Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,136,173 C→T L185L (CTG→CTA)  unknown ← ATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,136,1730CT100.0% 190.7 / NA 69L185L (CTG→CTA) unknownATPase required for both assembly of type IV secretion complex and secretion of T‑DNA complex, VirB11
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/41);  total (28/41)

CCGTTATGCCCGGAAGAAACCACATTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGG  >  1/1135928‑1136417
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
ccGTTATGCCCGGAAGAAACCACATTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGc                                                                                                                                                                                                                                                 <  1:520970/251‑1 (MQ=255)
       gCCCGGAAGAAACCACATTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCAtt                                                                                                                                                                                                                                          >  1:119083/1‑251 (MQ=255)
            gaagaaACCACATTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAg                                                                                                                                                                                                                                      <  1:263081/250‑1 (MQ=255)
                      caTTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCg                                                                                                                                                                                                                           >  1:323651/1‑251 (MQ=255)
                                gTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAggg                                                                                                                                                                                                                                                  <  1:104685/218‑1 (MQ=255)
                                 tCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTc                                                                                                                                                                                                                <  1:608713/251‑1 (MQ=255)
                                       gTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTccc                                                                                                                                                                                                                            <  1:325609/233‑1 (MQ=255)
                                                  tcgTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAatat                                                                                                                                                                                               <  1:255552/250‑1 (MQ=255)
                                                           cGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATcac                                                                                                                                                                                                    <  1:166111/237‑1 (MQ=255)
                                                                gTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCaaatg                                                                                                                                                                                 >  1:809806/1‑247 (MQ=255)
                                                                gTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCaaatg                                                                                                                                                                                 >  1:714019/1‑247 (MQ=255)
                                                                              ggTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGATTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAg                                                                                                                                                                   >  1:682227/1‑251 (MQ=255)
                                                                                tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCTCAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                  <  1:614619/250‑2 (MQ=255)
                                                                                tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                 <  1:830234/251‑3 (MQ=255)
                                                                                tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                 <  1:637024/251‑3 (MQ=255)
                                                                                tttCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgg                                                                                                                                                                  <  1:153121/250‑2 (MQ=255)
                                                                                             cAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAAt                                                                                                                                                     <  1:420622/250‑1 (MQ=255)
                                                                                                   ttCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGTGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCtt                                                                                                                                              <  1:437037/251‑1 (MQ=255)
                                                                                                        cAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                          <  1:806088/250‑1 (MQ=255)
                                                                                                                  gCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGa                                                                                                                                          <  1:437769/240‑1 (MQ=255)
                                                                                                                      tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                            <  1:467232/250‑1 (MQ=255)
                                                                                                                      tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                            <  1:310402/250‑1 (MQ=255)
                                                                                                                      tGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                            <  1:161532/250‑1 (MQ=255)
                                                                                                                                        ttGCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGAc                                                                                                         <  1:834854/251‑1 (MQ=255)
                                                                                                                                          gCTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATGAATTTCCAGTAGTGTCCAGTCATCATCTGACaa                                                                                                       >  1:620303/1‑251 (MQ=255)
                                                                                                                                           cTTCGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACaaa                                                                                                      <  1:63083/251‑1 (MQ=255)
                                                                                                                                              cGCTTGGATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTcc                                                                                                                                 >  1:282654/1‑221 (MQ=255)
                                                                                                                                                    gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCagtag                                                                                                                            >  1:598776/1‑220 (MQ=255)
                                                                                                                                                    gATAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCa                                                                                                                                <  1:392256/216‑1 (MQ=255)
                                                                                                                                                     aTAGAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCg                                                                                            <  1:700861/251‑1 (MQ=255)
                                                                                                                                                        gAATAAGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGt                                                                                           >  1:121596/1‑249 (MQ=255)
                                                                                                                                                             aGTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATa                                                                                    >  1:82622/1‑251 (MQ=255)
                                                                                                                                                              gTGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGc                                                                                                                                                          <  1:479340/180‑1 (MQ=255)
                                                                                                                                                               tGGAAATAGTTCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAggg                                                                                                                                                                                                                                                  >  1:187583/1‑91 (MQ=255)
                                                                                                                                                                       gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                             <  1:843645/215‑1 (MQ=255)
                                                                                                                                                                       gttCTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaa                                                                          >  1:697758/2‑251 (MQ=255)
                                                                                                                                                                          cTTATGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                         >  1:566925/1‑185 (MQ=255)
                                                                                                                                                                             aTGTTGGCGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTc                                                                                          <  1:92988/229‑1 (MQ=255)
                                                                                                                                                                                    cGTAAGAATAATTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCgttgtt                                                             >  1:697527/1‑251 (MQ=255)
                                                                                                                                                                                          aataatTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGta                                                       <  1:880128/251‑1 (MQ=255)
                                                                                                                                                                                              atTCCGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAg                                                                                                                                                                                                                                      <  1:323454/72‑1 (MQ=255)
                                                                                                                                                                                                 ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                <  1:72941/251‑1 (MQ=255)
                                                                                                                                                                                                 ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                <  1:333253/251‑1 (MQ=255)
                                                                                                                                                                                                 ccGGGGTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                <  1:667406/251‑1 (MQ=255)
                                                                                                                                                                                                   ggggTATCTTCAATGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                <  1:222634/246‑1 (MQ=255)
                                                                                                                                                                                                             caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCatcatc                                                                                                             >  1:796907/3‑178 (MQ=255)
                                                                                                                                                                                                             caaTGGTCCCCAGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCaaaaaaa                                    >  1:118863/3‑251 (MQ=255)
                                                                                                                                                                                                                tggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGAc                                 <  1:564395/243‑1 (MQ=255)
                                                                                                                                                                                                                 ggtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                <  1:569426/228‑1 (MQ=255)
                                                                                                                                                                                                                  gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGAt                                                                                                                                         >  1:567171/7‑145 (MQ=255)
                                                                                                                                                                                                                  gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACa                                                                                                        <  1:241233/172‑1 (MQ=255)
                                                                                                                                                                                                                  gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTaaaa                                                                        >  1:713014/7‑210 (MQ=255)
                                                                                                                                                                                                                  gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                               >  1:728520/7‑251 (MQ=255)
                                                                                                                                                                                                                  gtccccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACtt                               >  1:193704/7‑251 (MQ=255)
                                                                                                                                                                                                                      ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCATAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                           <  1:423158/249‑1 (MQ=255)
                                                                                                                                                                                                                      ccaGGCGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTc                           <  1:37453/249‑1 (MQ=255)
                                                                                                                                                                                                                           cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACACACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGtaataa                                                   >  1:441469/1‑222 (MQ=255)
                                                                                                                                                                                                                           cGTTCATGCAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGttt                      >  1:213533/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cAACGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCg              >  1:508718/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aCGGAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAgga                                                                                                                                                                 >  1:253923/1‑100 (MQ=255)
                                                                                                                                                                                                                                        gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATcc                                                >  1:381927/1‑212 (MQ=255)
                                                                                                                                                                                                                                        gAATATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa         >  1:148275/1‑251 (MQ=255)
                                                                                                                                                                                                                                         aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc        <  1:720757/251‑1 (MQ=255)
                                                                                                                                                                                                                                         aaTATAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATAc        <  1:235783/251‑1 (MQ=255)
                                                                                                                                                                                                                                          atatAATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATc                                                 <  1:5820/209‑1 (MQ=255)
                                                                                                                                                                                                                                           tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATa         >  1:472662/1‑248 (MQ=255)
                                                                                                                                                                                                                                           tataATCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTg      <  1:665791/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aTCGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATgg  <  1:529214/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGACTAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTTTTCCCGCAAATGACCGCTTGTTTAAGGAATTCCGCATAATCACCCTTATGATAAATTTCCAGTAGTGTCCAGtca                                                                                                                  >  1:560036/1‑137 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
CCGTTATGCCCGGAAGAAACCACATTGATAAAGTCATAAGTTTCCCCACCGCGTAATTCCGCCAGTAAAATACGATCCGGTTTCATTCGTAGGCAAGATTTCAGCAAAGAGGCAGCCGTGATTAAATCACCATTTTTTGCTTCGCTTGGATAGAATAAGTGGAAATAATTCTTATGTTGGCGTAAGAATAATTCCGGAGTATCTTCGATGGTGCCTAGGCGTTCATGCAACGGAATATAATCGACCAGGGCTTTCATTAAAGTGGTTTTCCCGGAGCCGGTTTCCCCGGCAATCACAATATTCTTCCCGCAGATAACCGCTTGTTTAAGAAATTCCGCATAATCGCCCTTATGATAAATTTCCAGTAGTGTCCGGTCATCATCTGACAAACGATTTTCCGTCTTGATAACACGACTAAAAAAGCCGTTGTTGATGTAATAATCCAGGTCAAAAAAAGACTTATTCGGTTTTCGCACCGTAATACTGATGG  >  1/1135928‑1136417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑