Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,137,562	A→C	I96M (ATT→ATG)	unknown ←	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,137,562	0	A	C	100.0%	220.2 / NA	76	I96M (ATT→ATG)	unknown	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/39);  total (37/39)

GTTTATGCGCTTTACCCGCTTTAAACGTACTGACGTTATCTTCAAACATATCGCCTTTTGCCGGTTCCGGTGGTTGATTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATTTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGGGGGGCGGATGGAATATCTAACGCCTCTGTCGTACTATGATTTTCTACTGGAGCGGTTGCGGTAGGAATTGGTTGTGGCTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGG  >  1/1137321‑1137773                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                    gTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTtgt                                                                                                                                                                                                            >  1:726828/1‑251 (MQ=255) gTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTtgg                                                                                                                                                                                                            >  1:565268/1‑250 (MQ=255)                  gCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTtg                                                                                                                                                                                                             <  1:642938/233‑1 (MQ=255)                  gCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAAt                                                                                                                                                                                           >  1:399249/1‑251 (MQ=255)                  gCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAAt                                                                                                                                                                                           >  1:762/1‑251 (MQ=255)                                        cTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCggtggg                                                                                                                                                                     >  1:186330/1‑250 (MQ=255)                                               tatatCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGt                                                                                                                                                                                                               >  1:593475/2‑202 (MQ=255)                                               tatatCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTaa                                                                                                                                                              >  1:264328/2‑251 (MQ=255)                                                  atCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATg                                                                                                                                                           <  1:484210/251‑1 (MQ=255)                                                          ttGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGaa                                                                                                                                                   <  1:848378/251‑1 (MQ=255)                                                                      gtggtTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACcgtcgt                                                                                                                                        <  1:395665/250‑1 (MQ=255)                                                                       tggttggtTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTAc                                                                                                                                      <  1:50322/251‑1 (MQ=255)                                                                          ttggttAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCg                                                                                                                                    <  1:892278/246‑1 (MQ=255)                                                                             gttAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGATACAATCCTTTGATTAAACGTGGGGCGGTTGGCATATCTAACGCCTCTGTCGTTCTATGCTTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGTGATTTCTTCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGCAtt                                                                                                                                >  1:592228/2‑251 (MQ=255)                                                                             gttAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAAtt                                                                                                                                >  1:132038/2‑251 (MQ=255)                                                                             gttAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGGGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAAt                                                                                                                                 >  1:537108/2‑250 (MQ=255)                                                                                        tgtCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTc                                                                                                                      >  1:117191/1‑250 (MQ=255)                                                                                             gTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTaa                                                                                                                 >  1:48056/1‑250 (MQ=255)                                                                                              ttGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:537536/251‑1 (MQ=255)                                                                                              ttGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:519502/251‑1 (MQ=255)                                                                                              ttGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:712206/251‑1 (MQ=255)                                                                                              ttGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:366638/251‑1 (MQ=255)                                                                                                 tATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGttttgaa                                                                                                                                                    >  1:605665/1‑208 (MQ=255)                                                                                                   tCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               >  1:829485/1‑246 (MQ=255)                                                                                                   tCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCtgtg                                                                                                           >  1:467966/1‑250 (MQ=255)                                                                                                   tCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCtgtg                                                                                                           >  1:88770/1‑250 (MQ=255)                                                                                                        aTTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGtt                                                                                                     >  1:494765/1‑251 (MQ=255)                                                                                                             gAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTg                                                                                                    <  1:106446/247‑1 (MQ=255)                                                                                                             gAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTa                                                                                                >  1:477622/1‑251 (MQ=255)                                                                                                                catctGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATc                                                                                              >  1:770005/5‑250 (MQ=255)                                                                                                                catctGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGGAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCg                                                                                                                                    >  1:217931/5‑212 (MQ=255)                                                                                                                          ggCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATg                                                                                                                                                           <  1:529468/179‑1 (MQ=255)                                                                                                                           gCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTggt                                                                                                                                                                                                         <  1:714805/132‑2 (MQ=255)                                                                                                                           gCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTc                                                                                                                      <  1:46622/215‑1 (MQ=255)                                                                                                                           gCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCaa                                                                                  >  1:161281/1‑251 (MQ=255)                                                                                                                               gTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCt                                                                                                              <  1:660966/219‑1 (MQ=255)                                                                                                                                 aaCGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGTGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGa                                                                            <  1:415863/251‑1 (MQ=255)                                                                                                                                        cGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAAccc                                                                     <  1:204104/251‑1 (MQ=255)                                                                                                                                              cGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCaaaaa                                                                >  1:845213/1‑250 (MQ=255)                                                                                                                                                aTAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTgg                                                                                                                                                                                                                <  1:575630/104‑1 (MQ=255)                                                                                                                                                aTAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATCGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGc                                                                                                          >  1:791342/1‑206 (MQ=255)                                                                                                                                                   gTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGcc                                                                                                         <  1:868652/204‑1 (MQ=255)                                                                                                                                                      cccGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:66401/195‑1 (MQ=255)                                                                                                                                                           agaCAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCtt                                                                                           >  1:487867/1‑210 (MQ=255)                                                                                                                                                           agaCAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAAGCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGc                                                   <  1:737058/250‑1 (MQ=255)                                                                                                                                                             aCAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAg                                                 <  1:51334/250‑1 (MQ=255)                                                                                                                                                                  ccTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaat                                           >  1:812013/1‑251 (MQ=255)                                                                                                                                                                    tttGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaataa                                         <  1:763379/251‑1 (MQ=255)                                                                                                                                                                      tGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATc                                       <  1:664779/251‑1 (MQ=255)                                                                                                                                                                       gATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGcc                                                            >  1:579407/1‑229 (MQ=255)                                                                                                                                                                            aaCGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGaaaa                                 >  1:571736/1‑251 (MQ=255)                                                                                                                                                                             acgtggggCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:481830/168‑1 (MQ=255)                                                                                                                                                                             acgtggggCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCAGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:232463/168‑1 (MQ=255)                                                                                                                                                                              cgtggggCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATcc                                                                                             <  1:715761/186‑1 (MQ=255)                                                                                                                                                                                  gggCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaataa                                         >  1:660980/1‑237 (MQ=255)                                                                                                                                                                                     cGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGa                        <  1:53165/251‑1 (MQ=255)                                                                                                                                                                                       gATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGAt                       <  1:188800/250‑1 (MQ=255)                                                                                                                                                                                       gATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGAt                       <  1:853096/250‑1 (MQ=255)                                                                                                                                                                                         tggcatatCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAAc                                                               <  1:774243/204‑1 (MQ=255)                                                                                                                                                                                         tggcatatCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaataa                                         >  1:815096/5‑230 (MQ=255)                                                                                                                                                                                          ggcatatCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAAt                                                                          <  1:878165/193‑1 (MQ=255)                                                                                                                                                                                          ggcatatCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTg                   >  1:61490/4‑251 (MQ=255)                                                                                                                                                                                             atatCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGtt                <  1:178901/251‑1 (MQ=255)                                                                                                                                                                                               atCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaataa                                         <  1:518547/224‑1 (MQ=255)                                                                                                                                                                                               atCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGtttt              >  1:648330/1‑251 (MQ=255)                                                                                                                                                                                               atCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGtttt              >  1:830719/1‑251 (MQ=255)                                                                                                                                                                                                tCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAAccc                                                                     <  1:721287/195‑1 (MQ=255)                                                                                                                                                                                                tCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGtt                <  1:887704/248‑1 (MQ=255)                                                                                                                                                                                                     cGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACcg         >  1:717041/1‑250 (MQ=255)                                                                                                                                                                                                       ccTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGc                                                                                                          <  1:811604/151‑1 (MQ=255)                                                                                                                                                                                                       ccTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAAccc                                                                     >  1:159999/1‑188 (MQ=255)                                                                                                                                                                                                       ccTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGAGCaa                                            >  1:147723/1‑213 (MQ=255)                                                                                                                                                                                                            gTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTgg  >  1:822487/1‑250 (MQ=255)                                                                                                                                                                                                                ttcTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGc                                                                                                                                                <  1:676221/102‑1 (MQ=255)                                                                                                                                                                                                                            cTACTGGAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCaa                                            >  1:241651/1‑192 (MQ=255)                                                                                                                                                                                                                                 ggAGCGGTTGCGGTAGGCATTGGTTGTGGTTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAAcc                                                                                                               <  1:114553/120‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                    GTTTATGCGCTTTACCCGCTTTAAACGTACTGACGTTATCTTCAAACATATCGCCTTTTGCCGGTTCCGGTGGTTGATTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATTTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGGGGGGCGGATGGAATATCTAACGCCTCTGTCGTACTATGATTTTCTACTGGAGCGGTTGCGGTAGGAATTGGTTGTGGCTCCGGATTTAAAATCGCCGGGATTTCTGCCGGTGGTGCCGTAAATGTTTTTGAACGCACCGTCGTACCGAATTCTTCTTTTTCCTTAACCTGTGCCGGTTGCGTATCCTTTTTATTCAAAAAGGAATAACCCAAAAACGCCACGGCAAGCAGTGCAATAATCAGAAAAATAAATAGATTTTGGTTTTTACCGCGTTTGG  >  1/1137321‑1137773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑