Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,103,318	A→C	G284G (GGA→GGC)	unknown →	tRNA‑(cytosine32)‑2‑thiocytidine synthetase TtcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,103,318	0	A	C	100.0%	210.8 / NA	75	G284G (GGA→GGC)	unknown	tRNA‑(cytosine32)‑2‑thiocytidine synthetase TtcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (44/31);  total (44/31)

GCAAAGAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGAGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGCCTTACGCC  >  1/1103071‑1103561                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gCAAAGAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTg                                                                                                                                                                                                                                                  >  1:413334/1‑251 (MQ=255)    aaGAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGaa                                                                                                                                                                                                                                               <  1:132359/251‑1 (MQ=255)      gAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAgg                                                                                                                                                                                                                                             >  1:107350/1‑251 (MQ=255)      gAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAgg                                                                                                                                                                                                                                             >  1:892981/1‑251 (MQ=255)                  gAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCAtt                                                                                                                                                                                                                                 >  1:812469/1‑251 (MQ=255)                                cgcgAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACa                                                                                                                                                                                                                   >  1:467802/1‑251 (MQ=255)                                      ggCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACg                                                                                                                                                                                                              >  1:734934/1‑250 (MQ=255)                                              cGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGc                                                                                                                                                                                                       >  1:275301/1‑249 (MQ=255)                                                   attCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGaa                                                                                                                                                                                                                                               >  1:91825/1‑204 (MQ=255)                                                              tCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAgg                                                                                                                                                                                                                                             <  1:163298/195‑1 (MQ=255)                                                              tCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGAt                                                                                                                                                                                      >  1:257035/1‑250 (MQ=255)                                                               cTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCa                                                                                                                                                                                    >  1:884898/1‑251 (MQ=255)                                                                      gTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGaa                                                                                                                                                                                                                                               >  1:669452/1‑185 (MQ=255)                                                                                        aaCGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATg                                                                                                                                                           <  1:177365/251‑1 (MQ=255)                                                                                            tCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATc                                                                                                                                                        <  1:117682/250‑1 (MQ=255)                                                                                                 tGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCttt                                                                                                                                                  >  1:35833/1‑251 (MQ=255)                                                                                                  ggTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTa                                                                                                                                                 >  1:747632/1‑251 (MQ=255)                                                                                                  ggTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTa                                                                                                                                                 >  1:329792/1‑251 (MQ=255)                                                                                                     aaaaGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              <  1:878577/251‑1 (MQ=255)                                                                                                      aaaGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGa                                                                                                                                             <  1:687444/251‑1 (MQ=255)                                                                                                      aaaGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGa                                                                                                                                             <  1:659600/251‑1 (MQ=255)                                                                                                       aaGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATc                                                                                                                                                                                     <  1:190425/210‑1 (MQ=255)                                                                                                            aTGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTtgatg                                                                                                                                                                                              <  1:184483/196‑1 (MQ=255)                                                                                                                tGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAg                                                                                                                                   <  1:786388/251‑1 (MQ=255)                                                                                                                 gAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGa                                                                                                                                             <  1:773374/240‑1 (MQ=255)                                                                                                                   aTACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTtgatga                                                                                                                                                                                             >  1:432607/1‑190 (MQ=255)                                                                                                                   aTACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGaa                                                                                                                                >  1:615117/1‑251 (MQ=255)                                                                                                                                ccAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGaaa                                                                                                                                                             >  1:271790/1‑209 (MQ=255)                                                                                                                                    tatCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTtgatg                                                                                                                                                                                                                            >  1:16652/1‑142 (MQ=255)                                                                                                                                           gACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCAc                                                                                                         <  1:657509/250‑1 (MQ=255)                                                                                                                                               cTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              >  1:563890/1‑209 (MQ=255)                                                                                                                                               cTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTa                                                                                                    >  1:854840/1‑251 (MQ=255)                                                                                                                                               cTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATCTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTa                                                                                                    >  1:406051/1‑251 (MQ=255)                                                                                                                                                   gAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCGCCTGTGTGAACCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACgg                                                                                                 <  1:560085/250‑1 (MQ=255)                                                                                                                                                         aTGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAAt                                                                                                                                                            <  1:334192/185‑1 (MQ=255)                                                                                                                                                           gTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              >  1:681170/1‑197 (MQ=255)                                                                                                                                                           gTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAg                                                                                                                                   <  1:400600/208‑1 (MQ=255)                                                                                                                                                                gcgcCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGcaaacaa                                                                                   >  1:364152/1‑251 (MQ=255)                                                                                                                                                                 cgcCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGcaaac                                                                                     <  1:299781/248‑1 (MQ=255)                                                                                                                                                                     aTGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGa                                                                                                                                             >  1:651275/1‑188 (MQ=255)                                                                                                                                                                          aaaCATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCgg                                                                         >  1:295978/1‑251 (MQ=255)                                                                                                                                                                             cATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGttt                                                                      >  1:740114/1‑251 (MQ=255)                                                                                                                                                                                    ttGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGc                                                                <  1:301267/250‑1 (MQ=255)                                                                                                                                                                                     tGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACAt                                                                                                                           <  1:544425/190‑1 (MQ=255)                                                                                                                                                                                     tGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCaa                                                              >  1:265336/1‑251 (MQ=255)                                                                                                                                                                                      gTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTtgatga                                                                                                                                                                                                                           >  1:194939/1‑93 (MQ=255)                                                                                                                                                                                      gTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGaaa                                                                                                                                                             >  1:149106/1‑155 (MQ=255)                                                                                                                                                                                      gTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              <  1:98611/170‑1 (MQ=255)                                                                                                                                                                                      gTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              <  1:458905/170‑1 (MQ=255)                                                                                                                                                                                      gTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCaa                                                              >  1:793566/1‑250 (MQ=255)                                                                                                                                                                                          cACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGAtt                                                         >  1:407007/1‑251 (MQ=255)                                                                                                                                                                                            ccTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAAc                                                                                                                             <  1:168576/181‑1 (MQ=255)                                                                                                                                                                                               gtgtgATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATc                                                                                                                                                                                     >  1:189869/1‑122 (MQ=255)                                                                                                                                                                                                    aTCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGc                                                                                                                                                                                                             >  1:549806/1‑93 (MQ=255)                                                                                                                                                                                                    aTCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAgg                                                                                                                                  <  1:519109/168‑1 (MQ=255)                                                                                                                                                                                                    aTCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAgg                                                                                                                                  <  1:890415/168‑1 (MQ=255)                                                                                                                                                                                                       cAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTg                                            <  1:646241/251‑1 (MQ=255)                                                                                                                                                                                                            cTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              >  1:259828/1‑148 (MQ=255)                                                                                                                                                                                                                       cAAAGGCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACgg                                                                                                 >  1:701502/1‑182 (MQ=255)                                                                                                                                                                                                                           ggCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCtt                        >  1:215178/1‑251 (MQ=255)                                                                                                                                                                                                                            gCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTtg                       >  1:732723/1‑251 (MQ=255)                                                                                                                                                                                                                            gCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTtg                       >  1:482416/1‑251 (MQ=255)                                                                                                                                                                                                                            gCATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTtg                       >  1:788186/1‑251 (MQ=255)                                                                                                                                                                                                                             cATTAAACACGGTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTa                                          <  1:682764/231‑1 (MQ=255)                                                                                                                                                                                                                                       ggTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAatat            >  1:452620/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAgg                                                                                                                                              <  1:789017/120‑1 (MQ=255)                                                                                                                                                                                                                                        gTCAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATg           >  1:374731/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGcc         <  1:303417/251‑1 (MQ=255)                                                                                                                                                                                                                                          cAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGcc         <  1:759230/251‑1 (MQ=255)                                                                                                                                                                                                                                          cAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGc          >  1:28187/1‑250 (MQ=255)                                                                                                                                                                                                                                          cAATTGATTGAAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGc          >  1:174137/1‑250 (MQ=255)                                                                                                                                                                                                                                               gattgaAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATTACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTAtg                                        <  1:426430/215‑1 (MQ=255)                                                                                                                                                                                                                                               gattgaAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTAtg                                        <  1:143655/215‑1 (MQ=255)                                                                                                                                                                                                                                               gattgaAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGCCTTACg    >  1:620823/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttgaAGGCGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGCCTTACGcc  <  1:205541/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GCAAAGAAAAAGACATTGAAAAATATGCCGTCGCGAAGGCTTTTCCGATTATTCCTTGCAATCTGTGCGGTTCGCAACCGAATCTGCAACGTCAAGTGGTAAAAGAAATGCTGAATACCTGGGATCGCCAATATCCGGGACGCTTGGAAACCATGTTCAGCGCCATGCAAAACATTACGTTGTCTCACCTGTGTGATCCAAAACTTTTTGATTTCAAAGGCATTAAACACGGTCAATTGATTGAAGGAGTGGAAGGCGATACGGCATTTGATGAAGAAAACATCACGCCGATGCAATTTGATGATGAAGATCAGGCGGATTTCAGCGAGCATGAAATGATCGCCTTTAAGGAAGTGCGGTAGGAAAACATCGGAAAAAATGACCGCACTTTTACGGCGATATGCAAACAAAAAATCGCGGTTTTTACGCAACGATTTTTCTAGAAATTGTATGTGATTAAATTCGTCTTGCCTGCCAATATGCCTTACGCC  >  1/1103071‑1103561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑