Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,137,337	C→T	A171A (GCG→GCA)	unknown ←	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,137,337	0	C	T	100.0%	188.8 / NA	71	A171A (GCG→GCA)	unknown	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/41);  total (30/41)

TTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAGAGCCTTTTTCAATCAGTAGTACCGTGCCGTTTGCACTATATACATCGTTTGCCACAACGCAGCCGAGATTGCCGGCCACGGTGGAAACAAGTTTTGTGCGTAAGGAACACTGAATAAAAGTGCCTTTTGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGCGCTTTACCCGCTTTAAACGTACTGACGTTATCTTCAAACATATCGCCTTTTGCCGGTTCCGGTGGTTGATTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATTTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGGGGGGCGGATGGAATATCTAACGCCTCTGTCGTACTATGATTTTCTACTGGAGCGGTTGCGGTAGGAATTGGTTGT  >  1/1137118‑1137571                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           aatccccctggttcttataaagcgtaggcggaatattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTtcaaata                                                                                                                                                                                                             <  1:534921‑M2/76‑3 (MQ=255) aatccccctggttcttataaagcgtaggcggaatattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTtcaaata                                                                                                                                                                                                             <  1:657763‑M2/76‑3 (MQ=255)          ggttcttataaagcgtaggcggaatattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCtt                                                                                                                                                                                                    >  1:465062‑M2/167‑251 (MQ=255)              cttataaagcgtaggcggaatattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGc                                                                                                                                                                                                <  1:397013‑M2/89‑1 (MQ=255)                               gaatattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGttggt                                                                                                                                                                               >  1:556672‑M2/146‑249 (MQ=255)                                  tattaatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTcaaat                                                                                                                                                                                                              >  1:895880‑M2/143‑216 (MQ=255)                                      aatggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTAc                                                                                                                                                                        <  1:11000‑M2/113‑1 (MQ=255)                                         ggttttctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCgt                                                                                                                                                                     >  1:723875‑M2/136‑251 (MQ=255)                                              tctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAg                                                                                                                                                                <  1:100574‑M2/121‑1 (MQ=255)                                               ctccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGt                                                                                                                                                               >  1:649342‑M2/130‑251 (MQ=255)                                                 ccaggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTg                                                                                                                                                             >  1:445681‑M2/128‑251 (MQ=255)                                                    ggatactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTAt                                                                                                                                                          >  1:702748‑M2/125‑251 (MQ=255)                                                      atactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATc                                                                                                                                                         <  1:119187‑M2/128‑1 (MQ=255)                                                      atactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATc                                                                                                                                                         <  1:344739‑M2/128‑1 (MQ=255)                                                      atactttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCt                                                                                                                                                        <  1:389129‑M2/129‑1 (MQ=255)                                                        actttgtgcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTAt                                                                                                                                                          >  1:312015‑M2/121‑247 (MQ=255)                                                               gcaatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGa                                                                                                                                               >  1:659247‑M2/114‑251 (MQ=255)                                                                caatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGaa                                                                                                                                              <  1:257774‑M2/139‑1 (MQ=255)                                                                caatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGaa                                                                                                                                              <  1:707011‑M2/139‑1 (MQ=255)                                                                caatttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGa                                                                                                                                               <  1:223166‑M2/138‑1 (MQ=255)                                                                   tttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACAt                                                                                                                                           >  1:861862‑M2/110‑251 (MQ=255)                                                                    ttcagagccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACatc                                                                                                                                          <  1:233769‑M2/143‑2 (MQ=255)                                                                           ccggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGaaa                                                                                                                                     <  1:564717‑M2/148‑1 (MQ=255)                                                                             ggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTg                                                                                                                                                             <  1:205200‑M2/124‑1 (MQ=255)                                                                             ggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAAc                                                                                                                                    <  1:199476‑M2/149‑1 (MQ=255)                                                                             ggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACgg                                                                                                                                  >  1:669835‑M2/100‑250 (MQ=255)                                                                             ggcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACgg                                                                                                                                  >  1:846658‑M2/100‑250 (MQ=255)                                                                              gcttgtaccgtatcagtcggattaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACgg                                                                                                                                  >  1:14698‑M2/99‑249 (MQ=255)                                                                                                  attaaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGAt                                                                                                            <  1:461564‑M2/173‑1 (MQ=255)                                                                                                     aaatgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGt                                                                                                         >  1:312825‑M2/76‑251 (MQ=255)                                                                                                       atgtcccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAATGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTAc                                                                                                       <  1:204564‑M2/178‑1 (MQ=255)                                                                                                           cccccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGt                                                                                                                                                               <  1:299954‑M2/122‑1 (MQ=255)                                                                                                             cccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGgag                                                                                                 <  1:443178‑M2/184‑1 (MQ=255)                                                                                                              ccgtttggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGgaga                                                                                                >  1:31311‑M2/67‑251 (MQ=255)                                                                                                                    ggctaattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATc                                                                                                                                                         >  1:611436‑M2/61‑188 (MQ=255)                                                                                                                        aattgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTg                                                                                                                                                       >  1:755700‑M2/57‑186 (MQ=255)                                                                                                                           tgagtagataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCg                                                                                                                                                                      >  1:825214‑M2/54‑168 (MQ=255)                                                                                                                                agataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGCCGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTAc                                                                                                                                                                        >  1:386557‑M2/49‑161 (MQ=255)                                                                                                                                agataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTAc                                                                                                       >  1:95516‑M2/49‑226 (MQ=255)                                                                                                                                 gataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGAc                                                                                               <  1:317537‑M2/186‑1 (MQ=255)                                                                                                                                  ataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTAc                                                                                                                                                                        <  1:798201‑M2/113‑1 (MQ=255)                                                                                                                                  ataatgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGt                                                                                                         <  1:628103‑M2/176‑1 (MQ=255)                                                                                                                                      tgcactggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGc                                                                        <  1:421122‑M2/209‑1 (MQ=255)                                                                                                                                            ggttgaatcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACg                                                                              <  1:221399‑M2/203‑1 (MQ=255)                                                                                                                                                  atcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATc                                                            <  1:465065‑M2/221‑1 (MQ=255)                                                                                                                                                  atcggtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATc                                                            <  1:762157‑M2/221‑1 (MQ=255)                                                                                                                                                      gtgatcatgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTaa                                                         >  1:53515‑M2/27‑250 (MQ=255)                                                                                                                                                             tgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTaa                                                         <  1:340545‑M2/224‑1 (MQ=255)                                                                                                                                                             tgcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCct                                                    >  1:735888‑M2/20‑248 (MQ=255)                                                                                                                                                              gcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACg                                                                              <  1:226926‑M2/203‑1 (MQ=255)                                                                                                                                                              gcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCCTTGATTAAACg                                                                              >  1:851247‑M2/19‑221 (MQ=255)                                                                                                                                                              gcttaacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTAGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACatc                                                                                                                                          >  1:84462‑M2/19‑160 (MQ=255)                                                                                                                                                                 taacatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTg                                                                                                                                                             >  1:80894‑M2/16‑139 (MQ=255)                                                                                                                                                                   acatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCgtt                                            >  1:824851‑M2/14‑249 (MQ=255)                                                                                                                                                                   acatgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGGTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGt                                                <  1:438377‑M2/233‑1 (MQ=255)                                                                                                                                                                    catgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTAc                                                                                                                                                                        <  1:505888‑M2/113‑1 (MQ=255)                                                                                                                                                                    catgatggcattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGgag                                                                                                 <  1:119367‑M2/184‑1 (MQ=255)                                                                                                                                                                      tgatggcattGCCAACAGTAAATTGGCATTGAACGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCt                                                                                                                                                                                                     <  1:45897‑M2/84‑1 (MQ=255)                                                                                                                                                                         tggcaatGCCAACAGTAAAGTGGAATTGACCGGGGGTTTATGGGCTTTACCTGCTTTAAACCTACTGGCGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGGATTCTGAACATCTGAAACGGCTTGTAACGTTTCGGCATCGGTAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGAt                                     <  1:711814/245‑1 (MQ=255)                                                                                                                                                                            cattGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGAttt                                   <  1:572534‑M2/246‑1 (MQ=255)                                                                                                                                                                             attGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGAtttt                                  >  1:436239‑M2/4‑250 (MQ=255)                                                                                                                                                                                 ccAACAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTACAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTAc                              <  1:93970/250‑1 (MQ=255)                                                                                                                                                                                     cAGTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACg                                                                              <  1:118425/198‑1 (MQ=255)                                                                                                                                                                                       gTAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGGACCGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGGTTCGCCATCGATAGTACCCGGAGACAATCCATTGATTAAACGTGGGGCGGATGGCATATCTAACGcc                                                     <  1:427208/221‑1 (MQ=255)                                                                                                                                                                                        tAAATTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAg                         <  1:850764/248‑1 (MQ=255)                                                                                                                                                                                         aaaTTGGCATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGa                                      <  1:60336/234‑1 (MQ=255)                                                                                                                                                                                                cATTGACCGGGAGTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGt               <  1:875313/250‑1 (MQ=255)                                                                                                                                                                                                            gTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTtgt  >  1:726828/1‑251 (MQ=255)                                                                                                                                                                                                            gTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAACGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTGCGGTAGGCATTGGTtgg  >  1:565268/1‑250 (MQ=255)                                                                                                                                                                                                            gTTTATGGGCTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGTGGGGCGGATGGCATATCTAAAGCCTCTGTCGTTCTATGATTTTCTACTGGAGCGGTTTCGGTAggc           >  1:46284/1‑241 (MQ=255)                                                                                                                                                                                                                    gcTTTACCTGCTTTAAACGTACTGACGTTATCTTCAAATATATCGCCTTTTGCCGGTTCCGGTGGTTGGTTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATCTGAAACGGCTTGTAACGTTTCGCCATCGATAGTAcc                                                                                                      <  1:833901/143‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           TTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAGAGCCTTTTTCAATCAGTAGTACCGTGCCGTTTGCACTATATACATCGTTTGCCACAACGCAGCCGAGATTGCCGGCCACGGTGGAAACAAGTTTTGTGCGTAAGGAACACTGAATAAAAGTGCCTTTTGCCAACAGTAAATTGGCATTGACCGGGAGTTTATGCGCTTTACCCGCTTTAAACGTACTGACGTTATCTTCAAACATATCGCCTTTTGCCGGTTCCGGTGGTTGATTAGTTACCGTGTCAGTTGTATCTGTATTCTGAACATTTGAAACGGCTTGTAACGTTTCGCCATCGATAGTACCCGGAGACAATCCTTTGATTAAACGGGGGGCGGATGGAATATCTAACGCCTCTGTCGTACTATGATTTTCTACTGGAGCGGTTGCGGTAGGAATTGGTTGT  >  1/1137118‑1137571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑