Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,875	C→A	S325S (TCG→TCT)	unknown ←	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,875	0	C	A	100.0%	166.0 / NA	62	S325S (TCG→TCT)	unknown	Inner membrane protein of type IV secretion of T‑DNA complex, TonB‑like, VirB10
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/31);  total (31/31)

AATCGCAATTTTTGTAATGGCTTCGTCTTCGAGCAATTCTCCGAATAATTTATTGGCATGAAGTAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCG  >  1/1136629‑1137121                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aaTCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGc                                                                                                                                                                                                                                                    <  1:103241/251‑1 (MQ=255)      cAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCgg                                                                                                                                                                                                                                                 >  1:24497/1‑249 (MQ=255)         tttctGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGt                                                                                                                                                                                                                                            >  1:804701/5‑251 (MQ=255)                aaTGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTAt                                                                                                                                                                                                                                     <  1:361486/251‑1 (MQ=255)                     cTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCTCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATcagtc                                                                                                                                                                                                                                <  1:824023/251‑5 (MQ=255)                     cTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATcagtc                                                                                                                                                                                                                                <  1:350983/251‑5 (MQ=255)                     cTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATcagtc                                                                                                                                                                                                                                <  1:491534/251‑5 (MQ=255)                                aGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATcagtcggattaaatg                                                                                                                                                                                                                      <  1:138197/250‑15 (MQ=255)                                aGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATcagtcggattaaatg                                                                                                                                                                                                                      <  1:69446/250‑15 (MQ=255)                                                             gagtgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTAc                                                                                                                                                                                                                                          <  1:139382/196‑1 (MQ=255)                                                             gagtgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATaatg                                                                                                                                                                                        >  1:83435/6‑248 (MQ=255)                                                                tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                     <  1:379915/249‑1 (MQ=255)                                                                tgaaaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                     <  1:271678/249‑1 (MQ=255)                                                                   aaTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTgg                                                                                                                                                                                  >  1:849504/1‑251 (MQ=255)                                                                    aTTGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGt                                                                                                                                                                                 >  1:367161/1‑251 (MQ=255)                                                                     ttGATACATCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGtt                                                                                                                                                                                >  1:393779/1‑251 (MQ=255)                                                                             tCGCCGGAAGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGt                                                                                                                                                                        >  1:711969/1‑251 (MQ=255)                                                                                    aaGAGTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATg                                                                                                                                                                 <  1:654303/251‑1 (MQ=255)                                                                                       agTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCt                                                                                                                                                               <  1:221211/250‑1 (MQ=255)                                                                                        gTTTATGGCCATGGTTTATACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCAc                                                                                                                                                                                     >  1:201633/1‑227 (MQ=255)                                                                                          ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                           <  1:468242/239‑1 (MQ=255)                                                                                          ttatggccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAAc                                                                                                                                                           <  1:103920/239‑1 (MQ=255)                                                                                                ccatggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                      <  1:505424/244‑1 (MQ=255)                                                                                                    ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                 >  1:212741‑M1/3‑251 (MQ=255)                                                                                                    ggtttatacCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAT                                                                                                                                                 >  1:514503‑M1/3‑251 (MQ=255)                                                                                                    ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCagag                                                                                                                                                                                                                                                     <  1:123032/149‑1 (MQ=255)                                                                                                    ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACat                                                                                                                                                         >  1:196232/3‑243 (MQ=255)                                                                                                    ggtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAATCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAt                                                                                                                                                 >  1:456171/3‑251 (MQ=255)                                                                                                     gtttatACCTCCTATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGc                                                                                                                                                                >  1:4890/2‑235 (MQ=255)                                                                                                              tcctATTTCTGTTTCAATTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagt                                                                                                                                       >  1:248387‑M1/1‑242 (MQ=255)                                                                                                                              aTTCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATaatg                                                                                                                                                                                        >  1:509628/1‑183 (MQ=255)                                                                                                                               ttCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgacc                                                                                                                        >  1:605604‑M1/1‑225 (MQ=255)                                                                                                                                tCGTATACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccggg                                                                                                                     >  1:288050‑M1/1‑224 (MQ=255)                                                                                                                                    atacaTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGAt                                                                                                                                                                                            >  1:153891/3‑176 (MQ=255)                                                                                                                                       caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                              <  1:482138‑M1/251‑35 (MQ=255)                                                                                                                                       caTCGCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttat                                                                                                              <  1:508186‑M1/251‑35 (MQ=255)                                                                                                                                           gCCAAAATCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatggg                                                                                                           >  1:291545‑M1/1‑213 (MQ=255)                                                                                                                                                aaTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggcttta                                                                                                      >  1:55230‑M1/1‑208 (MQ=255)                                                                                                                                                 aTCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacc                                                                                                    <  1:558750‑M1/251‑45 (MQ=255)                                                                                                                                                  tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACatgat                                                                                                                                                      <  1:157540/200‑1 (MQ=255)                                                                                                                                                  tCAATATCCCGCGCAACAAAAATCCCTACTAAATCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttac                                                                                                     >  1:877090‑M1/1‑206 (MQ=255)                                                                                                                                                                                 aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                    <  1:534921‑M1/251‑77 (MQ=255)                                                                                                                                                                                 aaTCCCCCTGGTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaata                                                                    <  1:657763‑M1/251‑77 (MQ=255)                                                                                                                                                                                          ggTTCTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgcctt                                                           >  1:465062‑M1/1‑166 (MQ=255)                                                                                                                                                                                              cTTATAAAGCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgc                                                       <  1:397013‑M1/251‑90 (MQ=255)                                                                                                                                                                                                      gCGTAGGCGGAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCAttgccaacagt                                                                                                                                       <  1:374985/163‑10 (MQ=255)                                                                                                                                                                                                               gAATATTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggt                                      >  1:556672‑M1/1‑145 (MQ=255)                                                                                                                                                                                                                  tatTAATGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaat                                                                     >  1:895880‑M1/1‑142 (MQ=255)                                                                                                                                                                                                                      aaTGGTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttac                               <  1:11000‑M1/251‑114 (MQ=255)                                                                                                                                                                                                                         ggTTTTCTCCAGGATACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgt                            >  1:723875‑M1/1‑135 (MQ=255)                                                                                                                                                                                                                              tctccaggatactttGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcag                       <  1:100574‑M1/244‑122 (MQ=255)                                                                                                                                                                                                                               ctccaggatacttTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagt                      >  1:649342‑M1/7‑129 (MQ=255)                                                                                                                                                                                                                                 ccaggatacTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttg                    >  1:445681‑M1/5‑127 (MQ=255)                                                                                                                                                                                                                                    ggaTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                 >  1:702748‑M1/2‑124 (MQ=255)                                                                                                                                                                                                                                      aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatct               <  1:389129‑M1/251‑130 (MQ=255)                                                                                                                                                                                                                                      aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                <  1:344739‑M1/250‑129 (MQ=255)                                                                                                                                                                                                                                      aTACTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatc                <  1:119187‑M1/250‑129 (MQ=255)                                                                                                                                                                                                                                        aCTTTGTGCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtat                 >  1:312015‑M1/1‑120 (MQ=255)                                                                                                                                                                                                                                               gCAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga      >  1:659247‑M1/1‑113 (MQ=255)                                                                                                                                                                                                                                                cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa     <  1:707011‑M1/251‑140 (MQ=255)                                                                                                                                                                                                                                                cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaa     <  1:257774‑M1/251‑140 (MQ=255)                                                                                                                                                                                                                                                cAATTTCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctga      <  1:223166‑M1/250‑139 (MQ=255)                                                                                                                                                                                                                                                   tttCAGAGCCGGCTTGTACCGTATCAGTCGGATTAAATGTCCCCCGTTTGGCTAATTGAGTAGATAATGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTgccaacagtaaattggcattgaccgggagtttatgggctttacctgctttaaacgtactgacgttatcttcaaatatatcgccttttgccggttccggtggttggttagttaccgtgtcagttgtatctgtattctgaacat  >  1:861862‑M1/1‑109 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AATCGCAATTTTTGTAATGGCTTCGTCTTCGAGCAATTCTCCGAATAATTTATTGGCATGAAGTAAAATTGATACATCGCCGGAAGAGTTTATGACCATTATTTATACCTCCTATTTCTGTTTCAATTCGTACACATCGCCAAAATCAATATCCCGCGCAACAAAAATCCCCACTAAATCCCCTTGGTTCTTATAAAGCGTAGGGGGAATATTAATGGTTTTCTCTAAGATACTTTGTGCAATTTCCGAGCCGGCTTGTACCGTATCCGTTGGTTGGAAAGTTCCGCGTTTTGCTAATTGGGTAGATAGTGCACTGGTTGAATCGGTGATCATGCTTAACATGATGGCATTACCGAAACGTTCCCAGAAATGATTATCTACATAGCCCGGAATACCGGTTCCGCCTAATTCATCGGTTGCGCCGGAATTAATGTTGATGATGATATTTTTCGGTGTACGGATTTCACTCCAGACTACAAATAAACGTTCTTCG  >  1/1136629‑1137121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑