Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,257	A→G	F157F (TTT→TTC)	unknown ←	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,257	0	A	G	100.0%	202.8 / NA	70	F157F (TTT→TTC)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/37);  total (33/37)

CAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAAAATTGGTTTGGTATCAC  >  1/1136021‑1136501                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     cAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAAt                                                                                                                                                                                                                                         <  1:420622/250‑1 (MQ=255)       ttCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGTGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCtt                                                                                                                                                                                                                                  <  1:437037/251‑1 (MQ=255)            cAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                                                              <  1:806088/250‑1 (MQ=255)                      gCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                                                              <  1:437769/240‑1 (MQ=255)                          tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                                                <  1:161532/250‑1 (MQ=255)                          tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                                                <  1:310402/250‑1 (MQ=255)                          tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                                                <  1:467232/250‑1 (MQ=255)                                            ttGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGAc                                                                                                                                                                                             <  1:834854/251‑1 (MQ=255)                                              gCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATGAATTTCCAGTAGTGTCCAGTCATCATCTGACaa                                                                                                                                                                                           >  1:620303/1‑251 (MQ=255)                                               cTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACaaa                                                                                                                                                                                          <  1:63083/251‑1 (MQ=255)                                                  cGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTcc                                                                                                                                                                                                                     >  1:282654/1‑221 (MQ=255)                                                        gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                                                >  1:598776/1‑220 (MQ=255)                                                        gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCa                                                                                                                                                                                                                    <  1:392256/216‑1 (MQ=255)                                                         aTAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCg                                                                                                                                                                                <  1:700861/251‑1 (MQ=255)                                                            gAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGt                                                                                                                                                                               >  1:121596/1‑249 (MQ=255)                                                                 aGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATa                                                                                                                                                                        >  1:82622/1‑251 (MQ=255)                                                                  gTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGc                                                                                                                                                                                                                                              <  1:479340/180‑1 (MQ=255)                                                                           gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                                                 <  1:843645/215‑1 (MQ=255)                                                                           gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaa                                                                                                                                                              >  1:697758/2‑251 (MQ=255)                                                                              cTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                                                             >  1:566925/1‑185 (MQ=255)                                                                                 aTGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTc                                                                                                                                                                              <  1:92988/229‑1 (MQ=255)                                                                                        cGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCgttgtt                                                                                                                                                 >  1:697527/1‑251 (MQ=255)                                                                                              aataatTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGta                                                                                                                                           <  1:880128/251‑1 (MQ=255)                                                                                                     ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    <  1:72941/251‑1 (MQ=255)                                                                                                     ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    <  1:333253/251‑1 (MQ=255)                                                                                                     ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    <  1:667406/251‑1 (MQ=255)                                                                                                       ggggTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    <  1:222634/246‑1 (MQ=255)                                                                                                                 caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                                                 >  1:796907/3‑178 (MQ=255)                                                                                                                 caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCaaaaaaa                                                                                                                        >  1:118863/3‑251 (MQ=255)                                                                                                                    tggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGAc                                                                                                                     <  1:564395/243‑1 (MQ=255)                                                                                                                     ggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    <  1:569426/228‑1 (MQ=255)                                                                                                                      gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                                                             >  1:567171/7‑145 (MQ=255)                                                                                                                      gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACa                                                                                                                                                                                            <  1:241233/172‑1 (MQ=255)                                                                                                                      gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaaaa                                                                                                                                                            >  1:713014/7‑210 (MQ=255)                                                                                                                      gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                                                   >  1:193704/7‑251 (MQ=255)                                                                                                                      gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                                                   >  1:728520/7‑251 (MQ=255)                                                                                                                          ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCATAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                                                               <  1:423158/249‑1 (MQ=255)                                                                                                                          ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                                                               <  1:37453/249‑1 (MQ=255)                                                                                                                               cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACACACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGtaataa                                                                                                                                       >  1:441469/1‑222 (MQ=255)                                                                                                                               cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGttt                                                                                                          >  1:213533/1‑251 (MQ=255)                                                                                                                                       cAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCg                                                                                                  >  1:508718/1‑251 (MQ=255)                                                                                                                                            gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    >  1:381927/1‑212 (MQ=255)                                                                                                                                            gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                                                             >  1:148275/1‑251 (MQ=255)                                                                                                                                             aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                                            <  1:235783/251‑1 (MQ=255)                                                                                                                                             aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                                            <  1:720757/251‑1 (MQ=255)                                                                                                                                              atatAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATc                                                                                                                                     <  1:5820/209‑1 (MQ=255)                                                                                                                                               tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                                                             >  1:472662/1‑248 (MQ=255)                                                                                                                                               tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                                                          <  1:665791/251‑1 (MQ=255)                                                                                                                                                   aTCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATgg                                                                                      <  1:529214/251‑1 (MQ=255)                                                                                                                                                     cGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGtca                                                                                                                                                                                                      >  1:560036/1‑137 (MQ=255)                                                                                                                                                      gactaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTcc                                                                                                                                                                                 >  1:876584/5‑157 (MQ=255)                                                                                                                                                       actaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                                                          <  1:157689/240‑1 (MQ=255)                                                                                                                                                                  cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                                    >  1:722982/1‑190 (MQ=255)                                                                                                                                                                  cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                                                       >  1:228276/1‑251 (MQ=255)                                                                                                                                                                  cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                                                       <  1:234183/251‑1 (MQ=255)                                                                                                                                                                            gTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGgcgggcgggag                                                             >  1:603971/1‑248 (MQ=255)                                                                                                                                                                                tCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaat                                                          <  1:677345/250‑1 (MQ=255)                                                                                                                                                                                 cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                                                        <  1:611625/251‑1 (MQ=255)                                                                                                                                                                                 cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                                                        <  1:473939/251‑1 (MQ=255)                                                                                                                                                                                                      aaTCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCgggcg                                   >  1:69356/1‑247 (MQ=255)                                                                                                                                                                                                             atatTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                                            >  1:239679/1‑187 (MQ=255)                                                                                                                                                                                                                      ccGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAAAAATCTGTGTCcgcccgcccggcggtaaaaccgccgaaaa                   >  1:184635/1‑230 (MQ=255)                                                                                                                                                                                                                                     tGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                                            >  1:879769/1‑163 (MQ=255)                                                                                                                                                                                                                                     tGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGGAATACCGCAGATAAAATCGGTTTAGTAt     >  1:619725/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAg                                                                                                                                                         >  1:311041/1‑101 (MQ=255)                                                                                                                                                                                                                                      gTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                                            >  1:711394/1‑162 (MQ=255)                                                                                                                                                                                                                                      gTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCa   >  1:186552/1‑251 (MQ=255)                                                                                                                                                                                                                                       tttAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGGTCGGTAATACCGCAGATAAAATCGGTTTAGTATCAc  <  1:635848/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                                                        <  1:839962/196‑1 (MQ=255)                                                                                                                                                                                                                                        ttAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATc                                                      <  1:341212/198‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     CAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAAAATTGGTTTGGTATCAC  >  1/1136021‑1136501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑