     <  1:587990‑M2/57‑1 (MQ=255)             tgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATtc                                                                                                                                                                                                                                     <  1:609080‑M2/57‑1 (MQ=255)             tgcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtgtccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATaa                                                                                                                                                                                                                                        <  1:438952‑M2/54‑1 (MQ=255)              gcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATa                                                                                                                                                                                                                                                  <  1:594226‑M2/44‑1 (MQ=255)              gcaataatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATtca                                                                                                                                                                                                                                    >  1:386138‑M2/194‑251 (MQ=255)                   aatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATa                                                                                                                                                                                                                               >  1:476571‑M2/189‑251 (MQ=255)                     aatcagaaaaataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttaagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATaaa                                                                                                                                                                                                                             >  1:241258‑M2/187‑251 (MQ=255)                            aataaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATAc                                                                                                                                                                                                                                                 <  1:600198‑M2/45‑1 (MQ=255)                              taaatagattttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAAt                                                                                                                                                                                                                    <  1:826185‑M2/74‑1 (MQ=255)                                     attttggtttttaccgcgtttggcaattttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCAtt                                                                                                                                                                                                             <  1:523010‑M2/81‑1 (MQ=255)                                                     cgtttggcaattttgctgacttccggttgataatcggtttccgttttatcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCAtttt                                                                                                                                                                                                           <  1:351355‑M2/83‑1 (MQ=255)                                                              attttgctgacttccggttgataatcggtttccgttttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATtc                                                                                                                                                                                                                                     >  1:91944‑M2/146‑202 (MQ=255)                                                                                                  ttgtcatccgtcggctgaatggttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                <  1:277751‑M2/142‑1 (MQ=255)                                                                                                                       gttttgttatcgttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAAt                                                                                                                                                                                                                                       >  1:879531‑M2/89‑143 (MQ=255)                                                                                                                                  gttcatctctgcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATGTTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgt                                                                                                                >  1:90798‑M2/78‑251 (MQ=255)                                                                                                                                            gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCCATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTgg                                                                                                                                                                          >  1:148022‑M2/68‑183 (MQ=255)                                                                                                                                            gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                >  1:149953‑M2/68‑209 (MQ=255)                                                                                                                                            gcctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaa                                                                                                      >  1:37942‑M2/68‑251 (MQ=255)                                                                                                                                             cctcctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaat                                                                                                     <  1:138048‑M2/185‑1 (MQ=255)                                                                                                                                                cctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaataat                                                                                                  <  1:54768‑M2/188‑1 (MQ=255)                                                                                                                                                cctatcgttcgtctcttgccacatttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCaataat                                                                                                  >  1:224522‑M2/64‑251 (MQ=255)                                                                                                                                                                     catttggcgaaatggttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaa                                                                             >  1:710751‑M2/43‑251 (MQ=255)                                                                                                                                                                                    ttgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGa                                                              <  1:291681‑M2/224‑1 (MQ=255)                                                                                                                                                                                     tgtgttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                             <  1:649167‑M2/225‑1 (MQ=255)                                                                                                                                                                                         ttataagaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                         <  1:218607‑M2/229‑1 (MQ=255)                                                                                                                                                                                               gaaaccggcgttttaaCTAGTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTaa                                                      >  1:26449‑M2/17‑248 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTc                                                                                                                   >  1:450193/1‑168 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgtcatgc                                                                                                         <  1:836005/178‑6 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTaa                                                                                             <  1:871753/190‑1 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAg                                                                                         <  1:151066/194‑1 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                             >  1:605751/1‑222 (MQ=255)                                                                                                                                                                                                                  gTTTTAAGATCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGAc                                                         >  1:486337/1‑226 (MQ=255)                                                                                                                                                                                                                    tttaagaTCATCAATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGa                              <  1:223851/247‑1 (MQ=255)                                                                                                                                                                                                                            catcaATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagt                                           <  1:484124/230‑5 (MQ=255)                                                                                                                                                                                                                             atcaATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTg                                                                                                <  1:236837/176‑1 (MQ=255)                                                                                                                                                                                                                               caATTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                   <  1:383339/251‑1 (MQ=255)                                                                                                                                                                                                                                aaTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATAc                  <  1:561047/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTTATCGATAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATa                   >  1:775996/1‑249 (MQ=255)                                                                                                                                                                                                                                     tcgatAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgtc                                                                                                             <  1:405555/151‑2 (MQ=255)                                                                                                                                                                                                                                       gatAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACgg                                                                                                                                                <  1:569432/116‑1 (MQ=255)                                                                                                                                                                                                                                        atAAAGAGTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAg          >  1:868717/2‑251 (MQ=255)                                                                                                                                                                                                                                             gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAgtgt                                                                                                              <  1:75380/146‑1 (MQ=255)                                                                                                                                                                                                                                             gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCaaaac                                                                           <  1:144544/181‑2 (MQ=255)                                                                                                                                                                                                                                             gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATctagtaac                                        >  1:213634/1‑209 (MQ=255)                                                                                                                                                                                                                                             gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAAt       >  1:687334/1‑249 (MQ=255)                                                                                                                                                                                                                                             gagTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAAACCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCAt                                                                 <  1:669837/191‑1 (MQ=255)                                                                                                                                                                                                                                               gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAAt                                   >  1:230326/1‑219 (MQ=255)                                                                                                                                                                                                                                               gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa   >  1:546754/1‑251 (MQ=255)                                                                                                                                                                                                                                               gTTATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCa   >  1:608627/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGATTGACTAACTGAATCTAGTAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAt  <  1:724366/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tATCTAGATACCCCCCATAATTCATCATAAATGCCACAATAATCATTTTTGTGACAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATTTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTCATGCCAATAATTGTAAAAAGTGAGCTGGCAAAACTGCTACTCATAGAt                                                             >  1:533840/1‑191 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATGTGATAGTTGTAGTAATAAAAATAATAAATGGCACAAATAAAGATACTGCTACACCAATCCAGGCAAGAAATGAAGCAATTCCACCTTCTAAGGGGACATATTTAGATTTATCTAGATCAAAGAGTTGACCAGCAATATCCGATCCTAATTCCAATTGGTCATCCATTAAACCGGCTATACCGTTTCCGGTAAAGCCGGTTAAGCTAGTTTTGAGATCATCAATTATCGCTAAAGAGTTATCCAGATACCCTCCATAATTCATCATAAATGCTACAATGATCATTTTTGTGGCAAAATCATAAATTAATGCTGTTGCTGGAGCTTCAATCTTGCCAGATATTACGGCAAATCCTTTTCCTAAAAAGTAAATTGTCAGTGTTACGCCAATAATTGTAAAAAGTGAGTTAGCAAAATTGCTACTCATAGATTGACTAACTGAATCCAATAACTGAATAACGAAAGAATCGATACTCTCAAAGAATCTCAT  >  1/1140168‑1140657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑