Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,124,505	C→A	G181C (GGT→TGT)	unknown ←	Outer membrane receptor for lactoferrin or transferrin, TonB‑dependent protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,124,505	0	C	A	100.0%	36.3 / NA	15	G181C (GGT→TGT)	unknown	Outer membrane receptor for lactoferrin or transferrin, TonB‑dependent protein A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/5);  total (10/5)

GATATATGCAGGTAATGTCATATCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACCGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAACCCCCAGTTTT  >  1/1124257‑1124750                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gATATATGCAGGTAATGTCATATCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcgg                                                                                                                                                                                                                                                     >  1:539473/1‑251 (MQ=255)   tatatGCAGGTAATGTCATATCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggac                                                                                                                                                                                                                                                   <  1:320720/251‑1 (MQ=255)             tAATGTCATATCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacgtgttgccg                                                                                                                                                                                                                                          <  1:305776/250‑9 (MQ=255)                      atCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacgtgttgccgaccgcgagat                                                                                                                                                                                                                                <  1:596900/251‑19 (MQ=255)                                          gTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacgtgttgccgac                                                                                                                                                                                                                                        <  1:84937/223‑11 (MQ=255)                                          gTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacgtgttgc                                                                                                                                                                                                                                            >  1:445417/1‑213 (MQ=255)                                               gTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacgtgttgccgac                                                                                                                                                                                                                                        >  1:820994/1‑208 (MQ=255)                                                              gCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacg                                                                                                                                                                                                                                                  <  1:557833/193‑1 (MQ=255)                                                                                                       gAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcg                                                                                                                                                                                                                                                      <  1:369503/148‑1 (MQ=255)                                                                                                                                                                                                     ggAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACcggacg                                                                                                                                                                                                                                                  <  1:497607/58‑1 (MQ=255)                                                                                                                                                                                                                                 ttctaatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAAtgtg                                                        >  1:121354/21‑216 (MQ=255)                                                                                                                                                                                                                                 ttctaatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTa                     >  1:79751/21‑251 (MQ=255)                                                                                                                                                                                                                                 ttctaatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTa                     >  1:593489/21‑251 (MQ=255)                                                                                                                                                                                                                                 ttctaatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTa                     >  1:741437/21‑251 (MQ=255)                                                                                                                                                                                                                                    taatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTc                  <  1:817213/234‑1 (MQ=255)                                                                                                                                                                                                                                    taatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTc                  <  1:44615/234‑1 (MQ=255)                                                                                                                                                                                                                                    taatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTc                  <  1:729429/234‑1 (MQ=255)                                                                                                                                                                                                                                     aatattaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAg                                                  <  1:663501/202‑1 (MQ=255)                                                                                                                                                                                                                                        attaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATg                                                                                                         <  1:508716/147‑1 (MQ=255)                                                                                                                                                                                                                                        attaagtggttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTc                  >  1:82989/14‑247 (MQ=255)                                                                                                                                                                                                                                                gttggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGAACCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAAc            >  1:237534/6‑245 (MQ=255)                                                                                                                                                                                                                                                  tggttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAACCCCCAGtt    >  1:532549/4‑251 (MQ=255)                                                                                                                                                                                                                                                    gttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGc                            >  1:590650/2‑225 (MQ=255)                                                                                                                                                                                                                                                    gttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAAc            >  1:356418/2‑241 (MQ=255)                                                                                                                                                                                                                                                    gttACAGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAACCCCCAGtttt  >  1:696615/2‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GATATATGCAGGTAATGTCATATCGCGAATATCAAAGCGCTGTTTAGTATATTCCATAACCGCCCCGAGATAATGTTGCTCGGAAAAGTGATAACCCGGGCGGAAAAACCAAGATTGACTACGAGATTTCATCGGGTTCGGCGCAACACGATTCTCACCGGTATACTCTCCCTGACTAGCCGTCTCTTCTTGCTCAGGAATAACTCTTGCCAGTGGCTTTATGTAACCTTTGCATAAATCAACATTACCGGACGGACAATCCTCTTGCAAAAGAAAATAGTTATATGTACCGCCCGTACCCGGTACGGCAACCTGGCGGGAATAACGCTGTCTTCCCCTGCGTGCATCACGATGAATTTGTGCTTCTTTGCCTCGACGATCCGTGTAGATGAATAGACCCTCAAACCCATCGTTTTTTCCCGCTATTGCAATGGAATGTGTGGAAGCACGATTTTTACTGGCATAGGCGTTCTTAGTCTGTAACCCCCAGTTTT  >  1/1124257‑1124750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑