Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,085,324	T→C	R140R (CGT→CGC)	unknown →	HlyD‑like membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,085,324	0	T	C	100.0%	201.2 / NA	74	R140R (CGT→CGC)	unknown	HlyD‑like membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/51);  total (23/51)

ATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGTCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTATATCAATGT  >  1/1085079‑1085569                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aTTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAg                                                                                                                                                                                                                                                   >  1:448065/1‑250 (MQ=255)        tGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTtat                                                                                                                                                                                                                                           <  1:417264/251‑1 (MQ=255)        tGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTtat                                                                                                                                                                                                                                           <  1:843468/251‑1 (MQ=255)             gaaagTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAgg                                                                                                                                                                                                                                       <  1:522782/246‑1 (MQ=255)             gaaagTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAgg                                                                                                                                                                                                                                       <  1:728341/246‑1 (MQ=255)             gaaagTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAgg                                                                                                                                                                                                                                       <  1:602460/246‑1 (MQ=255)              aaagTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAgg                                                                                                                                                                                                                                       >  1:337253/4‑249 (MQ=255)                   aaTGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACt                                                                                                                                                                                                                                <  1:525754/251‑1 (MQ=255)                    aTGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTc                                                                                                                                                                                                                               <  1:308311/251‑1 (MQ=255)                         gTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTg                                                                                                                                                                                                                           >  1:31622/1‑250 (MQ=255)                         gTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTg                                                                                                                                                                                                                           >  1:592276/1‑250 (MQ=255)                         gTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTg                                                                                                                                                                                                                           >  1:543983/1‑250 (MQ=255)                                 cAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCt                                                                                                                                                                                                                            <  1:396374/241‑1 (MQ=255)                                 cAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAac                                                                                                                                                                                                                   <  1:590856/250‑1 (MQ=255)                                 cAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAAAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTAAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGCATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAac                                                                                                                                                                                                                   <  1:769190/250‑1 (MQ=255)                                   aTCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAac                                                                                                                                                                                                                   <  1:285086/248‑1 (MQ=255)                                                   gCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCaaa                                                                                                                                                                                                 <  1:63245/250‑1 (MQ=255)                                                    cTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGc                                                                                                                                                                                               <  1:194698/251‑1 (MQ=255)                                                       tcttcCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAacac                                                                                                                                                                                                                 <  1:654272/230‑1 (MQ=255)                                                                         gcgcAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCg                                                                                                                                                                           >  1:707010/1‑250 (MQ=255)                                                                            cAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGcac                                                                                                                                                                        <  1:746943/250‑1 (MQ=255)                                                                                   ccgccgCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAg                                                                                                                                                                                                                                        <  1:541347/179‑1 (MQ=255)                                                                                         cacaGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAg                                                                                                                                                          <  1:868928/251‑1 (MQ=255)                                                                                         cacaGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAg                                                                                                                                                          >  1:876226/1‑251 (MQ=255)                                                                                               cacaAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGaa                                                                                                                                                    <  1:822345/251‑1 (MQ=255)                                                                                               cacaAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGaa                                                                                                                                                    <  1:742751/251‑1 (MQ=255)                                                                                                 caAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACa                                                                                                                                                  <  1:137852/251‑1 (MQ=255)                                                                                                 caAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACa                                                                                                                                                  <  1:691411/251‑1 (MQ=255)                                                                                                 caAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAAGTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACa                                                                                                                                                  <  1:428504/251‑1 (MQ=255)                                                                                                       aaaCGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAgcg                                                                                                                                              <  1:651893/249‑1 (MQ=255)                                                                                                                   aaaTCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATAc                                                                                                                                <  1:824631/250‑1 (MQ=255)                                                                                                                     aTCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCg                                                                                                                              >  1:594539/1‑251 (MQ=255)                                                                                                                      tCCGTTGCCCGCGACCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATGGAATAACGCACAAAAATGGCGCCTCGATATTCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGATCAAGCGCAATCGCAAAATACCGa                                                                                                                             <  1:306796/251‑1 (MQ=255)                                                                                                                        cGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGata                                                                                                                           <  1:368006/251‑1 (MQ=255)                                                                                                                            gCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATg                                                                                                                         >  1:463694/1‑249 (MQ=255)                                                                                                                            gCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATg                                                                                                                         >  1:306/1‑249 (MQ=255)                                                                                                                                 cgcCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGAtt                                                                                                                   <  1:716163/250‑1 (MQ=255)                                                                                                                                   cccTATCAGAAATTGATGCCCGCCAACAACAAGTCAATGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAgcgc                                                                                                                                                            <  1:448640/207‑1 (MQ=255)                                                                                                                                   cccTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGaa                                                                                                                                                    >  1:53139/1‑215 (MQ=255)                                                                                                                                       aTCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGc                                                                                                                                                                                                                          >  1:151796/1‑141 (MQ=255)                                                                                                                                       aTCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGaa                                                                                                                                                    <  1:268645/211‑1 (MQ=255)                                                                                                                                       aTCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATAc                                                                                                                                >  1:647545/1‑231 (MQ=255)                                                                                                                                       aTCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAgcg                                                                                                             >  1:324967/1‑250 (MQ=255)                                                                                                                                       aTCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAA‑‑‑GCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACa                                                                                                                                                  <  1:251589/210‑1 (MQ=255)                                                                                                                                                             caacaaGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGc                                                                                                                                                                                                                                                  <  1:450581/95‑1 (MQ=255)                                                                                                                                                             caacaaGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAata                                                                                      >  1:501025/1‑251 (MQ=255)                                                                                                                                                                caaGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATAc                                                                                                                                <  1:861902/206‑1 (MQ=255)                                                                                                                                                                       aCGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAAc                                                                                                                                                   >  1:885489/1‑180 (MQ=255)                                                                                                                                                                        cGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCgg                                                                           <  1:770820/251‑1 (MQ=255)                                                                                                                                                                               aaaTTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAgg                                                                    >  1:280553/1‑251 (MQ=255)                                                                                                                                                                                aaTTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGc                                                                   <  1:203309/251‑1 (MQ=255)                                                                                                                                                                                     gAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGaaa                                                                                                       >  1:132057/1‑210 (MQ=255)                                                                                                                                                                                       aTTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCg                                                                                                                                                                                                                                     <  1:462090/82‑1 (MQ=255)                                                                                                                                                                                          gAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGc                                                                                                                        <  1:844449/188‑1 (MQ=255)                                                                                                                                                                                          gAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCaa                                                                                  <  1:159526/226‑1 (MQ=255)                                                                                                                                                                                                  cacaAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGa                                                                                                                             <  1:29834/175‑1 (MQ=255)                                                                                                                                                                                                  cacaAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATACGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGt                                                 >  1:688473/1‑251 (MQ=255)                                                                                                                                                                                                    caAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAgg                                                                                                                                                                     >  1:672522/1‑133 (MQ=255)                                                                                                                                                                                                                 tCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTgg                                                                                          >  1:396229/1‑195 (MQ=255)                                                                                                                                                                                                                 tCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGt                                  >  1:885996/1‑251 (MQ=255)                                                                                                                                                                                                                 tCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGt                                  <  1:450635/251‑1 (MQ=255)                                                                                                                                                                                                                    aTAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGttt                                <  1:540487/250‑1 (MQ=255)                                                                                                                                                                                                                    aTAATCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGttt                                <  1:245572/250‑1 (MQ=255)                                                                                                                                                                                                                        tCTGATTTCAAGCAGCGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCg                           <  1:726322/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATAcc             <  1:705704/250‑1 (MQ=255)                                                                                                                                                                                                                                       cGAATTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATAcc             <  1:457269/250‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTaa                                                                                                                 <  1:12997/148‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATAcc             <  1:890138/248‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCtat          >  1:422766/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTatat        <  1:313329/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttAGAACACCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTatat        <  1:173686/251‑1 (MQ=255)                                                                                                                                                                                                                                             tgcctacCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTATATc       <  1:864002/245‑1 (MQ=255)                                                                                                                                                                                                                                                 cacCGCCAAGCCGGTTATAAGGCGTCCCTCGCTGCCGTGAACACCACGGAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTATATCAATGt  <  1:679723/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cacCGCCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTATATCAATGt  <  1:742769/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACACCGTCAAGCCGCTTATAAGGCGTCACTCGCTGCCGTGAACACCACGCAAGCCGCCAAAGCCGAAGCACAAGCCGCCGTCGCACAGGCGCAAGCGCAGTTAGAACAAGCGCAATCGCAAAATACCGATATGCTGATTAAAGCGCCGAAAGACGGACGGGTGGAATATCAAATTGCGGAAGTAGGCAATGTTCTCGGTGCCGGTGGCAAAGTGGTCAGTTTGCTCGATCCGACGGATACCTATATCAATGT  >  1/1085079‑1085569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑