Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,592	A→G	I35I (ATT→ATC)	unknown ←	Type IV secretion system protein VirD4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,592	0	A	G	100.0%	211.6 / NA	77	I35I (ATT→ATC)	unknown	Type IV secretion system protein VirD4
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/37);  total (40/37)

TTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAACGG  >  1/1135406‑1135819                                                                                                                                                                                            |                                                                                                                                                                                                                                    tttttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg                                                                                                                                                                     <  1:878219/229‑1 (MQ=255)    ttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGa                                                                                                                                                                  >  1:339015/20‑251 (MQ=255)      gtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACa                                                                                                                                                                >  1:377257/18‑251 (MQ=255)         cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACaaa                                                                                                                                                              >  1:175940/15‑250 (MQ=255)         cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc                                                                                                                                                             <  1:102095/237‑1 (MQ=255)         cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCCCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc                                                                                                                                                             <  1:438044/237‑1 (MQ=255)                 tttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                                                                                     <  1:820065/245‑1 (MQ=255)                     tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                                                                                  <  1:724521/248‑1 (MQ=255)                     tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                                                                                  <  1:492742/248‑1 (MQ=255)                         aaaTTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAatat                                                                                                                                             <  1:106068/251‑1 (MQ=255)                                 cGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTACGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCtttt                                                                                                                                     >  1:275186/1‑251 (MQ=255)                                     accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                                                                                     <  1:322697/218‑1 (MQ=255)                                     accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                                                                                 >  1:665924/14‑251 (MQ=255)                                     accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                                                                                 >  1:504395/14‑251 (MQ=255)                                     accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                                                                                 >  1:342106/14‑251 (MQ=255)                                                 ttttttATGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                                                                                     >  1:839431/2‑219 (MQ=255)                                                 ttttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTcatcat                                                                                                                             >  1:785354/2‑243 (MQ=255)                                                  tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGaaaa                                                                                                                                                                                     <  1:160864/186‑1 (MQ=255)                                                  tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACg                                                                                                                                                                                 >  1:802996/1‑190 (MQ=255)                                                   ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCGCTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                                                                                   <  1:679974/251‑1 (MQ=255)                                                   ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                                                                                   <  1:248688/251‑1 (MQ=255)                                                        ggttGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                                                                                  >  1:147112/4‑215 (MQ=255)                                                           tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACtt                                                                                                                                                                                           <  1:294301/171‑1 (MQ=255)                                                           tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGaa                                                                                                           >  1:476304/1‑251 (MQ=255)                                                             aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAATTGGCATCTAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTaa                                                                                                                                                                                                     >  1:388841/1‑159 (MQ=255)                                                             aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg                                                                                                                                                                     >  1:797784/1‑191 (MQ=255)                                                                                              ctacaaaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCg                                                                         >  1:486176/24‑251 (MQ=255)                                                                                              ctacaaaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCg                                                                         >  1:392351/24‑251 (MQ=255)                                                                                                   aaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtg                                                                    <  1:786281/233‑1 (MQ=255)                                                                                                   aaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATCAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtg                                                                    <  1:463098/233‑1 (MQ=255)                                                                                                     actaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAg                                                                                <  1:314391/221‑1 (MQ=255)                                                                                                     actaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCCTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATACAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtgtg                                                                  >  1:443342/17‑251 (MQ=255)                                                                                                      ctaatatggatagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATgtgt                                                                 >  1:869315/16‑251 (MQ=255)                                                                                                      ctaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAatat                                                                                                                                             <  1:229109/160‑1 (MQ=255)                                                                                                      ctaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTcatcat                                                                                                                             >  1:326821/16‑191 (MQ=255)                                                                                                        aataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAac                                                                                                                         >  1:317060/14‑193 (MQ=255)                                                                                                            aggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg                                                           >  1:543871/10‑251 (MQ=255)                                                                                                             ggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCt                                                                                                                                        >  1:120654/9‑173 (MQ=255)                                                                                                             ggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGAtt                                                                             >  1:276380/9‑232 (MQ=255)                                                                                                              gagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg                                                           <  1:344569/242‑1 (MQ=255)                                                                                                              gagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg                                                           <  1:318898/242‑1 (MQ=255)                                                                                                                      gCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                                                                                   >  1:93268/1‑185 (MQ=255)                                                                                                                       ccataTTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGAc                                                 <  1:642513/246‑1 (MQ=255)                                                                                                                         ataTTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGt                                              <  1:361354/249‑1 (MQ=255)                                                                                                                           aTTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTg                                                                                                                                           >  1:220848/1‑156 (MQ=255)                                                                                                                           aTTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGAc                                                 <  1:782245/246‑1 (MQ=255)                                                                                                                            ttttCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTgg                                           <  1:320157/251‑1 (MQ=255)                                                                                                                              ttCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTa                                         >  1:581010/1‑251 (MQ=255)                                                                                                                                cggataattaccccaaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAAc                                       <  1:30557/235‑1 (MQ=255)                                                                                                                                  gataattaccccaaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACa                                      <  1:161612/236‑1 (MQ=255)                                                                                                                                    taattaccccaaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGa                                   >  1:9967/13‑251 (MQ=255)                                                                                                                                          ccccaaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCt                                                                                                                                        <  1:521336/138‑1 (MQ=255)                                                                                                                                           cccaaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACaataa                            <  1:177848/246‑1 (MQ=255)                                                                                                                                              aaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAatat                         <  1:751286/249‑1 (MQ=255)                                                                                                                                              aaaTGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAatat                         >  1:253896/3‑251 (MQ=255)                                                                                                                                                    cATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCg                   <  1:597030/251‑1 (MQ=255)                                                                                                                                                    cATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCg                   <  1:532029/251‑1 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtgtg                                                                  >  1:82953/1‑198 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtgtg                                                                  <  1:900208/198‑1 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACaataa                            >  1:712633/1‑236 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACaat                              <  1:207709/234‑1 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACaat                              >  1:415719/1‑234 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTttgtt             >  1:160197/1‑251 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTttgtt             >  1:78874/1‑251 (MQ=255)                                                                                                                                                          gCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTttgtt             >  1:316111/1‑251 (MQ=255)                                                                                                                                                           cgttagagTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTttgttg            <  1:428028/247‑1 (MQ=255)                                                                                                                                                           cgttagagTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTttgttg            <  1:9114/247‑1 (MQ=255)                                                                                                                                                             ttagagTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCg                   >  1:119886/3‑242 (MQ=255)                                                                                                                                                                gagTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGtaata       >  1:220782/1‑251 (MQ=255)                                                                                                                                                                  gTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAatat                         <  1:286458/231‑1 (MQ=255)                                                                                                                                                                   tAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAAc    <  1:97979/251‑1 (MQ=255)                                                                                                                                                                     gggTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGaa                                                                                                                                                                                       >  1:890207/1‑70 (MQ=255)                                                                                                                                                                     gggTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                                                                                   <  1:592138/138‑1 (MQ=255)                                                                                                                                                                     gggTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                                                                                   <  1:531420/138‑1 (MQ=255)                                                                                                                                                                     gggTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAACgg  >  1:825594/1‑251 (MQ=255)                                                                                                                                                                     gggTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAACgg  >  1:245743/1‑251 (MQ=255)                                                                                                                                                                       gtagtaGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAAc    >  1:806219/1‑247 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                    TTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCGTGTTCATGACTGTTGGTAACATGAACAATAATATCTATCGTTTGTTGTAATAAACGG  >  1/1135406‑1135819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑