Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,325	G→A	F124F (TTC→TTT)	unknown ←	Type IV secretion system protein VirD4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,325	0	G	A	100.0%	144.1 / NA	54	F124F (TTC→TTT)	unknown	Type IV secretion system protein VirD4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

CGCATATCAATACAATCCAATGGATTAAATCGGTGCGTGTTACGGTCAAACGGGGCAAAGCGATAGACTTTTTGCCCCAATATTTTTTGACGGTATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAA  >  1/1135104‑1135538                                                                                                                                                                                                                              |                                                                                                                                                                                                                       cgcATATCAATACAATCCAGCGGACTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagttt                                                                                                                                                                                           <  1:392692/251‑13 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATGACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttatta                                                                                                                                                                                       >  1:426273/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttatta                                                                                                                                                                                       >  1:827872/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttatta                                                                                                                                                                                       >  1:558210/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttatta                                                                                                                                                                                       >  1:341298/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttatta                                                                                                                                                                                       >  1:345639/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaac                                                                                                                                                                                                     <  1:791628/237‑3 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaac                                                                                                                                                                                                     >  1:590676/1‑235 (MQ=255)     tatCAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaac                                                                                                                                                                                                     <  1:231387/237‑3 (MQ=255)        cAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattaccg                                                                                                                                                                                    >  1:674293/1‑232 (MQ=255)        cAATACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGCTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattaccg                                                                                                                                                                                    <  1:673921/251‑20 (MQ=255)          aTACAATCCAGCGGATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattaccgag                                                                                                                                                                                  >  1:225216/1‑230 (MQ=255)                       gATTAAACCGGTGCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATa                                                                                                                                                                     >  1:281345/1‑251 (MQ=255)                                   gCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattac                                                                                                                                                                                      <  1:90083/222‑18 (MQ=255)                                   gCGTATTACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaac                                                                                                                                                                                                     >  1:495215/1‑205 (MQ=255)                                     gtattACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattaccg                                                                                                                                                                                    >  1:551262/4‑203 (MQ=255)                                      tattACGGTCAAACGGGGCAAAGCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGAATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGAAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGttttttt                                                                                                                                                        >  1:611429/3‑246 (MQ=255)                                                            gCGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaac                                                                                                                                                                                                     <  1:123490/182‑3 (MQ=255)                                                             cGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtac                                                                                                                               <  1:410405/251‑8 (MQ=255)                                                             cGATATACCTTTTGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgta                                                                                                                                >  1:71111/1‑244 (MQ=255)                                                               atataccttttGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtactt                                                                                                                             >  1:877799/8‑242 (MQ=255)                                                                    ccttttGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgat                                                                                                                        <  1:163853/249‑15 (MQ=255)                                                                     cttttGCCCTAATATTTTTTGTCGATATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagcagtttattac                                                                                                                                                                                      <  1:469414/187‑18 (MQ=255)                                                                                               aTTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgattttgtgg                                                                                                                 >  1:761182/1‑210 (MQ=255)                                                                                                 ttACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATaaaacggagc                                                                                                                                                                                                <  1:624862/150‑8 (MQ=255)                                                                                                                                 gTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa                                                           >  1:332126/1‑251 (MQ=255)                                                                                                                                  tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa                                                           <  1:874140/250‑1 (MQ=255)                                                                                                                                  tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa                                                           <  1:173188/250‑1 (MQ=255)                                                                                                                                  tCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa                                                           <  1:53841/250‑1 (MQ=255)                                                                                                                                          aaCCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTa                                                  <  1:617744/251‑1 (MQ=255)                                                                                                                                           aCCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa                                                 >  1:185175/1‑250 (MQ=255)                                                                                                                                                aGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctc                                             <  1:459109/250‑6 (MQ=255)                                                                                                                                                 gATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCa                                                           <  1:205277/235‑1 (MQ=255)                                                                                                                                                  aTTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctc                                             <  1:118435/248‑6 (MQ=255)                                                                                                                                                     gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCAttttttgtacttctgatt                                                                                                                       >  1:126125/1‑156 (MQ=255)                                                                                                                                                     gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactaca                                       >  1:801555/1‑240 (MQ=255)                                                                                                                                                     gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactaca                                       >  1:271261/1‑240 (MQ=255)                                                                                                                                                     gTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTa                                                  <  1:830119/240‑1 (MQ=255)                                                                                                                                                       tCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTGCCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactacaaa                                     >  1:640863/1‑238 (MQ=255)                                                                                                                                                       tCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactac                                        <  1:495319/248‑11 (MQ=255)                                                                                                                                                         ccATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcactacaaaac                                   <  1:89452/251‑16 (MQ=255)                                                                                                                                                                       ttGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagac                     <  1:397716/251‑11 (MQ=255)                                                                                                                                                                             tAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccat               >  1:213856/1‑235 (MQ=255)                                                                                                                                                                                  aTCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagc                                               <  1:806579/214‑4 (MQ=255)                                                                                                                                                                                   tCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccatatttt          >  1:37004/1‑229 (MQ=255)                                                                                                                                                                                   tCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggagagactgccatatttt          >  1:30421/1‑229 (MQ=255)                                                                                                                                                                                         cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaaggag                         <  1:665929/229‑7 (MQ=255)                                                                                                                                                                                         cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa  >  1:637904/1‑251 (MQ=255)                                                                                                                                                                                         cGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAT‑AAGGAGAGACTGCCATATTTTCGGATaa  >  1:561656/1‑251 (MQ=255)                                                                                                                                                                                          gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTtttttcttc                                                                                                                                                    >  1:381531/1‑105 (MQ=255)                                                                                                                                                                                          gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaagctcac                                           <  1:703339/210‑8 (MQ=255)                                                                                                                                                                                          gCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTAAGCTCACTACAAAACTAAtaagg                           <  1:689161/226‑5 (MQ=255)                                                                                                                                                                                             tttACCTCCCCGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTTTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa                                                 >  1:783674/1‑200 (MQ=255)                                                                                                                                                                                                    ccccGTGTCGGCGCTGCCAGTGAAACAAATTGCGCACCGATAAAACGGAGCAGTTTATTACCGAGTTTGCCGACAATAATCCCGTCTTTTTCTTCCAATAAACCCATTTTTTGTACTTCTGATTTTGTGGCAAATTCAGCGTTACCATAAAGGGATTTTTTAGGTTGATAGAATAGTTTCAGCATGATTAGTaa                                                 >  1:643501/1‑193 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                       CGCATATCAATACAATCCAATGGATTAAATCGGTGCGTGTTACGGTCAAACGGGGCAAAGCGATAGACTTTTTGCCCCAATATTTTTTGACGGTATTTACTGGTGATACGATAGCATTCGTTTTTAATGTCCTTCACAACCACAGATTGTTCCCATGCCAGCAAATTTGGAATAACAATCCCTACGCTTTTACCTCCCCGTGTCGGCGCTGCCAGTGAAACGAATTGCGCACCGATAAAGCGTAAAAGTTTTTTGCCGAGCTTGCCGACGATAATGCCGTTTTCTTCTTCCATCAAGCCCATTTCCCGCGCTTCCGAGGCAGTCGCAAATTCGGCGTTACCATAAAGATTCTTTTTAGGCTGATAGAAGAGTTTCAGCATAATCAGTAGGCAGACGACAAAACTAATCAATAAG‑GATTGCCAGATTTTCGGATAA  >  1/1135104‑1135538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑