Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,137,913	T→C	T268T (ACA→ACG)	unknown ←	Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,137,913	0	T	C	100.0%	156.2 / NA	58	T268T (ACA→ACG)	unknown	Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

CCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGTGAAATGGTCGTGTTATAAGTTACCGGTGTTTTTCCGTAACTTTGATTGTAAATGCCGACCACTTTTTCACCGCTACGTAATACCAATTTTTCTGCTGTTTTTTGGATGACTAATACCTGATAATTACCGTCTTGTTTAACATTAGTATTTAACAGACTTTCCGTGGAGCCTTCTAACAAGAAGGCTGCAGGAAAGGTTTTATTTGGGGCAAATCCAAGATAAGTAAAAATACCGTCATCATAAGCATAGTTTGGTGTGATGGTTTC  >  1/1137669‑1138152                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ccGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGtttt                                                                                                                                                                                                                                            <  1:867499/250‑1 (MQ=255)      tGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactag                                                                                                                                                                                                                                      >  1:535426‑M1/1‑245 (MQ=255)       gCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaact                                                                                                                                                                                                                                        >  1:581924‑M1/1‑244 (MQ=255)         gTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGttt                                                                                                                                                                                                                                             >  1:624850/1‑241 (MQ=255)           aTCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaact                                                                                                                                                                                                                                        >  1:313499‑M1/1‑240 (MQ=255)              ccttttatTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                                              <  1:727216‑M1/249‑15 (MQ=255)              cTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                                              <  1:722471‑M1/251‑15 (MQ=255)              cTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                                              <  1:498708‑M1/251‑15 (MQ=255)                     ttCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaa                                                                                                                                                                                                                       <  1:300441‑M1/251‑22 (MQ=255)                              gAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTATTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTatctagttttaagatcatcaattatcgat                                                                                                                                                                                                               <  1:376033‑M1/250‑30 (MQ=255)                                    cccAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagt                                                                                                                                                                                                        >  1:281989‑M1/1‑215 (MQ=255)                                         aaaCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatct                                                                                                                                                                                                   <  1:136156‑M1/251‑42 (MQ=255)                                           aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                                                 <  1:204509‑M1/251‑44 (MQ=255)                                           aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                                                 <  1:215261‑M1/251‑44 (MQ=255)                                           aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                                                 <  1:549450‑M1/251‑44 (MQ=255)                                               cACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                                             <  1:238080‑M1/251‑48 (MQ=255)                                               cACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                                             <  1:100361‑M1/251‑48 (MQ=255)                                                 cGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                                             >  1:142546‑M1/1‑202 (MQ=255)                                                  ggCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccc                                                                                                                                                                                           >  1:848174‑M1/1‑201 (MQ=255)                                                  ggCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTACCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatct                                                                                                                                                                                                   >  1:98597‑M1/1‑201 (MQ=255)                                                       gCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatacccc                                                                                                                                                                                          >  1:885011‑M1/1‑196 (MQ=255)                                                        cAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagag                                                                                                                                                                                                         <  1:477613‑M1/230‑36 (MQ=255)                                                          gTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                                                   >  1:4241‑M1/1‑193 (MQ=255)                                                          gTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaatt                                                                                                                                                                                                                     >  1:766993‑M1/1‑193 (MQ=255)                                                           tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                                                 <  1:609080‑M1/251‑60 (MQ=255)                                                           tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                                                 <  1:587990‑M1/251‑60 (MQ=255)                                                           tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                                                   <  1:515339‑M1/249‑58 (MQ=255)                                                           tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                                                   <  1:228092‑M1/249‑58 (MQ=255)                                                           tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTGTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataa                                                                                                                                                                                    <  1:438952‑M1/248‑57 (MQ=255)                                                            gCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattca                                                                                                                                                                                >  1:386138‑M1/1‑191 (MQ=255)                                                            gCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagata                                                                                                                                                                                              <  1:594226‑M1/237‑47 (MQ=255)                                                                 aatCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcata                                                                                                                                                                           >  1:476571‑M1/1‑186 (MQ=255)                                                                 aatCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGT‑‑TAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaa                                                                                                                                                                         >  1:241258‑M1/1‑184 (MQ=255)                                                                        aaaaTAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaatta                                                                                                                                                                                                                    <  1:798024‑M1/203‑25 (MQ=255)                                                                          aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                                             <  1:600198‑M1/224‑48 (MQ=255)                                                                          aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                                                 >  1:803188‑M1/1‑177 (MQ=255)                                                                          aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                                              >  1:78263‑M1/1‑177 (MQ=255)                                                                            taaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaat                                                                                                                                                                <  1:826185‑M1/251‑77 (MQ=255)                                                                                   aTTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatt                                                                                                                                                         <  1:523010‑M1/251‑84 (MQ=255)                                                                                                   cgTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTATCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttt                                                                                                                                                       <  1:351355‑M1/237‑86 (MQ=255)                                                                                                            aTTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                                                 >  1:91944‑M1/1‑143 (MQ=255)                                                                                                                                aTAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagat                                                                                                                                                                                                                             <  1:564690‑M1/138‑16 (MQ=255)                                                                                                                                                ttGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacgg                                                                                            <  1:277751‑M1/251‑145 (MQ=255)                                                                                                                                                  gTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                                              >  1:279010‑M1/1‑105 (MQ=255)                                                                                                                                                                     gTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                                                   >  1:879531‑M1/1‑86 (MQ=255)                                                                                                                                                                          gTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                                                 <  1:892916‑M1/124‑44 (MQ=255)                                                                                                                                                                                gTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatgttacggcaaatccttttcctaaaaagtaaattgtcagt                                                            >  1:90798‑M1/1‑75 (MQ=255)                                                                                                                                                                                          gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatccattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctgg                                                                                                                      >  1:148022‑M1/1‑65 (MQ=255)                                                                                                                                                                                          gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaa                                                  >  1:37942‑M1/1‑65 (MQ=255)                                                                                                                                                                                          gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacgg                                                                                            >  1:149953‑M1/1‑65 (MQ=255)                                                                                                                                                                                           cctcctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaat                                                 <  1:138048‑M1/251‑188 (MQ=255)                                                                                                                                                                                              cctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataat                                              >  1:224522‑M1/1‑61 (MQ=255)                                                                                                                                                                                              cctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataat                                              <  1:54768‑M1/251‑191 (MQ=255)                                                                                                                                                                                                                   caTTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaa                         >  1:710751‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                                  ttgtgttaTAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaaactgctactcataga          <  1:291681‑M1/249‑227 (MQ=255)                                                                                                                                                                                                                                   tgtgttATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaaactgctactcatagat         <  1:649167‑M1/250‑228 (MQ=255)                                                                                                                                                                                                                                       ttATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaaactgctactcatagattgac     <  1:218607‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                                             gaaaccgGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaaactgctactcatagattgactaa  >  1:26449‑M1/4‑14 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGTGAAATGGTCGTGTTATAAGTTACCGGTGTTTTTCCGTAACTTTGATTGTAAATGCCGACCACTTTTTCACCGCTACGTAATACCAATTTTTCTGCTGTTTTTTGGATGACTAATACCTGATAATTACCGTCTTGTTTAACATTAGTATTTAACAGACTTTCCGTGGAGCCTTCTAACAAGAAGGCTGCAGGAAAGGTTTTATTTGGGGCAAATCCAAGATAAGTAAAAATACCGTCATCATAAGCATAGTTTGGTGTGATGGTTTC  >  1/1137669‑1138152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑