Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,136,239 G→A I163I (ATC→ATT)  unknown ← ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,136,2390GA100.0% 193.0 / NA 68I163I (ATC→ATT) unknownATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/38);  total (30/38)

GGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAA  >  1/1136006‑1136484
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
ggTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGATTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAg                                                                                                                                                                                                                                      >  1:682227/1‑251 (MQ=255)
  tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCTCAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                                                                                     <  1:614619/250‑2 (MQ=255)
  tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                                    <  1:830234/251‑3 (MQ=255)
  tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                                    <  1:637024/251‑3 (MQ=255)
  tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                                                                                     <  1:153121/250‑2 (MQ=255)
               cAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAAt                                                                                                                                                                                                                        <  1:420622/250‑1 (MQ=255)
                     ttCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGTGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCtt                                                                                                                                                                                                                 <  1:437037/251‑1 (MQ=255)
                          cAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                                             <  1:806088/250‑1 (MQ=255)
                                    gCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                                             <  1:437769/240‑1 (MQ=255)
                                        tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                               <  1:161532/250‑1 (MQ=255)
                                        tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                               <  1:467232/250‑1 (MQ=255)
                                        tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                               <  1:310402/250‑1 (MQ=255)
                                                          ttGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGAc                                                                                                                                                                            <  1:834854/251‑1 (MQ=255)
                                                            gCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATGAATTTCCAGTAGTGTCCAGTCATCATCTGACaa                                                                                                                                                                          >  1:620303/1‑251 (MQ=255)
                                                             cTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACaaa                                                                                                                                                                         <  1:63083/251‑1 (MQ=255)
                                                                cGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTcc                                                                                                                                                                                                    >  1:282654/1‑221 (MQ=255)
                                                                      gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                                               >  1:598776/1‑220 (MQ=255)
                                                                      gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCa                                                                                                                                                                                                   <  1:392256/216‑1 (MQ=255)
                                                                       aTAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCg                                                                                                                                                               <  1:700861/251‑1 (MQ=255)
                                                                          gAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGt                                                                                                                                                              >  1:121596/1‑249 (MQ=255)
                                                                               aGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATa                                                                                                                                                       >  1:82622/1‑251 (MQ=255)
                                                                                gTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGc                                                                                                                                                                                                                             <  1:479340/180‑1 (MQ=255)
                                                                                         gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                                <  1:843645/215‑1 (MQ=255)
                                                                                         gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaa                                                                                                                                             >  1:697758/2‑251 (MQ=255)
                                                                                            cTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                                            >  1:566925/1‑185 (MQ=255)
                                                                                               aTGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTc                                                                                                                                                             <  1:92988/229‑1 (MQ=255)
                                                                                                      cGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCgttgtt                                                                                                                                >  1:697527/1‑251 (MQ=255)
                                                                                                            aataatTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGta                                                                                                                          <  1:880128/251‑1 (MQ=255)
                                                                                                                   ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   <  1:72941/251‑1 (MQ=255)
                                                                                                                   ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   <  1:667406/251‑1 (MQ=255)
                                                                                                                   ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   <  1:333253/251‑1 (MQ=255)
                                                                                                                     ggggTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   <  1:222634/246‑1 (MQ=255)
                                                                                                                               caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                                >  1:796907/3‑178 (MQ=255)
                                                                                                                               caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCaaaaaaa                                                                                                       >  1:118863/3‑251 (MQ=255)
                                                                                                                                  tggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGAc                                                                                                    <  1:564395/243‑1 (MQ=255)
                                                                                                                                   ggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   <  1:569426/228‑1 (MQ=255)
                                                                                                                                    gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                                            >  1:567171/7‑145 (MQ=255)
                                                                                                                                    gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACa                                                                                                                                                                           <  1:241233/172‑1 (MQ=255)
                                                                                                                                    gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaaaa                                                                                                                                           >  1:713014/7‑210 (MQ=255)
                                                                                                                                    gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                                  >  1:193704/7‑251 (MQ=255)
                                                                                                                                    gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                                  >  1:728520/7‑251 (MQ=255)
                                                                                                                                        ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCATAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                                              <  1:423158/249‑1 (MQ=255)
                                                                                                                                        ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                                              <  1:37453/249‑1 (MQ=255)
                                                                                                                                             cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACACACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGtaataa                                                                                                                      >  1:441469/1‑222 (MQ=255)
                                                                                                                                             cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGttt                                                                                         >  1:213533/1‑251 (MQ=255)
                                                                                                                                                     cAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCg                                                                                 >  1:508718/1‑251 (MQ=255)
                                                                                                                                                       aCGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                                    >  1:253923/1‑100 (MQ=255)
                                                                                                                                                          gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   >  1:381927/1‑212 (MQ=255)
                                                                                                                                                          gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                                            >  1:148275/1‑251 (MQ=255)
                                                                                                                                                           aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                           <  1:235783/251‑1 (MQ=255)
                                                                                                                                                           aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                           <  1:720757/251‑1 (MQ=255)
                                                                                                                                                            atatAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATc                                                                                                                    <  1:5820/209‑1 (MQ=255)
                                                                                                                                                             tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                                            >  1:472662/1‑248 (MQ=255)
                                                                                                                                                             tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                                         <  1:665791/251‑1 (MQ=255)
                                                                                                                                                                 aTCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATgg                                                                     <  1:529214/251‑1 (MQ=255)
                                                                                                                                                                   cGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGtca                                                                                                                                                                                     >  1:560036/1‑137 (MQ=255)
                                                                                                                                                                    gactaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTcc                                                                                                                                                                >  1:876584/5‑157 (MQ=255)
                                                                                                                                                                     actaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                                         <  1:157689/240‑1 (MQ=255)
                                                                                                                                                                                cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                                   >  1:722982/1‑190 (MQ=255)
                                                                                                                                                                                cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                                      >  1:228276/1‑251 (MQ=255)
                                                                                                                                                                                cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                                      <  1:234183/251‑1 (MQ=255)
                                                                                                                                                                                          gTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGgcgggcgggag                                            >  1:603971/1‑248 (MQ=255)
                                                                                                                                                                                              tCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaat                                         <  1:677345/250‑1 (MQ=255)
                                                                                                                                                                                               cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                                       <  1:611625/251‑1 (MQ=255)
                                                                                                                                                                                               cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                                       <  1:473939/251‑1 (MQ=255)
                                                                                                                                                                                                                    aaTCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCgggcg                  >  1:69356/1‑247 (MQ=255)
                                                                                                                                                                                                                           atatTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                                           >  1:239679/1‑187 (MQ=255)
                                                                                                                                                                                                                                    ccGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAAAAATCTGTGTCcgcccgcccggcggtaaaaccgccgaaaa  >  1:184635/1‑230 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
GGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCGGTAATACCGCAGATAA  >  1/1136006‑1136484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑