Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,103,893	T→C	I41M (ATA→ATG)	unknown ←	Probable endopeptidase NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,103,893	0	T	C	100.0%	212.5 / NA	74	I41M (ATA→ATG)	unknown	Probable endopeptidase NlpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/44);  total (30/44)

CATGATAGCCATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCTATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGAACACCACCACACGA  >  1/1103646‑1104136                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    caTGATAGCCATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCcata                                                                                                                                                                                                                                                  >  1:641368/1‑251 (MQ=255) caTGATAGCCATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCcata                                                                                                                                                                                                                                                  <  1:795626/251‑1 (MQ=255)     agaGCCATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACCGGGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCataat                                                                                                                                                                                                                                                <  1:212767/248‑1 (MQ=255)         ccATGAGGACCGCGACCGGTTTTAAAGAACCCCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGAt                                                                                                                                                                                                                                           <  1:700811/250‑1 (MQ=255)         ccATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCCCC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGAt                                                                                                                                                                                                                                           <  1:121303/250‑1 (MQ=255)          cATGAGGCCCGCGACCGGTTTTAAAGAACACCAAATCCCC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCataat                                                                                                                                                                                                                                                <  1:635625/244‑1 (MQ=255)          cATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAg                                                                                                                                                                                                                                              >  1:521031/1‑246 (MQ=255)                 aCCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGTTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATc                                                                                                                                                                                                                                   <  1:185536/250‑1 (MQ=255)                 aCCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATc                                                                                                                                                                                                                                   <  1:655120/250‑1 (MQ=255)                      gACCGGTTTTAAAGAACCCCAAATCCCC‑GGTTTGAATTTCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGCCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGccc                                                                                                                                                                                                                              <  1:755740/250‑1 (MQ=255)                           gTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGta                                                                                                                                                                                                                        >  1:465481/1‑251 (MQ=255)                           gTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGta                                                                                                                                                                                                                        >  1:849125/1‑251 (MQ=255)                                    aaCACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATc                                                                                                                                                                                                                 <  1:590481/249‑1 (MQ=255)                                      cacCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCcgcg                                                                                                                                                                                                             >  1:109075/1‑251 (MQ=255)                                           aaTCCCC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCataat                                                                                                                                                                                                                                                <  1:646895/211‑1 (MQ=255)                                           aaTCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGAt                                                                                                                                                                                                                                           >  1:269341/1‑216 (MQ=255)                                                     tttGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATTTTGGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACAAAACGGGAGAAATCCCCGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACgg                                                                                                                                                                                                <  1:265086/250‑1 (MQ=255)                                                            atCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCgg                                                                                                                                                                                        <  1:68244/251‑1 (MQ=255)                                                               cccTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAAc                                                                                                                                                                                     <  1:53204/250‑1 (MQ=255)                                                                ccTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAAc                                                                                                                                                                                     <  1:266354/250‑1 (MQ=255)                                                                   ttttCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAgg                                                                                                                                                                                  >  1:75396/1‑250 (MQ=255)                                                                    tttCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGAt                                                                                                                                                                                                                                           <  1:850417/192‑1 (MQ=255)                                                                      tCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGAt                                                                                                                                                                                                                                           >  1:124525/1‑190 (MQ=255)                                                                      tCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAggcgg                                                                                                                                                                               >  1:803466/1‑250 (MQ=255)                                                                      tCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAggcgg                                                                                                                                                                               >  1:614626/1‑250 (MQ=255)                                                                      tCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGATCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGaa                                                                                                                                                                                      >  1:72827/1‑243 (MQ=255)                                                                      tCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCAATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAggcgg                                                                                                                                                                               >  1:203837/1‑250 (MQ=255)                                                                       aaaTATACTGCCCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCATTTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCt                                                                                                                                                                             <  1:527689/250‑1 (MQ=255)                                                                        aaTATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTa                                                                                                                                                                            >  1:324955/1‑251 (MQ=255)                                                                           ataCTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACg                                                                                                                                                                         >  1:103986/1‑251 (MQ=255)                                                                                gACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCATTTTTATGCCGAAGCGATCGAAAAGGGTGGTTTGCACGAAACGGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGAc                                                                                                                                                                                                  <  1:619678/221‑1 (MQ=255)                                                                                gACCGTAATTTGCCTGATCTTTGGCTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGCGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAgg                                                                                                                                                                    >  1:266852/1‑251 (MQ=255)                                                                                 aCCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAgg                                                                                                                                                                    <  1:15278/250‑1 (MQ=255)                                                                                  ccGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  <  1:306442/251‑1 (MQ=255)                                                                                  ccGTAATTTCCCTGATCTTGGTTTTGTCGCGCCACTTTTATGCCGAGGCGTTCAAAAAAGGTGGTTTGCACAAACCCGGAGAAATCCACGCCACTTCGAATTTCTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  <  1:86079/251‑1 (MQ=255)                                                                                  ccGTAATTGGCCTGATCTTTGGTTTGGCGCGGAAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAACCGGGAGTAACCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  <  1:452324/251‑1 (MQ=255)                                                                                          tGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATc                                                                                                                                                                                                                 <  1:713587/196‑1 (MQ=255)                                                                                            ccTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAAcaacaa                                                                                                                                                        <  1:841498/251‑1 (MQ=255)                                                                                                aTCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATa                                                                                                                                                     <  1:762954/250‑1 (MQ=255)                                                                                                aTCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATc                                                                                                                                                                                                                 <  1:484333/190‑1 (MQ=255)                                                                                                aTCTTTGGTTTGGCGGGGCAATTTTATGCCGAAGCGATGGAAAAAGGTGGTTGGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATa                                                                                                                                                     <  1:183899/250‑1 (MQ=255)                                                                                                 tCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGTGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAg                                                                                                                                                   <  1:451312/251‑1 (MQ=255)                                                                                                 tCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATc                                                                                                                                                                                                                 >  1:642732/1‑189 (MQ=255)                                                                                                 tCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAAc                                                                                                                                                                                     <  1:823586/217‑1 (MQ=255)                                                                                                 tCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAg                                                                                                                                                   >  1:827506/1‑251 (MQ=255)                                                                                                      ggTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTagag                                                                                                                                              >  1:549573/1‑251 (MQ=255)                                                                                                       gTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTGTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTagag                                                                                                                                              <  1:427395/250‑1 (MQ=255)                                                                                                                             cGAAGCGATCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAat                                                                                                                       <  1:628228/251‑1 (MQ=255)                                                                                                                                 gCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAatcat                                                                                                                    >  1:131928/1‑250 (MQ=255)                                                                                                                                    aTCGAAAAAGGTGGTTTGCACGAACCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCa                                                                                                                 <  1:44487/250‑1 (MQ=255)                                                                                                                                              gtggtTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAg                                                                                                       <  1:901402/250‑1 (MQ=255)                                                                                                                                                     gCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATa                                                                                                                                                     >  1:854695/1‑197 (MQ=255)                                                                                                                                                     gCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAg                                                                                                                                                   >  1:876613/1‑199 (MQ=255)                                                                                                                                                          aacccGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGt                                                                                                                                                                                               <  1:307862/147‑1 (MQ=255)                                                                                                                                                          aaaCCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  >  1:689892/1‑179 (MQ=255)                                                                                                                                                                gAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGc                                                                                                      <  1:152904/233‑1 (MQ=255)                                                                                                                                                                     acccACGCCGCTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  <  1:590895/166‑1 (MQ=255)                                                                                                                                                                     acccACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGaacaac                                                                                                                                                          <  1:304838/174‑1 (MQ=255)                                                                                                                                                                     aTCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  >  1:161391/1‑168 (MQ=255)                                                                                                                                                                     aTCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCAt                                                                                                                           >  1:756638/1‑207 (MQ=255)                                                                                                                                                                       ccACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAatca                                                                                                                     <  1:714717/211‑1 (MQ=255)                                                                                                                                                                                  cGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGc                                                                  <  1:59104/251‑1 (MQ=255)                                                                                                                                                                                              ccgATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATc                                                                                                                                                                                                                                   <  1:741731/78‑1 (MQ=255)                                                                                                                                                                                                 atacgataAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATa                                                                                                                                                     <  1:442013/153‑1 (MQ=255)                                                                                                                                                                                                   acgataAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGAc                                                                                                                                                                  >  1:549475/1‑138 (MQ=255)                                                                                                                                                                                                    cgataAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTag                                                                                                                                                >  1:699142/1‑155 (MQ=255)                                                                                                                                                                                                    cgataAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGcc                                                                              <  1:363228/221‑1 (MQ=255)                                                                                                                                                                                                                      cATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAg                                                                                                                                                                                                                                              >  1:312859/1‑43 (MQ=255)                                                                                                                                                                                                                      cATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGAt                                                                                                                                                                                                        >  1:547371/1‑81 (MQ=255)                                                                                                                                                                                                                        ttCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGaac                                                                                                                                                             <  1:89280/122‑1 (MQ=255)                                                                                                                                                                                                                         tCGTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAAccc                                           >  1:11823/1‑235 (MQ=255)                                                                                                                                                                                                                           gTATTGCTGTTCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGt                          >  1:366586/1‑250 (MQ=255)                                                                                                                                                                                                                                     tCGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGaa               >  1:653431/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGCTTAAAAGCGCAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGAac              <  1:571828/251‑1 (MQ=255)                                                                                                                                                                                                                                                cgcAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGAACACCACCacac    <  1:596553/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cAATCGCCATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGAACACCACCACacga  <  1:352754/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CATGATAGCCATGAGGACCGCGACCGGTTTTAAAGAACACCAAATCACC‑GGTTTGAATATCGCCTTTTTCAATATACTGACCGTAATTTGCCTGATCTTTGGTTTGTCGCGGCAATTTTATGCCGAAGCGATCGAAAAAGGTGGTTTGCACGAAACCGGAGCAATCCACGCCACTGCGACTTTGTCCGCCGATACGATAAGGCGTTCCCGCCCATTCGTATTGCTGTTCGCTTAAAAGCGCAATCGCTATAATAGGATCGTTTATCTGCCCTTTGTAATTAATCCGCGAGGATTTTGACGGTCCTCCGGAACAGGCGGCTAACGCCAGGACAGAACAACAAATAAGTAGAGATTTAAAAGAAATACGCATAAATCATCCATAACAAAAAGCTACCGACAATCGCTTGCGGTAGCCAATTTATTAAGCTTTCGGTTTGACCTTTTCAACCCGGTTGCGCAATTTTTGTCCCGGTTTGAACACCACCACACGA  >  1/1103646‑1104136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑