Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,076,984	C→T	E253E (GAG→GAA)	unknown ←	DNA topoisomerase I (EC 5.99.1.2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,076,984	0	C	T	100.0%	179.9 / NA	66	E253E (GAG→GAA)	unknown	DNA topoisomerase I (EC 5.99.1.2)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/43);  total (23/43)

TTGGTGGAGTCGGTACGCATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGCTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGCCTACCAC  >  1/1076748‑1077205                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              ttGGTGGAGTCGGTACGCATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTAcc                                                                                                                                                                                                                 <  1:261776/251‑1 (MQ=255)   ggtggAGTCGGTACGCATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCtt                                                                                                                                                                                                               >  1:147243/1‑251 (MQ=255)          tCGGTACGCATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAAt                                                                                                                                                                                                          <  1:665236/249‑1 (MQ=255)                  cATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACAt                                                                                                                                                                                                <  1:321265/251‑1 (MQ=255)                  cATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGCTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACAt                                                                                                                                                                                                >  1:782778/1‑251 (MQ=255)                   atatAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATc                                                                                                                                                                                               <  1:871133/251‑1 (MQ=255)                   atatAAGGGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTGGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACcacacc                                                                                                                                                                                               <  1:871106/251‑3 (MQ=255)                     ataAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCa                                                                                                                                                                                             <  1:191140/251‑1 (MQ=255)                        aGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAg                                                                                                                                                                                           >  1:589139/1‑250 (MQ=255)                          tGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCg                                                                                                                                                                                         <  1:639982/250‑1 (MQ=255)                           gATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGaa                                                                                                                                                                                       >  1:121586/1‑251 (MQ=255)                            aTGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAAt                                                                                                                                                                                      >  1:218198/1‑251 (MQ=255)                            aTGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAAt                                                                                                                                                                                      >  1:872560/1‑251 (MQ=255)                              gTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAAttt                                                                                                                                                                                    >  1:701561/1‑251 (MQ=255)                                         cATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCttt                                                                                                                                                                          >  1:46953/1‑250 (MQ=255)                                          aTACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTAcc                                                                                                                                                                                                                 <  1:66148/210‑1 (MQ=255)                                           tACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAAt                                                                                                                                                                                                          <  1:847776/216‑1 (MQ=255)                                           tACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCgg                                                                                                                                                                       >  1:72561/1‑251 (MQ=255)                                            aCAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGgt                                                                                                                                                                      <  1:467270/251‑1 (MQ=255)                                                   ttGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTgg                                                                                                                                                               <  1:465439/251‑1 (MQ=255)                                                   ttGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTCTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTgg                                                                                                                                                               <  1:274609/251‑1 (MQ=255)                                                    tGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTT‑GTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTg                                                                                                                                                             <  1:697664/251‑1 (MQ=255)                                                      cgcCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTg                                                                                                                                                             <  1:203580/250‑1 (MQ=255)                                                      cgcCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTg                                                                                                                                                             <  1:891678/250‑1 (MQ=255)                                                      cgcCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGa                                                                                                                                                            >  1:199623/1‑251 (MQ=255)                                                          aaCATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCAc                                                                                                                                                        <  1:379448/251‑1 (MQ=255)                                                                atGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGAc                                                                                                                                                  <  1:467360/251‑1 (MQ=255)                                                                             cacCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTgg                                                                                                                                                               >  1:34581/1‑225 (MQ=255)                                                                             cacCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTCGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTcc                                                                                                                                     <  1:443897/251‑1 (MQ=255)                                                                                    gCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTg                                                                                                                                                             >  1:615935/1‑220 (MQ=255)                                                                                    gCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGc                                                                                                                                                          <  1:199912/223‑1 (MQ=255)                                                                                                           gttgtAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATc                                                                                                                                                                                               <  1:349738/163‑1 (MQ=255)                                                                                                           gttgtAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTAt                                                                                                                                                                  <  1:555332/192‑1 (MQ=255)                                                                                                           gttgtAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTc                                                                                                                                      <  1:600325/220‑1 (MQ=255)                                                                                                                      gAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGAt                                                                                            >  1:569755/1‑251 (MQ=255)                                                                                                                         gTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTa                                                                                          <  1:230624/250‑1 (MQ=255)                                                                                                                           aataaACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAAt                                                                                                                                                                                                          >  1:31179/1‑136 (MQ=255)                                                                                                                           aataaACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTaa                                                                                         >  1:867127/1‑249 (MQ=255)                                                                                                                           aataaACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACt                                                                                       <  1:182293/251‑1 (MQ=255)                                                                                                                                   gcgcTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACt                                                                                       >  1:277025/1‑243 (MQ=255)                                                                                                                                      ctctTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAAttt                                                                                                               >  1:768199/1‑216 (MQ=255)                                                                                                                                        ctTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGa                                                                          >  1:385479/1‑251 (MQ=255)                                                                                                                                                ggAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGcaca                                                                   >  1:196798/1‑250 (MQ=255)                                                                                                                                                   aCTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGAc                                                               <  1:725066/251‑1 (MQ=255)                                                                                                                                                    cTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGAcc                                                              <  1:721573/251‑1 (MQ=255)                                                                                                                                                        tCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCTAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGc                                                           <  1:353418/250‑1 (MQ=255)                                                                                                                                                        tCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTCTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGc                                                           <  1:435811/250‑1 (MQ=255)                                                                                                                                                           gCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGAc                                                               <  1:721608/243‑1 (MQ=255)                                                                                                                                                                gTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAAc                                                   <  1:197025/250‑1 (MQ=255)                                                                                                                                                                gTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAAc                                                   <  1:837138/250‑1 (MQ=255)                                                                                                                                                                gTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAAc                                                   <  1:214147/250‑1 (MQ=255)                                                                                                                                                                gTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAAc                                                   <  1:343237/250‑1 (MQ=255)                                                                                                                                                                               aTCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACtttttt                                   <  1:327439/251‑1 (MQ=255)                                                                                                                                                                                  acaTAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTgg                                                                                                                                                               >  1:406832/1‑124 (MQ=255)                                                                                                                                                                                   caTAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAat                                                                                                                                          >  1:646420/1‑144 (MQ=255)                                                                                                                                                                                        tCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGt                            <  1:672932/249‑1 (MQ=255)                                                                                                                                                                                                 cTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGAc                 <  1:897062/251‑1 (MQ=255)                                                                                                                                                                                                   tAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTg                     >  1:611728/1‑245 (MQ=255)                                                                                                                                                                                                    aaaaaaTCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCAt              <  1:112528/251‑1 (MQ=255)                                                                                                                                                                                                       aaaTCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATaaa           <  1:219532/251‑1 (MQ=255)                                                                                                                                                                                                         aTCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGc         <  1:411294/251‑1 (MQ=255)                                                                                                                                                                                                         aTCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACATCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGc         <  1:184291/251‑1 (MQ=255)                                                                                                                                                                                                           ccACCGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCGCTTTTTTcc                                 >  1:335965/1‑225 (MQ=255)                                                                                                                                                                                                               cGCACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGCCTACCa   <  1:60737/251‑1 (MQ=255)                                                                                                                                                                                                                 cACTTTGCGCCTGCTCGGCATTTTTCGGTTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGCCTACCAc  <  1:766059/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCATTGTTCGGTTCGAATTTCTTACCTTTAAATTGAACCTCATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAAttt                                                                                                               <  1:810729/124‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              TTGGTGGAGTCGGTACGCATATAAGTGATGTAACCGGCTTCATACAAACGTTGCGCCAACATCATGGTTTTTTTCACACCGAAGCCTAAACGGGTGCTAGCGGTTTGTTGTAAGGTTGAGGTAATAAACGGCGCTCTTGGGCGGGAACTGGTCGGCTTGGTTTCCAAATCGGAAATCACATAGTCGGATTTACTTAAAAAATCCACCGCACTTTGCGCCTGCTCGGCATTTTTCGGCTCGAATTTCTTACCTTTAAATTGAACCACATCCAAGCGAATTTCATCGCCTTTCGGTGTATTGGTGAGCACGGAGACTTCCCAATATTCCTGTGGCACGAAAGCCTTAATTTCACGTTCACGCTCAACGATTAACTTCACCGCCACCGATTGCACACGACCGGCAGATAAACCGCGCGCCACTTTTTTCCAAAGTAACGGTGAGACCATAAAGCCTACCAC  >  1/1076748‑1077205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑