Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,920	T→G	A310A (GCA→GCC)	unknown ←	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,920	0	T	G	100.0%	168.3 / NA	61	A310A (GCA→GCC)	unknown	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/28);  total (33/28)

AAGTAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAG  >  1/1136689‑1137164                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gagtgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATaatg                                                                                                                                                                                                                                   >  1:83435/6‑248 (MQ=255)    tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                <  1:379915/249‑1 (MQ=255)    tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                <  1:271678/249‑1 (MQ=255)       aaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTgg                                                                                                                                                                                                                             >  1:849504/1‑251 (MQ=255)        aTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGt                                                                                                                                                                                                                            >  1:367161/1‑251 (MQ=255)         ttGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGtt                                                                                                                                                                                                                           >  1:393779/1‑251 (MQ=255)                 tCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGt                                                                                                                                                                                                                   >  1:711969/1‑251 (MQ=255)                        aaGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATg                                                                                                                                                                                                            <  1:654303/251‑1 (MQ=255)                           agTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCt                                                                                                                                                                                                          <  1:221211/250‑1 (MQ=255)                            gTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                                                                >  1:201633/1‑227 (MQ=255)                              ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                                                                      <  1:103920/239‑1 (MQ=255)                              ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                                                                      <  1:468242/239‑1 (MQ=255)                                    ccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                                                                 <  1:505424/244‑1 (MQ=255)                                        ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                                                            >  1:212741‑M1/3‑251 (MQ=255)                                        ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                                                            >  1:514503‑M1/3‑251 (MQ=255)                                        ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACat                                                                                                                                                                                                    >  1:196232/3‑243 (MQ=255)                                        ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAATCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAt                                                                                                                                                                                            >  1:456171/3‑251 (MQ=255)                                         gtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGc                                                                                                                                                                                                           >  1:4890/2‑235 (MQ=255)                                                  tcctATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagt                                                                                                                                                                                  >  1:248387‑M1/1‑242 (MQ=255)                                                                  aTTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATaatg                                                                                                                                                                                                                                   >  1:509628/1‑183 (MQ=255)                                                                   ttCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgacc                                                                                                                                                                   >  1:605604‑M1/1‑225 (MQ=255)                                                                    tCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccggg                                                                                                                                                                >  1:288050‑M1/1‑224 (MQ=255)                                                                        atacaTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGAt                                                                                                                                                                                                                                       >  1:153891/3‑176 (MQ=255)                                                                           caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                                                                         <  1:482138‑M1/251‑35 (MQ=255)                                                                           caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                                                                         <  1:508186‑M1/251‑35 (MQ=255)                                                                               gCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatggg                                                                                                                                                      >  1:291545‑M1/1‑213 (MQ=255)                                                                                    aaTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggcttta                                                                                                                                                 >  1:55230‑M1/1‑208 (MQ=255)                                                                                     aTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacc                                                                                                                                               <  1:558750‑M1/251‑45 (MQ=255)                                                                                      tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                                                                 <  1:157540/200‑1 (MQ=255)                                                                                      tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttac                                                                                                                                                >  1:877090‑M1/1‑206 (MQ=255)                                                                                                                     aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                                                               <  1:657763‑M1/251‑77 (MQ=255)                                                                                                                     aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                                                               <  1:534921‑M1/251‑77 (MQ=255)                                                                                                                              ggTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgcctt                                                                                                      >  1:465062‑M1/1‑166 (MQ=255)                                                                                                                                  cTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgc                                                                                                  <  1:397013‑M1/251‑90 (MQ=255)                                                                                                                                          gCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAttgccaacagt                                                                                                                                                                                  <  1:374985/163‑10 (MQ=255)                                                                                                                                                   gAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggt                                                                                 >  1:556672‑M1/1‑145 (MQ=255)                                                                                                                                                      tatTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaat                                                                                                                >  1:895880‑M1/1‑142 (MQ=255)                                                                                                                                                          aaTGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttac                                                                          <  1:11000‑M1/251‑114 (MQ=255)                                                                                                                                                             ggTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgt                                                                       >  1:723875‑M1/1‑135 (MQ=255)                                                                                                                                                                  tctccaggatactttGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcag                                                                  <  1:100574‑M1/244‑122 (MQ=255)                                                                                                                                                                   ctccaggatacttTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagt                                                                 >  1:649342‑M1/7‑129 (MQ=255)                                                                                                                                                                     ccaggatacTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttg                                                               >  1:445681‑M1/5‑127 (MQ=255)                                                                                                                                                                        ggaTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                                                            >  1:702748‑M1/2‑124 (MQ=255)                                                                                                                                                                          aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatct                                                          <  1:389129‑M1/251‑130 (MQ=255)                                                                                                                                                                          aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                                                           <  1:119187‑M1/250‑129 (MQ=255)                                                                                                                                                                          aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                                                           <  1:344739‑M1/250‑129 (MQ=255)                                                                                                                                                                            aCTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                                                            >  1:312015‑M1/1‑120 (MQ=255)                                                                                                                                                                                   gCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga                                                 >  1:659247‑M1/1‑113 (MQ=255)                                                                                                                                                                                    cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa                                                <  1:257774‑M1/251‑140 (MQ=255)                                                                                                                                                                                    cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa                                                <  1:707011‑M1/251‑140 (MQ=255)                                                                                                                                                                                    cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga                                                 <  1:223166‑M1/250‑139 (MQ=255)                                                                                                                                                                                       tttCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacat                                             >  1:861862‑M1/1‑109 (MQ=255)                                                                                                                                                                                        ttcagagccGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatc                                            <  1:233769‑M1/247‑144 (MQ=255)                                                                                                                                                                                               ccGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaa                                       <  1:564717‑M1/249‑149 (MQ=255)                                                                                                                                                                                                 ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                    >  1:846658‑M1/1‑99 (MQ=255)                                                                                                                                                                                                 ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                    >  1:669835‑M1/1‑99 (MQ=255)                                                                                                                                                                                                 ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaac                                      <  1:199476‑M1/248‑150 (MQ=255)                                                                                                                                                                                                 ggCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttg                                                               <  1:205200‑M1/223‑125 (MQ=255)                                                                                                                                                                                                  gCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacgg                                    >  1:14698‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                      attaaatgtcccccgtttggctaattgaGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgat              <  1:461564‑M1/238‑174 (MQ=255)                                                                                                                                                                                                                         aaatgtcccccgtttggctaatTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagt           >  1:312825‑M1/11‑75 (MQ=255)                                                                                                                                                                                                                           atgtcccccgtttggctaATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaatgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtac         <  1:204564‑M1/243‑179 (MQ=255)                                                                                                                                                                                                                               cccccgtttgGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagt                                                                 <  1:299954‑M1/187‑123 (MQ=255)                                                                                                                                                                                                                                 cccgttTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtacccggag   <  1:443178‑M1/249‑185 (MQ=255)                                                                                                                                                                                                                                  ccgtTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacatctgaaacggcttgtaacgtttcgccatcgatagtacccggaga  >  1:31311‑M1/2‑66 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AAGTAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCGCCTTGCTGAATCTGACCGCTACGGAACTCACCGAACACGGTAG  >  1/1136689‑1137164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑