Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,136,230	C→T	K166K (AAG→AAA)	unknown ←	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,136,230	0	C	T	100.0%	187.2 / NA	69	K166K (AAG→AAA)	unknown	ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/38);  total (31/38)

GTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCG  >  1/1135992‑1136468                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCaaatg                                                                                                                                                                                                                                    >  1:809806/1‑247 (MQ=255) gTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCaaatg                                                                                                                                                                                                                                    >  1:714019/1‑247 (MQ=255)               ggTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGATTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAg                                                                                                                                                                                                                      >  1:682227/1‑251 (MQ=255)                 tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCTCAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                                                                     <  1:614619/250‑2 (MQ=255)                 tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                    <  1:830234/251‑3 (MQ=255)                 tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                    <  1:637024/251‑3 (MQ=255)                 tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                                                                     <  1:153121/250‑2 (MQ=255)                              cAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAAt                                                                                                                                                                                                        <  1:420622/250‑1 (MQ=255)                                    ttCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGTGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCtt                                                                                                                                                                                                 <  1:437037/251‑1 (MQ=255)                                         cAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                             <  1:806088/250‑1 (MQ=255)                                                   gCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                                                                             <  1:437769/240‑1 (MQ=255)                                                       tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                               <  1:467232/250‑1 (MQ=255)                                                       tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                               <  1:310402/250‑1 (MQ=255)                                                       tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                               <  1:161532/250‑1 (MQ=255)                                                                         ttGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGAc                                                                                                                                                            <  1:834854/251‑1 (MQ=255)                                                                           gCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATGAATTTCCAGTAGTGTCCAGTCATCATCTGACaa                                                                                                                                                          >  1:620303/1‑251 (MQ=255)                                                                            cTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACaaa                                                                                                                                                         <  1:63083/251‑1 (MQ=255)                                                                               cGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTcc                                                                                                                                                                                    >  1:282654/1‑221 (MQ=255)                                                                                     gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                                                                               >  1:598776/1‑220 (MQ=255)                                                                                     gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCa                                                                                                                                                                                   <  1:392256/216‑1 (MQ=255)                                                                                      aTAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCg                                                                                                                                               <  1:700861/251‑1 (MQ=255)                                                                                         gAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGt                                                                                                                                              >  1:121596/1‑249 (MQ=255)                                                                                              aGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATa                                                                                                                                       >  1:82622/1‑251 (MQ=255)                                                                                               gTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGc                                                                                                                                                                                                             <  1:479340/180‑1 (MQ=255)                                                                                                        gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                <  1:843645/215‑1 (MQ=255)                                                                                                        gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaa                                                                                                                             >  1:697758/2‑251 (MQ=255)                                                                                                           cTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                            >  1:566925/1‑185 (MQ=255)                                                                                                              aTGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTc                                                                                                                                             <  1:92988/229‑1 (MQ=255)                                                                                                                     cGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCgttgtt                                                                                                                >  1:697527/1‑251 (MQ=255)                                                                                                                           aataatTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGta                                                                                                          <  1:880128/251‑1 (MQ=255)                                                                                                                                  ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   <  1:72941/251‑1 (MQ=255)                                                                                                                                  ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   <  1:667406/251‑1 (MQ=255)                                                                                                                                  ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   <  1:333253/251‑1 (MQ=255)                                                                                                                                    ggggTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   <  1:222634/246‑1 (MQ=255)                                                                                                                                              caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                                                                                >  1:796907/3‑178 (MQ=255)                                                                                                                                              caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCaaaaaaa                                                                                       >  1:118863/3‑251 (MQ=255)                                                                                                                                                 tggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGAc                                                                                    <  1:564395/243‑1 (MQ=255)                                                                                                                                                  ggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   <  1:569426/228‑1 (MQ=255)                                                                                                                                                   gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                                                                            >  1:567171/7‑145 (MQ=255)                                                                                                                                                   gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACa                                                                                                                                                           <  1:241233/172‑1 (MQ=255)                                                                                                                                                   gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaaaa                                                                                                                           >  1:713014/7‑210 (MQ=255)                                                                                                                                                   gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                  >  1:728520/7‑251 (MQ=255)                                                                                                                                                   gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                                                                                  >  1:193704/7‑251 (MQ=255)                                                                                                                                                       ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCATAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                              <  1:423158/249‑1 (MQ=255)                                                                                                                                                       ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                                                                              <  1:37453/249‑1 (MQ=255)                                                                                                                                                            cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACACACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGtaataa                                                                                                      >  1:441469/1‑222 (MQ=255)                                                                                                                                                            cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGttt                                                                         >  1:213533/1‑251 (MQ=255)                                                                                                                                                                    cAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCg                                                                 >  1:508718/1‑251 (MQ=255)                                                                                                                                                                      aCGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                                                                    >  1:253923/1‑100 (MQ=255)                                                                                                                                                                         gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   >  1:381927/1‑212 (MQ=255)                                                                                                                                                                         gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                            >  1:148275/1‑251 (MQ=255)                                                                                                                                                                          aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                           <  1:235783/251‑1 (MQ=255)                                                                                                                                                                          aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                           <  1:720757/251‑1 (MQ=255)                                                                                                                                                                           atatAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATc                                                                                                    <  1:5820/209‑1 (MQ=255)                                                                                                                                                                            tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa                                                            >  1:472662/1‑248 (MQ=255)                                                                                                                                                                            tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                         <  1:665791/251‑1 (MQ=255)                                                                                                                                                                                aTCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATgg                                                     <  1:529214/251‑1 (MQ=255)                                                                                                                                                                                  cGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGtca                                                                                                                                                                     >  1:560036/1‑137 (MQ=255)                                                                                                                                                                                   gactaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTcc                                                                                                                                                >  1:876584/5‑157 (MQ=255)                                                                                                                                                                                    actaGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg                                                         <  1:157689/240‑1 (MQ=255)                                                                                                                                                                                               cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                                                                   >  1:722982/1‑190 (MQ=255)                                                                                                                                                                                               cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                      <  1:234183/251‑1 (MQ=255)                                                                                                                                                                                               cATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTgg                                      >  1:228276/1‑251 (MQ=255)                                                                                                                                                                                                         gTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGgcgggcgggag                            >  1:603971/1‑248 (MQ=255)                                                                                                                                                                                                             tCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaat                         <  1:677345/250‑1 (MQ=255)                                                                                                                                                                                                              cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                       <  1:611625/251‑1 (MQ=255)                                                                                                                                                                                                              cccGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGaataa                       <  1:473939/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGGAGAATAATCTGTGTCCGCTCGCCgggcg  >  1:69356/1‑247 (MQ=255)                                                                                                                                                                                                                                          atatTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc                                                           >  1:239679/1‑187 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGGTTTCCCGTTCACTGGCGGGCGGAAGAATAATCTGTGTCCGCTCGCCGTTCG  >  1/1135992‑1136468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑