Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,526	G→T	I57I (ATC→ATA)	unknown ←	Type IV secretion system protein VirD4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,526	0	G	T	100.0%	117.8 / NA	44	I57I (ATC→ATA)	unknown	Type IV secretion system protein VirD4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/18);  total (26/18)

CGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCG  >  1/1135288‑1135762                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa                                                                                                                                                                                                                                  >  1:637904/1‑251 (MQ=255) cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa                                                                                                                                                                                                                                  >  1:561656/1‑251 (MQ=255)                                                                                                                       tttttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg                                                                                                            <  1:878219/229‑1 (MQ=255)                                                                                                                          ttgtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGa                                                                                                         >  1:339015/20‑251 (MQ=255)                                                                                                                            gtacttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACa                                                                                                       >  1:377257/18‑251 (MQ=255)                                                                                                                               cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACaaa                                                                                                     >  1:175940/15‑250 (MQ=255)                                                                                                                               cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc                                                                                                    <  1:102095/237‑1 (MQ=255)                                                                                                                               cttctgattttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCCCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAAc                                                                                                    <  1:438044/237‑1 (MQ=255)                                                                                                                                       tttgtggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                            <  1:820065/245‑1 (MQ=255)                                                                                                                                           tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                         <  1:724521/248‑1 (MQ=255)                                                                                                                                           tggCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                         <  1:492742/248‑1 (MQ=255)                                                                                                                                               aaaTTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAatat                                                                                    <  1:106068/251‑1 (MQ=255)                                                                                                                                                       cGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTACGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCtttt                                                                            >  1:275186/1‑251 (MQ=255)                                                                                                                                                           accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                            <  1:322697/218‑1 (MQ=255)                                                                                                                                                           accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                        >  1:665924/14‑251 (MQ=255)                                                                                                                                                           accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                        >  1:342106/14‑251 (MQ=255)                                                                                                                                                           accataaagggattttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTtca                                                                        >  1:504395/14‑251 (MQ=255)                                                                                                                                                                       ttttttATGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGaataa                                                                                            >  1:839431/2‑219 (MQ=255)                                                                                                                                                                       ttttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTcatcat                                                                    >  1:785354/2‑243 (MQ=255)                                                                                                                                                                        tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGaaaa                                                                                                                            <  1:160864/186‑1 (MQ=255)                                                                                                                                                                        tttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACg                                                                                                                        >  1:802996/1‑190 (MQ=255)                                                                                                                                                                         ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCGCTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                          <  1:679974/251‑1 (MQ=255)                                                                                                                                                                         ttttAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                          <  1:248688/251‑1 (MQ=255)                                                                                                                                                                              ggttGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGc                                                                                         >  1:147112/4‑215 (MQ=255)                                                                                                                                                                                 tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACtt                                                                                                                                  <  1:294301/171‑1 (MQ=255)                                                                                                                                                                                 tGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGaa                                                  >  1:476304/1‑251 (MQ=255)                                                                                                                                                                                   aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAATTGGCATCTAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTaa                                                                                                                                            >  1:388841/1‑159 (MQ=255)                                                                                                                                                                                   aTAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAg                                                                                                            >  1:797784/1‑191 (MQ=255)                                                                                                                                                                                                                    ctacaaaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCg                >  1:392351/24‑251 (MQ=255)                                                                                                                                                                                                                    ctacaaaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCg                >  1:486176/24‑251 (MQ=255)                                                                                                                                                                                                                         aaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtg           <  1:786281/233‑1 (MQ=255)                                                                                                                                                                                                                         aaactaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATCAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtg           <  1:463098/233‑1 (MQ=255)                                                                                                                                                                                                                           actaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAg                       <  1:314391/221‑1 (MQ=255)                                                                                                                                                                                                                           actaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCCTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATACAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCAtgtg         >  1:443342/17‑251 (MQ=255)                                                                                                                                                                                                                            ctaatatggatagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATgtgt        >  1:869315/16‑251 (MQ=255)                                                                                                                                                                                                                            ctaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAatat                                                                                    <  1:229109/160‑1 (MQ=255)                                                                                                                                                                                                                            ctaataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTcatcat                                                                    >  1:326821/16‑191 (MQ=255)                                                                                                                                                                                                                              aataaggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAac                                                                >  1:317060/14‑193 (MQ=255)                                                                                                                                                                                                                                  aggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg  >  1:543871/10‑251 (MQ=255)                                                                                                                                                                                                                                   ggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCt                                                                               >  1:120654/9‑173 (MQ=255)                                                                                                                                                                                                                                   ggagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGAtt                    >  1:276380/9‑232 (MQ=255)                                                                                                                                                                                                                                    gagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg  <  1:318898/242‑1 (MQ=255)                                                                                                                                                                                                                                    gagagactGCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCg  <  1:344569/242‑1 (MQ=255)                                                                                                                                                                                                                                            gCCATATTTTCGGATAATTACCCCAAATGGCATCCAGCGTTAGAGTAGGGTAGTAGCGCTTAAATGCTTCGATTAAACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGGAATAATGCAATATTGGCTTTTTTCATCATTAacctacct                                                          >  1:93268/1‑185 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             CGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAATTTCCCAGGATGGCATCCAGCGTAAGAGTAGGGTAGTAACGCTTGAACGCTTCAATCAGACGAATTTTATTTGCCAGTAGAAGTAAAAGCCCACTTAGAAAATACGTCGGAAGCAAAGTGACAAACAGAAATAATGCAATATTGGCTTTTTTCATCATTAACCTACCTTGAGCGAATTCAGTTTAGCTTGCGGATCAAAATAGATTTCCGTCATGTGTCTGCCG  >  1/1135288‑1135762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑