Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,937	G→A	L305L (CTA→TTA)	unknown ←	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,937	0	G	A	100.0%	187.2 / NA	69	L305L (CTA→TTA)	unknown	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/32);  total (37/32)

TAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAGA  >  1/1136692‑1137165                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                 <  1:379915/249‑1 (MQ=255) tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                 <  1:271678/249‑1 (MQ=255)    aaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTgg                                                                                                                                                                                                                              >  1:849504/1‑251 (MQ=255)     aTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGt                                                                                                                                                                                                                             >  1:367161/1‑251 (MQ=255)      ttGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGtt                                                                                                                                                                                                                            >  1:393779/1‑251 (MQ=255)              tCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGt                                                                                                                                                                                                                    >  1:711969/1‑251 (MQ=255)                     aaGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATg                                                                                                                                                                                                             <  1:654303/251‑1 (MQ=255)                        agTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCt                                                                                                                                                                                                           <  1:221211/250‑1 (MQ=255)                         gTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                 >  1:201633/1‑227 (MQ=255)                           ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                                                                       <  1:103920/239‑1 (MQ=255)                           ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                                                                       <  1:468242/239‑1 (MQ=255)                                 ccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                                                                  <  1:505424/244‑1 (MQ=255)                                     ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                                                             >  1:212741‑M1/3‑251 (MQ=255)                                     ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                                                             >  1:514503‑M1/3‑251 (MQ=255)                                     ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACat                                                                                                                                                                                                     >  1:196232/3‑243 (MQ=255)                                     ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAATCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAt                                                                                                                                                                                             >  1:456171/3‑251 (MQ=255)                                      gtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGc                                                                                                                                                                                                            >  1:4890/2‑235 (MQ=255)                                               tcctATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagt                                                                                                                                                                                   >  1:248387‑M1/1‑242 (MQ=255)                                                                ttCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgacc                                                                                                                                                                    >  1:605604‑M1/1‑225 (MQ=255)                                                                 tCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccggg                                                                                                                                                                 >  1:288050‑M1/1‑224 (MQ=255)                                                                        caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                                                                          <  1:482138‑M1/251‑35 (MQ=255)                                                                        caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                                                                          <  1:508186‑M1/251‑35 (MQ=255)                                                                            gCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatggg                                                                                                                                                       >  1:291545‑M1/1‑213 (MQ=255)                                                                                 aaTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggcttta                                                                                                                                                  >  1:55230‑M1/1‑208 (MQ=255)                                                                                  aTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacc                                                                                                                                                <  1:558750‑M1/251‑45 (MQ=255)                                                                                   tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                                                                  <  1:157540/200‑1 (MQ=255)                                                                                   tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttac                                                                                                                                                 >  1:877090‑M1/1‑206 (MQ=255)                                                                                                                  aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                                                                <  1:657763‑M1/251‑77 (MQ=255)                                                                                                                  aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                                                                <  1:534921‑M1/251‑77 (MQ=255)                                                                                                                           ggTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgcctt                                                                                                       >  1:465062‑M1/1‑166 (MQ=255)                                                                                                                               cTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgc                                                                                                   <  1:397013‑M1/251‑90 (MQ=255)                                                                                                                                       gCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAttgccaacagt                                                                                                                                                                                   <  1:374985/163‑10 (MQ=255)                                                                                                                                                gAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggt                                                                                  >  1:556672‑M1/1‑145 (MQ=255)                                                                                                                                                   tatTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaat                                                                                                                 >  1:895880‑M1/1‑142 (MQ=255)                                                                                                                                                       aaTGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttac                                                                           <  1:11000‑M1/251‑114 (MQ=255)                                                                                                                                                          ggTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgt                                                                        >  1:723875‑M1/1‑135 (MQ=255)                                                                                                                                                               tctccaggatactttGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcag                                                                   <  1:100574‑M1/244‑122 (MQ=255)                                                                                                                                                                ctccaggatacttTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagt                                                                  >  1:649342‑M1/7‑129 (MQ=255)                                                                                                                                                                  ccaggatacTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttg                                                                >  1:445681‑M1/5‑127 (MQ=255)                                                                                                                                                                     ggaTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                                                             >  1:702748‑M1/2‑124 (MQ=255)                                                                                                                                                                       aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatct                                                           <  1:389129‑M1/251‑130 (MQ=255)                                                                                                                                                                       aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                                                            <  1:119187‑M1/250‑129 (MQ=255)                                                                                                                                                                       aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                                                            <  1:344739‑M1/250‑129 (MQ=255)                                                                                                                                                                         aCTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                                                             >  1:312015‑M1/1‑120 (MQ=255)                                                                                                                                                                                gCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga                                                  >  1:659247‑M1/1‑113 (MQ=255)                                                                                                                                                                                 cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa                                                 <  1:707011‑M1/251‑140 (MQ=255)                                                                                                                                                                                 cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa                                                 <  1:257774‑M1/251‑140 (MQ=255)                                                                                                                                                                                 cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga                                                  <  1:223166‑M1/250‑139 (MQ=255)                                                                                                                                                                                    tttCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacat                                              >  1:861862‑M1/1‑109 (MQ=255)                                                                                                                                                                                     ttcagagccGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatc                                             <  1:233769‑M1/247‑144 (MQ=255)                                                                                                                                                                                            ccGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaa                                        <  1:564717‑M1/249‑149 (MQ=255)                                                                                                                                                                                              ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                     >  1:669835‑M1/1‑99 (MQ=255)                                                                                                                                                                                              ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                     >  1:846658‑M1/1‑99 (MQ=255)                                                                                                                                                                                              ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaac                                       <  1:199476‑M1/248‑150 (MQ=255)                                                                                                                                                                                              ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttg                                                                <  1:205200‑M1/223‑125 (MQ=255)                                                                                                                                                                                               gCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                     >  1:14698‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                   attaaatgtcccccgtttggctaattgaGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgat               <  1:461564‑M1/238‑174 (MQ=255)                                                                                                                                                                                                                      aaatgtcccccgtttggctaatTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagt            >  1:312825‑M1/11‑75 (MQ=255)                                                                                                                                                                                                                        atgtcccccgtttggctaATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaatgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtac          <  1:204564‑M1/243‑179 (MQ=255)                                                                                                                                                                                                                            cccccgtttgGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagt                                                                  <  1:299954‑M1/187‑123 (MQ=255)                                                                                                                                                                                                                              cccgttTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtacccggag    <  1:443178‑M1/249‑185 (MQ=255)                                                                                                                                                                                                                               ccgtTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtacccggaga   >  1:31311‑M1/2‑66 (MQ=255)                                                                                                                                                                                                                                     ggcTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                                                            >  1:611436‑M1/2‑60 (MQ=255)                                                                                                                                                                                                                                         aaTTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctg                                                          >  1:755700‑M1/1‑56 (MQ=255)                                                                                                                                                                                                                                            tgagtagATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccg                                                                         >  1:825214‑M1/4‑53 (MQ=255)                                                                                                                                                                                                                                                 aGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgccgttatcttcaaatatatcgccttttgccggttccggtggttggttagttac                                                                           >  1:386557‑M1/1‑48 (MQ=255)                                                                                                                                                                                                                                                 aGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtac          >  1:95516‑M1/1‑48 (MQ=255)                                                                                                                                                                                                                                                  gATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtacccggagac  <  1:317537‑M1/233‑187 (MQ=255)                                                                                                                                                                                                                                                  gATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggc                                                                                                                                                                           >  1:624423‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     TAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAGA  >  1/1136692‑1137165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑