Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,465	T→G	N88T (AAC→ACC)	unknown ←	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,465	0	T	G	100.0%	167.7 / NA	64	N88T (AAC→ACC)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/34);  total (30/34)

TGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAAAATTGGTTTGGTATCACTGATATAATCCCCGCGATACTTGGCTAACGCCCGAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACGCCTTTTTTTTCAAAGAAAATTTCACCGGGGCGATTAATCGCAATTTTTGTAATGGCTTCGTCTTCGAGCAATTCTCCGAATAATTTATTGGCATGAAGTAAAATTGATAC  >  1/1136249‑1136703                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               tGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGGAATACCGCAGATAAAATCGGTTTAGTAt                                                                                                                                                                                                               >  1:619725/1‑250 (MQ=255)  gTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCa                                                                                                                                                                                                             >  1:186552/1‑251 (MQ=255)   tttAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCAc                                                                                                                                                                                                            <  1:635848/251‑1 (MQ=255)          aaTTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCAGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGatat                                                                                                                                                                                                      <  1:343047/250‑1 (MQ=255)           aTTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATaa                                                                                                                                                                                                    <  1:648888/251‑1 (MQ=255)            ttCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAAAAATCTGTGTCCGCTCGCCCGTCGGGAATACCGCAGATAAAAACGGTTTAGTATCACTTATATAAt                                                                                                                                                                                                   >  1:240064/1‑251 (MQ=255)             tCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATc                                                                                                                                                                                                  <  1:841119/251‑1 (MQ=255)                  aTAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATc                                                                                                                                                                                                  <  1:353341/246‑1 (MQ=255)                     atcacccTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATc                                                                                                                                                                                                  <  1:263312/239‑1 (MQ=255)                         cccTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGGCGGTAATACCGCAGATAAAAAAGGGTTAGTATCACTGATATAATCCCCACCATACtt                                                                                                                                                                                      >  1:109856/1‑251 (MQ=255)                                   aaaTTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGcc                                                                                                                                                                            <  1:893816/251‑1 (MQ=255)                                       ttCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTg                                                                                                                                                                                     >  1:852425/1‑238 (MQ=255)                                       ttCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCCGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACCTGGCTAACGCCCgt                                                                                                                                                                         >  1:575718/1‑249 (MQ=255)                                       ttCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCCCGCCGGGCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGatat                                                                                                                                                                                                      >  1:692709/1‑221 (MQ=255)                                       ttCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAAAAATCTTTGTCCGCTCGCCGGTCGGGAATAc                                                                                                                                                                                                                                      >  1:75492/1‑189 (MQ=255)                                            gtagtGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAAAAATCTGTGTCCGCTCGCCGGGCGGTAATACCGCCGATAAAATCGGTTTAGTATCACTGATATAATCCCCCCGATACTTGGCTAACGCCCTAGCaaaa                                                                                                                                                                   >  1:476477/1‑251 (MQ=255)                                            gtagtGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTg                                                                                                                                                                                                          <  1:723338/212‑1 (MQ=255)                                            gtagtGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGatat                                                                                                                                                                                                      >  1:886079/1‑216 (MQ=255)                                               gtgtCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAAt                                                                                                                                                                                                                                        <  1:316800/179‑1 (MQ=255)                                                      gTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAAATTTTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAAACGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCt                                                                                                                                                                                  >  1:136030/1‑226 (MQ=255)                                                                gACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGa                                                                                                                                                                 <  1:26571/233‑1 (MQ=255)                                                                         ttttCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTAGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCgg                                                                                                                                      <  1:260284/251‑1 (MQ=255)                                                                          tttCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGGTTAGTATCACTGATATAATCCCCACGATACTTGGc                                                                                                                                                                                   >  1:866800/1‑205 (MQ=255)                                                                               gTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATc                                                                                                                                                     <  1:807235/230‑1 (MQ=255)                                                                               gTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATaact                                                                                                                                               <  1:587945/236‑3 (MQ=255)                                                                                     aTAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGAt                                                                                                                           <  1:601011/250‑1 (MQ=255)                                                                                     aTAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATa                                                                                                                          <  1:91388/251‑1 (MQ=255)                                                                                     aTAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATa                                                                                                                          <  1:840678/251‑1 (MQ=255)                                                                                                     aGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCCGGAGAATAATATCTGTCCGCTCGCCGGTCGGCAATACCGCCGATAAAATCGGATTAGTATCACTGATATAATCCCCACGATACTTTGCTAACCCCCTAGCAAAAGAATCACACAAATCATAAGAAATATTCGCAGAGTCTTGATAATGCCATAcacccttt                                                                                                          >  1:862818/1‑244 (MQ=255)                                                                                                       ccGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCtttt                                                                                                          <  1:549671/249‑1 (MQ=255)                                                                                                       ccGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCtttt                                                                                                          <  1:703281/249‑1 (MQ=255)                                                                                                        cGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCtttttgt                                                                                                       <  1:410046/251‑3 (MQ=255)                                                                                                                  gTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAg                                                                                                                                                                  <  1:316279/182‑1 (MQ=255)                                                                                                                  gTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGc                                                                                                                                        <  1:107738/208‑1 (MQ=255)                                                                                                                  gTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGGAATACCGCAGATAAAAACGGTTTTGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAAACataaataa                                                                                                                                             >  1:568366/1‑203 (MQ=255)                                                                                                                  gTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCCCGCCGGGCGGTAATACCGCAGATAAAATCGGGTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATaaa                                                                                                                                                >  1:624721/1‑199 (MQ=255)                                                                                                                   taataaTCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCGGGCGGGAATACCGCAGATAAAATCGGTTTTGTATCACTGatat                                                                                                                                                                                                      >  1:566053/1‑145 (MQ=255)                                                                                                                   taataaTCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATa                                                                                                                          <  1:822154/221‑1 (MQ=255)                                                                                                                   taataaTCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCtttt                                                                                                          <  1:164953/237‑1 (MQ=255)                                                                                                                    aataatCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAATAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTTAAAGAAAAtt                                                                                           >  1:667276/1‑251 (MQ=255)                                                                                                                              gTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCggg                                                                                   <  1:219327/249‑1 (MQ=255)                                                                                                                                  aaaaaaGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAAACGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAAAAACACATAAAACATAAATAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTTTTCAAAGAAAATTTTACCgggac                                                                                 >  1:824531/1‑245 (MQ=255)                                                                                                                                   aaaaaGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCCCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCCCGATACTTGGCTAACGCCCTAGCAAAAAAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCCTACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTa                                                                            >  1:148260/1‑251 (MQ=255)                                                                                                                                        gACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATAc                                                                                                                 <  1:192166/209‑1 (MQ=255)                                                                                                                                        gACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAAACGGTTTAGTATCACTGATATAATCCCCCCGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATa                                                                                                                          >  1:210551/1‑200 (MQ=255)                                                                                                                                                  gTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGGCGGTAATACCGCAGATAAAATAGGTTTAGGATCACCGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAAAATCACATAAAACATAAataata                                                                                                                                           >  1:773714/1‑173 (MQ=255)                                                                                                                                                         ccccGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAACCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGTCGATTAATCGCAATTTCTGTAATGGCt                                                       <  1:81625/248‑1 (MQ=255)                                                                                                                                                           ccGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCCCCGGGCGGGAATACCGCCGATAAAAACCGTTTTGTATCACTGATATAATTCCCACCATACTTGGCTAACGCCCTAGCAAAAGAAACACATAAATCATAAGTAATATGCGGAGCGTCTTTATAATGCCATACACCCTTTTGTTTAAAAAAAATTTCACCCGGGCGATTAATCGCACTTTCTGTTATTGCTTCg                                                    >  1:313833/1‑251 (MQ=255)                                                                                                                                                           ccGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTTGTATCACTGATATAATCCCCACGATACTTGGCTAAAGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGGGCGTCTTGATAATGCCATACACCTTTTTGTTTAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCg                                                    >  1:516806/1‑251 (MQ=255)                                                                                                                                                             gTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATaa                                                                                                                                                        <  1:849132/149‑1 (MQ=255)                                                                                                                                                             gTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGc                                                                                                                      <  1:855724/183‑1 (MQ=255)                                                                                                                                                             gTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCCCCCCCGTCGGGAATACCGCAGATAAAAACGGTTTAGTATCACTGATATAATCCCCCCGATACTTTGCTAACGCCCTAGCAAAAAAATCACATAAATCATAAGTAATATGCGGAGc                                                                                                                                   >  1:371532/1‑170 (MQ=255)                                                                                                                                                                  aCTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCCGTCGGGAATACCGCAGATAAAATCGGGTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCgg                                             >  1:183477/1‑250 (MQ=255)                                                                                                                                                                  aCTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCCCTCGCCGGTCGGTAATACCGCAGATAAAAACGGTTTTGTATCACTGATATAAACCCCACGATACTTGGCTAACGCCCTAGCAAAAGAAACACATAAATCATAAATAATATGCGGAGCGTCtt                                                                                                                              >  1:219757/1‑170 (MQ=255)                                                                                                                                                                                  tCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATgg                                                         >  1:242152/1‑223 (MQ=255)                                                                                                                                                                                                  gaaTAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTaa                                                                                                                                             <  1:168994/123‑1 (MQ=255)                                                                                                                                                                                                  gaaTAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGc                                           <  1:563325/221‑1 (MQ=255)                                                                                                                                                                                                       atCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAAt        <  1:49024/251‑1 (MQ=255)                                                                                                                                                                                                       atCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAAt        >  1:324075/1‑251 (MQ=255)                                                                                                                                                                                                       atCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAAt        >  1:351912/1‑251 (MQ=255)                                                                                                                                                                                                         cTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTg      <  1:422789/251‑1 (MQ=255)                                                                                                                                                                                                           gtgtCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGAt    >  1:675703/1‑251 (MQ=255)                                                                                                                                                                                                              tCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATAc  <  1:236705/250‑1 (MQ=255)                                                                                                                                                                                                              tCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCACTGATATAATCCCCACGATACTTGGCTAACGCCCTAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACACCTTTTTGTTCAAAGAAAATTTCACCGGGACGATTAATCGCAATTTCTGTAATGGCTTCGCCTTCGAGCAATTTTCCGAATAATTTATTGGCATGGAGTGAAATTGATAc  <  1:884028/250‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAAAATTGGTTTGGTATCACTGATATAATCCCCGCGATACTTGGCTAACGCCCGAGCAAAAGAATCACATAAATCATAAGTAATATGCGGAGCGTCTTGATAATGCCATACGCCTTTTTTTTCAAAGAAAATTTCACCGGGGCGATTAATCGCAATTTTTGTAATGGCTTCGTCTTCGAGCAATTCTCCGAATAATTTATTGGCATGAAGTAAAATTGATAC  >  1/1136249‑1136703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑