Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,137,895	C→T	T274T (ACG→ACA)	unknown ←	Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,137,895	0	C	T	100.0%	160.6 / NA	60	T274T (ACG→ACA)	unknown	Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/32);  total (28/32)

TTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGTGAAATGGTCGTGTTATAAGTTACCGGTGTTTTTCCGTAACTTTGATTGTAAATGCCGACCACTTTTTCACCGCTACGTAATACCAATTTTTCTGCTGTTTTTTGGATGACTAATACCTGATAATTACCGTCTTGTTTAACATTAGTATTTAACAGACTTTCCGTGGAGCCTTCTAACAAGAAGGCTGCAGGAAAGGTTTTATTTGGGGCAAATCCAAGATAAGTAAAAATACCGTCATCATAA  >  1/1137654‑1138129                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           tttCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATaa                                                                                                                                                                                                                                   <  1:460001/251‑1 (MQ=255)      ttAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAgaaac                                                                                                                                                                                                                              <  1:119866/251‑5 (MQ=255)          ccTGTGCCGGTTGCGTATCCTTTTTATTCAATAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAgaaaccgg                                                                                                                                                                                                                           >  1:73374/1‑243 (MQ=255)             gtgCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAgaaaccggcgtt                                                                                                                                                                                                                       >  1:240279/1‑240 (MQ=255)                ccGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGtttt                                                                                                                                                                                                                     <  1:867499/250‑1 (MQ=255)                     tGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAga                                                                                                                                                                                                                                 >  1:629910/1‑232 (MQ=255)                     tGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactag                                                                                                                                                                                                               >  1:535426‑M1/1‑245 (MQ=255)                      gCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaact                                                                                                                                                                                                                 >  1:581924‑M1/1‑244 (MQ=255)                        gTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGttt                                                                                                                                                                                                                      >  1:624850/1‑241 (MQ=255)                          aTCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaact                                                                                                                                                                                                                 >  1:313499‑M1/1‑240 (MQ=255)                             ccttttatTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                       <  1:727216‑M1/249‑15 (MQ=255)                             cTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                       <  1:498708‑M1/251‑15 (MQ=255)                             cTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                       <  1:722471‑M1/251‑15 (MQ=255)                                    ttCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaa                                                                                                                                                                                                <  1:300441‑M1/251‑22 (MQ=255)                                             gAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTATTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTatctagttttaagatcatcaattatcgat                                                                                                                                                                                        <  1:376033‑M1/250‑30 (MQ=255)                                                   cccAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagt                                                                                                                                                                                 >  1:281989‑M1/1‑215 (MQ=255)                                                        aaaCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatct                                                                                                                                                                            <  1:136156‑M1/251‑42 (MQ=255)                                                          aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                          <  1:549450‑M1/251‑44 (MQ=255)                                                          aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                          <  1:215261‑M1/251‑44 (MQ=255)                                                          aCGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                          <  1:204509‑M1/251‑44 (MQ=255)                                                              cACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                      <  1:100361‑M1/251‑48 (MQ=255)                                                              cACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                      <  1:238080‑M1/251‑48 (MQ=255)                                                                cGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                      >  1:142546‑M1/1‑202 (MQ=255)                                                                 ggCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccc                                                                                                                                                                    >  1:848174‑M1/1‑201 (MQ=255)                                                                 ggCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTACCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatct                                                                                                                                                                            >  1:98597‑M1/1‑201 (MQ=255)                                                                      gCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatacccc                                                                                                                                                                   >  1:885011‑M1/1‑196 (MQ=255)                                                                       cAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagag                                                                                                                                                                                  <  1:477613‑M1/230‑36 (MQ=255)                                                                         gTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                            >  1:4241‑M1/1‑193 (MQ=255)                                                                         gTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaatt                                                                                                                                                                                              >  1:766993‑M1/1‑193 (MQ=255)                                                                          tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                          <  1:609080‑M1/251‑60 (MQ=255)                                                                          tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                          <  1:587990‑M1/251‑60 (MQ=255)                                                                          tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                            <  1:515339‑M1/249‑58 (MQ=255)                                                                          tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                            <  1:228092‑M1/249‑58 (MQ=255)                                                                          tGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTGTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataa                                                                                                                                                             <  1:438952‑M1/248‑57 (MQ=255)                                                                           gCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattca                                                                                                                                                         >  1:386138‑M1/1‑191 (MQ=255)                                                                           gCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagata                                                                                                                                                                       <  1:594226‑M1/237‑47 (MQ=255)                                                                                aatCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcata                                                                                                                                                    >  1:476571‑M1/1‑186 (MQ=255)                                                                                aatCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGT‑‑TAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaa                                                                                                                                                  >  1:241258‑M1/1‑184 (MQ=255)                                                                                       aaaaTAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaatta                                                                                                                                                                                             <  1:798024‑M1/203‑25 (MQ=255)                                                                                         aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagatac                                                                                                                                                                      <  1:600198‑M1/224‑48 (MQ=255)                                                                                         aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                          >  1:803188‑M1/1‑177 (MQ=255)                                                                                         aataaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                       >  1:78263‑M1/1‑177 (MQ=255)                                                                                           taaataGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaat                                                                                                                                         <  1:826185‑M1/251‑77 (MQ=255)                                                                                                  aTTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatt                                                                                                                                  <  1:523010‑M1/251‑84 (MQ=255)                                                                                                                  cgTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTATCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttt                                                                                                                                <  1:351355‑M1/237‑86 (MQ=255)                                                                                                                           aTTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattc                                                                                                                                                          >  1:91944‑M1/1‑143 (MQ=255)                                                                                                                                               aTAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagat                                                                                                                                                                                                      <  1:564690‑M1/138‑16 (MQ=255)                                                                                                                                                               ttGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacgg                                                                     <  1:277751‑M1/251‑145 (MQ=255)                                                                                                                                                                 gTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaaga                                                                                                                                                                                                       >  1:279010‑M1/1‑105 (MQ=255)                                                                                                                                                                                    gTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataat                                                                                                                                                            >  1:879531‑M1/1‑86 (MQ=255)                                                                                                                                                                                         gTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctag                                                                                                                                                                          <  1:892916‑M1/124‑44 (MQ=255)                                                                                                                                                                                               gTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatgttacggcaaatccttttcctaaaaagtaaattgtcagt                                     >  1:90798‑M1/1‑75 (MQ=255)                                                                                                                                                                                                         gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatccattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctgg                                                                                               >  1:148022‑M1/1‑65 (MQ=255)                                                                                                                                                                                                         gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaa                           >  1:37942‑M1/1‑65 (MQ=255)                                                                                                                                                                                                         gCCTCCTATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacgg                                                                     >  1:149953‑M1/1‑65 (MQ=255)                                                                                                                                                                                                          cctcctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaat                          <  1:138048‑M1/251‑188 (MQ=255)                                                                                                                                                                                                           ctcctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAg                                                                                                                                                                                                                                  <  1:82661/50‑1 (MQ=255)                                                                                                                                                                                                             cctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataat                       >  1:224522‑M1/1‑61 (MQ=255)                                                                                                                                                                                                             cctATCGTTCGTCTCTTGCCACATTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataat                       <  1:54768‑M1/251‑191 (MQ=255)                                                                                                                                                                                                                                  caTTTGGCGAAATGGTTGTGTTATAAGAAACCGGCGTTTTaactagttttaagatcatcaattatcgataaagagttatctagataccccccataattcatcataaatgccacaataatcatttttgtgacaaaatcataaattaatgctgttgctggagcttcaattttgccagatattacggcaaatccttttcctaaaaagtaaattgtcagtgtcatgccaataattgtaaaaagtgagctggcaaa  >  1:710751‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           TTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGGCAATTTTGCTGACTTCCGGTTGATAATCGGTTTCCGTTTTGTCATCCGTCGGCTGAATGGTTTTGTTATCGTTCATCTCTGCCTCCTATCGTTCGTCTCTTGCCACATTTGGTGAAATGGTCGTGTTATAAGTTACCGGTGTTTTTCCGTAACTTTGATTGTAAATGCCGACCACTTTTTCACCGCTACGTAATACCAATTTTTCTGCTGTTTTTTGGATGACTAATACCTGATAATTACCGTCTTGTTTAACATTAGTATTTAACAGACTTTCCGTGGAGCCTTCTAACAAGAAGGCTGCAGGAAAGGTTTTATTTGGGGCAAATCCAAGATAAGTAAAAATACCGTCATCATAA  >  1/1137654‑1138129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑