Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,106,550	T→C	S70G (AGC→GGC)	unknown ←	Phenylalanyl‑tRNA synthetase beta chain (EC 6.1.1.20)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,106,550	0	T	C	100.0%	229.4 / NA	81	S70G (AGC→GGC)	unknown	Phenylalanyl‑tRNA synthetase beta chain (EC 6.1.1.20)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/36);  total (45/36)

TCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCTCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAATTATTTA  >  1/1106304‑1106783                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          tCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCcaca                                                                                                                                                                                                                                       <  1:349660/251‑1 (MQ=255)        atCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGCCCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                                                <  1:394851/251‑1 (MQ=255)        atCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                                                <  1:689358/251‑1 (MQ=255)          cACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAATAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTg                                                                                                                                                                                                                              >  1:250229/1‑251 (MQ=255)          cACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTg                                                                                                                                                                                                                              >  1:412561/1‑251 (MQ=255)            cGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTgg                                                                                                                                                                                                                             >  1:760688/1‑250 (MQ=255)            cGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGt                                                                                                                                                                                                                            >  1:605050/1‑251 (MQ=255)                           aTCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATc                                                                                                                                                                                                             <  1:258484/251‑1 (MQ=255)                                   gTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCg                                                                                                                                                                                                     >  1:385832/1‑251 (MQ=255)                                   gTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCg                                                                                                                                                                                                     >  1:693895/1‑251 (MQ=255)                                    tCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCgg                                                                                                                                                                                                    >  1:482376/1‑251 (MQ=255)                                    tCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCgg                                                                                                                                                                                                    >  1:96607/1‑251 (MQ=255)                                      ttGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGgt                                                                                                                                                                                                  >  1:5112/1‑251 (MQ=255)                                                 cGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCACGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATc                                                                                                                                                                                                             <  1:779870/229‑1 (MQ=255)                                                   aTAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCAtt                                                                                                                                                                                      >  1:168397/1‑250 (MQ=255)                                                   aTAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCAtt                                                                                                                                                                                      >  1:358327/1‑250 (MQ=255)                                                        gCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAAtt                                                                                                                                                                                                                 >  1:505466/1‑218 (MQ=255)                                                            ttAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACtt                                                                                                                                                                             <  1:303822/250‑1 (MQ=255)                                                                      aTTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                                   <  1:657638/250‑1 (MQ=255)                                                                         cAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACg                                                                                                                                                               >  1:323042/1‑251 (MQ=255)                                                                           aTACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                                   <  1:469331/245‑1 (MQ=255)                                                                               cGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTg                                                                                                                                                         <  1:203748/251‑1 (MQ=255)                                                                                   ttCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGaaa                                                                                                                                                      <  1:389523/250‑1 (MQ=255)                                                                                         gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                                               >  1:119337/1‑251 (MQ=255)                                                                                         gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                                               >  1:657933/1‑251 (MQ=255)                                                                                         gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                                               >  1:758767/1‑251 (MQ=255)                                                                                           atgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                                                <  1:852923/168‑1 (MQ=255)                                                                                               aCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAAc                                                                                                                                           >  1:466060/1‑249 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                                                >  1:652210/1‑161 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTAt                                                                                                                                                                                                              <  1:535466/179‑1 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                                   >  1:724743/1‑222 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                                      >  1:771776/1‑251 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                                      >  1:859613/1‑251 (MQ=255)                                                                                                  gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                                      >  1:366227/1‑251 (MQ=255)                                                                                                         ccTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCcac                                                                                                                                  >  1:352951/1‑248 (MQ=255)                                                                                                            tCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGt                                                                                                                             >  1:676854/1‑250 (MQ=255)                                                                                                                       cccGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAAc                                                                                                                 <  1:453527/250‑1 (MQ=255)                                                                                                                          gcgTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCa                                                                                                              >  1:371176/1‑251 (MQ=255)                                                                                                                              aaTTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACAt                                                                                                          >  1:762832/1‑251 (MQ=255)                                                                                                                              aaTTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACAt                                                                                                          <  1:838973/251‑1 (MQ=255)                                                                                                                                    tttttCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTaa                                                                                                     <  1:642432/249‑1 (MQ=255)                                                                                                                                    gTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAAt                                                                                                    >  1:386380/1‑251 (MQ=255)                                                                                                                                     ttttCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAAtt                                                                                                   <  1:564124/251‑1 (MQ=255)                                                                                                                                                 tGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGt                                                                                       <  1:409048/251‑1 (MQ=255)                                                                                                                                                 tGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGt                                                                                       <  1:834245/251‑1 (MQ=255)                                                                                                                                                    aaTTGCCCGGCAATTTTGCCCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGc                                                                                    <  1:333834/251‑1 (MQ=255)                                                                                                                                                      ttGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTc                                                                                  <  1:136265/251‑1 (MQ=255)                                                                                                                                                             gCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGaa                                                                           >  1:607838/1‑251 (MQ=255)                                                                                                                                                                        ccTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCTACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGAt                                                                 <  1:436983/250‑1 (MQ=255)                                                                                                                                                                          ttCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCTTTTCGCCGCACACAATATCCAATAAACTTTCGCCGCCCACATTTCCTTTGGTCACTCTTAATTTATCCTCATCCGGGTGTTGCGCGCATTCCACAACTTCGGCTCCCACAACGCCACCTAAATCGCCGGCACCCTTCTCCACACCGTCAACTTCCAGACCCAACGTGGTAATTTTATCGCAAAGCTTCTCCGTGGAAAGTACCGGATTaa                                                              >  1:611992/1‑251 (MQ=255)                                                                                                                                                                            ctcccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCcaccac                                                                                                                                                                               <  1:780115/133‑1 (MQ=255)                                                                                                                                                                            ctcccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAcc                                                            <  1:356651/248‑1 (MQ=255)                                                                                                                                                                            cTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAcc                                                            <  1:700983/251‑1 (MQ=255)                                                                                                                                                                             ccccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAccc                                                           <  1:857890/249‑1 (MQ=255)                                                                                                                                                                                    caAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACg                                                    <  1:17854/251‑1 (MQ=255)                                                                                                                                                                                      aGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTa                                                  >  1:562916/1‑251 (MQ=255)                                                                                                                                                                                           cTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCAt                                             >  1:834220/1‑251 (MQ=255)                                                                                                                                                                                            tttCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGCCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCAtt                                            >  1:158448/1‑251 (MQ=255)                                                                                                                                                                                                 aCCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCcaccac                                                                                                                                                                               <  1:641103/115‑1 (MQ=255)                                                                                                                                                                                                     ttGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTaa                                   <  1:9951/251‑1 (MQ=255)                                                                                                                                                                                                     ttGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTaa                                   <  1:270468/251‑1 (MQ=255)                                                                                                                                                                                                           gCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTc                              <  1:110631/250‑1 (MQ=255)                                                                                                                                                                                                           gCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATCCACGTACCCATTGTTCACTAAATTTCa                             >  1:96975/1‑251 (MQ=255)                                                                                                                                                                                                                      cgccgcACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTAtctc                   <  1:36987/250‑1 (MQ=255)                                                                                                                                                                                                                          gcACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGgt                                                                                                                                                                                                  <  1:423052/71‑1 (MQ=255)                                                                                                                                                                                                                          gcACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATAc                          >  1:461739/1‑239 (MQ=255)                                                                                                                                                                                                                            acacaATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTctcactca            >  1:514082/1‑251 (MQ=255)                                                                                                                                                                                                                             cacaATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTAc                                                 >  1:459499/1‑213 (MQ=255)                                                                                                                                                                                                                             cacaATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCaa           >  1:838319/1‑251 (MQ=255)                                                                                                                                                                                                                             cacaATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCaa           >  1:831238/1‑251 (MQ=255)                                                                                                                                                                                                                               caATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATcc                                                                                                                                                                                                            <  1:891239/56‑1 (MQ=255)                                                                                                                                                                                                                                 atatCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAatt       >  1:746622/1‑251 (MQ=255)                                                                                                                                                                                                                                 atatCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGCGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGt                                                   >  1:78933/1‑207 (MQ=255)                                                                                                                                                                                                                                   atCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTg                                           <  1:311795/213‑1 (MQ=255)                                                                                                                                                                                                                                   atCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCAc                                      >  1:262632/1‑218 (MQ=255)                                                                                                                                                                                                                                      cAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAAccc                                                                                                               <  1:656791/142‑1 (MQ=255)                                                                                                                                                                                                                                      cAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCAc                                                     >  1:259350/1‑200 (MQ=255)                                                                                                                                                                                                                                      cAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAATTATTTa  >  1:842065/1‑251 (MQ=255)                                                                                                                                                                                                                                      cAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAATTATTTa  >  1:856434/1‑251 (MQ=255)                                                                                                                                                                                                                                      cAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAATTATTTa  >  1:858776/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaTAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTAccc                                               <  1:536426/205‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          TCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCTCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTCTCACTCAAAATTATTTA  >  1/1106304‑1106783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑