Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,134,188	C→T	P503P (CCG→CCA)	unknown ←	Type IV secretion system protein VirD4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,134,188	0	C	T	100.0%	220.3 / NA	78	P503P (CCG→CCA)	unknown	Type IV secretion system protein VirD4
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/35);  total (43/35)

TGGTCGGCATAAGGATCGTTGTAGTACACCGCCTTATTACACAGTATCGGTTTACTGTTGTTTAACATCACCACTTCTTTAAATTCTCCGATTAGTCTAAATTCCTGTGGTAAAAGCAACGGTCGCCCATGGTCGGTAATACTGTGACTGGACGTTTTTCCGCGACTATGGCTTAATTTATCTACAGTACGATTGCCCAGCACTTTTGAATATTCTTCCGCGTCCTCCTGATTGTCGGGGGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTTGTGATTGATAAATAATCAAT  >  1/1134069‑1134419                                                                                                                        |                                                                                                                                                                                                                                        tctataaaaTAAGGATCGTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAAt                                                                                                      <  1:420535/243‑1 (MQ=255)  ctataaaaTAAGGATCGTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        <  1:200735/177‑1 (MQ=255)    ataaaaTAAGGATCGTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAat                                                                                                                                              <  1:659792/203‑1 (MQ=255)     taaaaTAAGGATCGTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGttttacct                                                                                                                                                                                               >  1:114465/5‑154 (MQ=255)             ggATCGTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGat                                                                                          >  1:769815/1‑251 (MQ=255)                  gTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:848076/1‑251 (MQ=255)                  gTTGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:530609/1‑251 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATAc                                                                                                                                                                                                                   >  1:760081/1‑124 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTAt                                                                                                                                                                                        <  1:185440/151‑1 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        <  1:790102/167‑1 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATAttcttctg                                                                                                                                     <  1:464836/202‑3 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTggtt                                                                                                                        >  1:684656/1‑212 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAg                                                                                                         >  1:858786/1‑230 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:718894/1‑250 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:103576/1‑250 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:37047/1‑250 (MQ=255)                   ttGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGAtattat                                                                                     >  1:749766/1‑250 (MQ=255)                    tGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATcc                                                                                   <  1:155298/251‑1 (MQ=255)                    tGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATcc                                                                                   <  1:624122/251‑1 (MQ=255)                    tGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATcc                                                                                   <  1:197858/251‑1 (MQ=255)                    tGTAATACACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATcc                                                                                   <  1:369443/251‑1 (MQ=255)                     gtaataCACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTGCCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAAt                                                                                                      <  1:287737/227‑1 (MQ=255)                     gtaataCACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        >  1:795909/5‑165 (MQ=255)                     gtaataCACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTgg                                                                                                                          <  1:79394/207‑2 (MQ=255)                     gtaataCACTGCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCa                                                                                  >  1:371636/5‑251 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGTGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAatat                                                                                                                                            <  1:514593/183‑1 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCtt                                                                                                                                                                                   >  1:734341/1‑144 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        <  1:308318/155‑1 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        >  1:491100/1‑155 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGAt                                                                              >  1:894013/1‑245 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCttt                                                                        >  1:569444/1‑251 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCttt                                                                        >  1:575570/1‑251 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCttt                                                                        >  1:48082/1‑251 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCttt                                                                        >  1:286263/1‑251 (MQ=255)                               gCCTTATTACATATAATCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAACACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCttt                                                                        >  1:451822/1‑251 (MQ=255)                                    attacatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCa                                                                    >  1:371289/11‑250 (MQ=255)                                    attacatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCa                                                                    >  1:309138/11‑250 (MQ=255)                                    attacatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTATGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCa                                                                    >  1:486228/11‑250 (MQ=255)                                    attacatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGAAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGCTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCa                                                                    >  1:424435/11‑250 (MQ=255)                                       acatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTAttt                                                                                                                                                                          <  1:378397/138‑3 (MQ=255)                                       acatataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCatat                                                                                                           >  1:894996/8‑208 (MQ=255)                                        catataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACttt                                                                                                                                                   <  1:595757/161‑1 (MQ=255)                                        catataaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAat                                                                                                                                              >  1:500270/7‑172 (MQ=255)                                            taaTCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTAttt                                                                                                                                                                          >  1:23303/3‑138 (MQ=255)                                               tCGGTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCg                                                        <  1:314932/251‑1 (MQ=255)                                                  gTTTGCTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTAc                                                                                                 <  1:7716/207‑1 (MQ=255)                                                      gcTGTTATTTAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCg                                                                                            >  1:254296/2‑208 (MQ=255)                                                         gttatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAg                                              >  1:309491/5‑251 (MQ=255)                                                         gttatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAg                                              >  1:341106/5‑251 (MQ=255)                                                         gttatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAg                                              >  1:578807/5‑251 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAg                                                                                                         >  1:327104/3‑190 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:32122/249‑1 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:625294/249‑1 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:688951/249‑1 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:186147/249‑1 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:381475/249‑1 (MQ=255)                                                           tatttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGc                                            <  1:752253/249‑1 (MQ=255)                                                            atttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCAc                                                                                                                                                      <  1:805268/143‑1 (MQ=255)                                                            atttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATAttcttc                                                                                                                                       <  1:36872/158‑1 (MQ=255)                                                            atttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGatat                                                                                        <  1:568855/205‑1 (MQ=255)                                                            atttAACATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGGAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCAc                                                                   <  1:434323/226‑1 (MQ=255)                                                                aaCATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTAt                                        >  1:415687/1‑250 (MQ=255)                                                                aaCATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATc                                       <  1:412564/251‑1 (MQ=255)                                                                  cATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGttttacct                                                                                                                                                                                               >  1:796441/1‑93 (MQ=255)                                                                  cATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGAt                                                                              <  1:91508/210‑1 (MQ=255)                                                                  cATCACCACTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCgg                                     >  1:264383/1‑251 (MQ=255)                                                                       ccacTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        >  1:205329/1‑115 (MQ=255)                                                                       ccacTTCTTTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTAc                                                                                                                                                                        >  1:825948/1‑115 (MQ=255)                                                                             cttTAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGa                                                                               >  1:826163/1‑198 (MQ=255)                                                                               ttAAATTCGCCGATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGccc                        <  1:40228/251‑1 (MQ=255)                                                                                          gATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCt                                                                          <  1:178008/190‑1 (MQ=255)                                                                                          gATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTtgtg                   <  1:492632/245‑1 (MQ=255)                                                                                          gATTAATCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTTGtgattgat             >  1:881551/1‑251 (MQ=255)                                                                                               atCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGAc                                                                                                                                                                                           >  1:227709/2‑72 (MQ=255)                                                                                               atCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAatat                                                                                                                                            <  1:228695/118‑1 (MQ=255)                                                                                               atCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCatat                                                                                                           <  1:677598/151‑1 (MQ=255)                                                                                               atCTAAACTCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTTGTGATTGataaata        >  1:185774/2‑251 (MQ=255)                                                                                                     actCCTGCGGTAAAAGTAATGGTCGCCCATGGTCGGTAATACTGTGACTGGATGTTTTACCTCGACTATGGCTTAATTTATTTACAGTGCGATTACCTAGCACTTTAGAATATTCTTCTGCGTCCTCCTGGTTGTCGGGTGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTTGTGATTGATAAATAATCAAt  >  1:68808/3‑251 (MQ=255)                                                                                                                        |                                                                                                                                                                                                                                        TGGTCGGCATAAGGATCGTTGTAGTACACCGCCTTATTACACAGTATCGGTTTACTGTTGTTTAACATCACCACTTCTTTAAATTCTCCGATTAGTCTAAATTCCTGTGGTAAAAGCAACGGTCGCCCATGGTCGGTAATACTGTGACTGGACGTTTTTCCGCGACTATGGCTTAATTTATCTACAGTACGATTGCCCAGCACTTTTGAATATTCTTCCGCGTCCTCCTGATTGTCGGGGGCATATAGAATTTTACAGGCGATATTATCCAGGATAGCTTTAGCACCATCTTTACCGTAACCATTAGGCTTATCGGCACGAAGTTGCCCTTGTGATTGATAAATAATCAAT  >  1/1134069‑1134419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑