Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	143,328	C→T	A32V (GCG→GTG)	unknown →	D‑glycerate transporter (predicted)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	143,328	0	C	T	100.0%	161.1 / NA	60	A32V (GCG→GTG)	unknown	D‑glycerate transporter (predicted)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/26);  total (34/26)

AAGATTTTTGTATTTTTATATAAAGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGCGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAACAGCTGTTGGCG  >  1/143140‑143573                                                                                                                                                                                               |                                                                                                                                                                                                                                                                aaGATTTTTGTATTTTTATATAAAGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAACAA‑‑GCTGACGACGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGa                                                                                                                                                                                                   <  1:336832/251‑1 (MQ=255)     tttttGTATTTTTATATAAAGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑GCAAAAGCCGGCATACGGGATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCg                                                                                                                                                                                                               <  1:448977/235‑1 (MQ=255)             tttttATATAAAGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGGTTGGGCGGCGGTGCCGATTTAGGGCAAACCGTGAGGCTCATGATCGGCGGTgcg                                                                                                                                                                                        >  1:131286/1‑250 (MQ=255)                       aaGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGc                                                                                                                                                                                                                     >  1:129039/1‑211 (MQ=255)                                         gggCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGACATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAACA‑‑AGTATCGCCGGCATACGGCATGCTGGTGGGGGCGTTACTCGGCGGTTTGGTCGGCGGCTCCGAATTCGTGCAAGCCGTGAGTCTCATGATCGGCGGGGCGCAGGGcctgtctcttatccccatctccca                                                                                                                                                           >  1:394926/1‑228 (MQ=255)                                              aataatCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGGGGGGCCGATTTAGTGCAAACCGAGAGGCTCATGATTGGGGGGGGGCAGGGCATTACCACGGCGGTCAttggggtttttg                                                                                                                                                      >  1:123293/1‑240 (MQ=255)                                                taatCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGTGATCCTCTTGATTTTGAACAAGCAGAA‑‑GCCGGCATACGGGATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTAc                                                                                                                                                                             <  1:876707/226‑1 (MQ=255)                                                   tctcCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGtc                                                                                                                                                                                                         <  1:686423/195‑1 (MQ=255)                                                               tATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGGATTACCACGGCGGGCATGC‑GGGTTTTTGCCGCCCGTGTGTTtgcg                                                                                                                                    >  1:101076/1‑251 (MQ=255)                                                               tATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCAcgg                                                                                                                                                                        <  1:558029/216‑1 (MQ=255)                                                               tATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGGCTCATGATCGGCGGTGCGCAGGGCATTTCCACGGCGGGCCTGC‑GGATGTTTGTCGCCCGTGTGTTtgcg                                                                                                                                    >  1:149646/1‑251 (MQ=255)                                                                aTTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGGCGGCGGTGCCGATTTAGTGCCAACCGTGAGTCGCATGATCGGCGGGGGGCAGGGCATTAc                                                                                                                                                                             >  1:14513/1‑210 (MQ=255)                                                                  ttACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGGGGAGGGCATTACCACGGCGGTCACCA‑GGGTTTTAgccgcccggggggttgtgggg                                                                                                                                 >  1:508431/1‑236 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGGGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAgc                                                                                                                                                    <  1:135410/225‑1 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTTGTGCAAACCGCGAGTCTCATGATCCGCGGTGCGCAGGGCACTATCACGGCGGCCACGC‑GGGTTTTTGCCCCCGGGGTGTTGGCCGGCGGGCt‑g                                                                                                                          >  1:463195/1‑250 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATcggcgg                                                                                                                                                                                            >  1:74629/1‑186 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCCGGGCATTACCACCGCGGGCATGCGGGATTTT‑gccgcccggggggtggggggcgggctgg                                                                                                                          >  1:74394/1‑229 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGc                                                                                                                                                                                  <  1:15927/196‑1 (MQ=255)                                                                         gTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGGCGGCGGTGCCGATTTAGTGCAAACCGGGAGTCTCCTGATCGGCGGTGGCCAGGGCATTCCCACGGCGGTCCTGC‑GGATTTTAGCCGCCGGCGGGTTGGCGGGCGTGcgtg                                                                                                                          >  1:748757/1‑248 (MQ=255)                                                                         gTTCAACACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGtc                                                                                                                                                                                                         >  1:800071/1‑173 (MQ=255)                                                                           tcatcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGGCCTGC‑GGGTTTTTGCCGCCCGGGTGTTggcggggggggtggtt                                                                                                                        >  1:267786/1‑241 (MQ=255)                                                                            catcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCGAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCAcggcgg                                                                                                                                                                     >  1:125656/1‑206 (MQ=255)                                                                              tcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCa                                                                                                                                                                           >  1:77893/1‑198 (MQ=255)                                                                              tcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGGGCGCAGGGCCTTACCACGGCGGTCATGC‑GGGTTTTAACCGCCCGGGTGGTggcgggcgcgccggtttgt                                                                                                                     >  1:528538/1‑240 (MQ=255)                                                                              tcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGGCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGGGGGCAGGGCATTACCAcgg                                                                                                                                                                        >  1:680452/1‑201 (MQ=255)                                                                              tcaCAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGGCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCCCATGATCGGCGGGGCGCAGGGCATTACCCCGGCGGGCCTGC‑GGGTTTTTGCCGCCGGTGTGTTTGCGGGGGTGCt‑ggttga‑t                                                                                                                   >  1:283429/1‑251 (MQ=255)                                                                                   gaggagAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGCTTTAGTGCAAACCCTGAGTCTCATGGTCGGCTGGGCGCAGGGCATTACCACGGCGGTCATGC‑GGGTTTTTGCCGCCCGCGTGttctcgcgcgtgcgtattgtttcccg                                                                                                                >  1:635038/1‑227 (MQ=255)                                                                                     ggagAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGGGCCGAttt                                                                                                                                                                                                                          >  1:757509/1‑144 (MQ=255)                                                                                        gaATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCCTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCCCCGGGGTGTTggggggcggggcggttttgtcttttttaa                                                                                                           >  1:723055/1‑230 (MQ=255)                                                                                        gaATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATCTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGGGCCGGTTTAGTGCAAACCGTGGGGCTCATGATCGGCGGGGCGCAGGGCACTACCACGGCGGGAATCC‑GGGGTTTTGCCGCCCGTGTGTTtgcgggggttcgcgttgttttcgcgccc                                                                                                            >  1:691478/1‑228 (MQ=255)                                                                                          aTTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGGGCGCAGGGCCTTACCCCGGCGGTCATGC‑GGGTTTTTGCCGCCCGGGGGGTGGCGGGCGGGGt‑gatttgtttccggggcgc                                                                                                         >  1:3181/1‑236 (MQ=255)                                                                                           ttATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCggtt                                                                                                                                                                                                                           >  1:812750/1‑134 (MQ=255)                                                                                             atatGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGGGCGCAGGGCATTACCa                                                                                                                                                                           >  1:122704/1‑183 (MQ=255)                                                                                                        gTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCAcggcg                                                                                                                                                                      >  1:44188/1‑177 (MQ=255)                                                                                                              gCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGa                                                                                                                                                                                                   >  1:185577/1‑142 (MQ=255)                                                                                                               cGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATAAGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGc                                                                                                                                                                                  >  1:405546/1‑158 (MQ=255)                                                                                                                gTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTAc                                                                                                                                                                             <  1:670824/162‑1 (MQ=255)                                                                                                                 ttGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTACTGATTTTGTAAAA‑‑AGGGAAGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTAc                                                                              <  1:746923/251‑1 (MQ=255)                                                                                                                     gAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGTTTTTGAAAAA‑‑GGAAACGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCaa                                                                           <  1:805640/250‑1 (MQ=255)                                                                                                                          gTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGa                                                                                                                                                                                                   <  1:474059/130‑1 (MQ=255)                                                                                                                              ttGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGc                                                                                                                             <  1:726222/195‑1 (MQ=255)                                                                                                                                 cACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGa                                                              <  1:282353/250‑1 (MQ=255)                                                                                                                                 cACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGa                                                              <  1:482206/250‑1 (MQ=255)                                                                                                                                 cACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGa                                                              <  1:334875/250‑1 (MQ=255)                                                                                                                                 cACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGa                                                              <  1:276536/250‑1 (MQ=255)                                                                                                                                    ttGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑cc                                                         <  1:507204/251‑1 (MQ=255)                                                                                                                                                  ctcttGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTttgttg                                             <  1:539886/245‑1 (MQ=255)                                                                                                                                                             gAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGa                                                              <  1:109904/222‑1 (MQ=255)                                                                                                                                                                              ggCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGc                                                                                                                                                                                                                     <  1:714218/62‑1 (MQ=255)                                                                                                                                                                              ggCATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGGGCGCAGGGCATTACCACGGCGGGCATGC‑GGGTTTTAGCCGCCCGGGTGTTTGCGGGCGGGCT‑GGTTGATTTCCGGGGCC‑CCCACA‑CGATTGC‑GGGAACCCTTACCCAT‑AAGTTTGGGGGAG‑CCCGTGCTTTGTTGGGATTA‑GCCCT‑TGCGACGGTGA‑ttttta              >  1:898287/1‑249 (MQ=255)                                                                                                                                                                                cATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAAc             <  1:619568/250‑1 (MQ=255)                                                                                                                                                                                cATACGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAAc             <  1:176746/250‑1 (MQ=255)                                                                                                                                                                                   aCGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAAc             <  1:113706/247‑1 (MQ=255)                                                                                                                                                                                    cGGTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCAAGC‑GGGTTTTTGCCGCCGGTGTGTTGGCGGGGGTGCT‑GTTTTA‑TTCCGGTGCCCCCAAAACCAATTTC‑GAAAACAATT‑CCTAA‑AACTTGGGGGAAGCCCCGGGC‑TTGTTGGCATTA‑GTCTTCAG‑GACGAAGA‑TTTTAACAgttg        >  1:230556/1‑248 (MQ=255)                                                                                                                                                                                      gTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTTGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGGCGCCCGGGTGTTGGCGGGCGTGCT‑GATTGT‑TTTCCGTGCCGCCCACA‑CGATTGCGGGAAACC‑TTACCCATAAAGTGGGGGGAA‑‑CCCCCGCCTTGTTTGCATTATGCCTT‑‑GCGGCGATGA‑TTTTTAAAGCTGtt      >  1:574478/1‑251 (MQ=255)                                                                                                                                                                                      gTATGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGGTGGCGGGCGGGCT‑GATTGG‑TTTCGGGGCCGCCCACA‑CCATTGC‑GGGAACCATTTCCAAT‑AAAGTGGGGGGAG‑CCCGTGCTTTGTTGGCATTA‑GCCCT‑TTCGACGATGAGTTTTaaagccgtt       >  1:857085/1‑244 (MQ=255)                                                                                                                                                                                        aTGCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGGCATGC‑GGGTTTTAGCCGCCGGGGTGGTGGCGGGCGGGGTGGATTG‑‑TTTCGGGGGCGCCAACA‑AGGTTGC‑GGGAACCAATTCCCAT‑AAGTTGGGGGGAG‑CCCGGGCCTTGGTGGGATTA‑GCCTT‑TGCGACGAAGG‑TTTTTACAggagttgg    >  1:91509/1‑244 (MQ=255)                                                                                                                                                                                          gCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGAtt                                                                                                                                                                                                                           <  1:552882/44‑1 (MQ=255)                                                                                                                                                                                          gCTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAAc             <  1:34620/240‑1 (MQ=255)                                                                                                                                                                                           cTGGTGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAACAGCTGTTGGCg  <  1:869328/250‑1 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                                                                AAGATTTTTGTATTTTTATATAAAGCAATTTTTGCATTAAGGGCGAATAATCTCCTTTGTGTTATTTACACGGTTCATCACAGAGGAGAATTATATGACGACAGTCTCAGCGTTGGGAGCGGTCATTGCACTTGCGGTGGCGATCCTCCTGATTTTGAAAAA‑‑AGTATCGCCGGCATACGGTATGCTGGCGGGGGCGTTAATCGGCGGTTTGGTCGGCGGTGCCGATTTAGTGCAAACCGTGAGTCTCATGATCGGCGGTGCGCAGGGCATTACCACGGCGGTCATGC‑GGATTTTAGCCGCCGGTGTGTTGGCGGGCGTGCT‑GATTGA‑TTCCGGTGCCGCCAACA‑CGATTGC‑GGAAACCATTACCAAT‑AAGTTGGGGGAAG‑CCCGTGCTTTGTTGGCATTA‑GCCTT‑AGCGACGATGA‑TTTTAACAGCTGTTGGCG  >  1/143140‑143573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑