BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,075,101	T→C	intergenic (+50/+35)	unknown → / ← unknown	ABC‑type Co2+ transport system, periplasmic component/DNA topoisomerase I (EC 5.99.1.2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,075,101	0	T	C	100.0%	120.5 / NA	44	intergenic (+50/+35)	unknown/unknown	ABC‑type Co2+ transport system, periplasmic component/DNA topoisomerase I (EC 5.99.1.2)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/19); total (25/19)

TTCTTACCGTTAGTTGAAGGTTTATGGAAAGTAAAAGTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAATCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGATATAACGCCAATTCCG  >  1/1074887‑1075341

ttCTTACCGTTAGTTGAAGGTTTATGGAAAGTAAAAGTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGtttt                                                                                                                                                                                                              >  1:145253/1‑251 (MQ=255)

ttCTTACCGTTAGTTGAAGGTTTATGGAAAGTAAAAGTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGtttt                                                                                                                                                                                                              >  1:364580/1‑251 (MQ=255)

                              gTAAAAGTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATcaa                                                                                                                                                                                >  1:656070/1‑251 (MQ=255)

                                    gTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAAt                                                                                                                                                                          >  1:622493/1‑251 (MQ=255)

                                    gTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAAt                                                                                                                                                                          >  1:776450/1‑251 (MQ=255)

                                         acacAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATc                                                                                                                                                                                                                    <  1:862467/204‑1 (MQ=255)

                                                aTTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCAc                                                                                                                                                                      <  1:589127/243‑1 (MQ=255)

                                                   ccGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTcccc                                                                                                                                                                                            <  1:97405/218‑1 (MQ=255)

                                                             aCCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGc                                                                                                                                                  >  1:830532/1‑250 (MQ=255)

                                                              cccTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATc                                                                                                                                                                                                                    <  1:556418/183‑1 (MQ=255)

                                                              cccTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTAt                                                                                                                                                                   <  1:3060/232‑1 (MQ=255)

                                                                 tAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTc                                                                                                                                             <  1:358510/251‑1 (MQ=255)

                                                                     gtgtgtCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCtttt                                                                                                                                         >  1:98905/1‑251 (MQ=255)

                                                                       gtgtCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTaag                                                                                                                                                                                                                                     <  1:206603/157‑1 (MQ=255)

                                                                                                aCGCTGGTATTGCCGGTGGGCGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCAGTTAAAAATTTAATTGTTTTTTTAACAGCACTTTTTAAGAAGCATTAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCtt                                                                                                              >  1:600154/1‑251 (MQ=255)

                                                                                                     ggTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGa                                                                                                         >  1:57278/1‑251 (MQ=255)

                                                                                                       tATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGAc                                                                                                        <  1:280151/250‑1 (MQ=255)

                                                                                                            ccGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAg                                                                                                   <  1:352390/250‑1 (MQ=255)

                                                                                                            ccGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAg                                                                                                   <  1:534482/250‑1 (MQ=255)

                                                                                                                      aaaaaCGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAatgatg                                                                                          <  1:635176/249‑1 (MQ=255)

                                                                                                                               cgGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGc                                                                                <  1:20145/250‑1 (MQ=255)

                                                                                                                                 gAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTAc                                                                                                           >  1:230802/1‑221 (MQ=255)

                                                                                                                                   atatAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCAtttt                                                                                                                                                            <  1:608443/170‑1 (MQ=255)

                                                                                                                                   atatAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATgg                                                                                         >  1:622560/1‑237 (MQ=255)

                                                                                                                                      taAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGa                                                                        <  1:279874/251‑1 (MQ=255)

                                                                                                                                          ccTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAatgatg                                                                                          >  1:224020/1‑229 (MQ=255)

                                                                                                                                              aCGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTtg                                                                <  1:756673/251‑1 (MQ=255)

                                                                                                                                                  aCATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTgtggt                                                            <  1:730246/251‑1 (MQ=255)

                                                                                                                                                   cATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTGCGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTgtggtg                                                           >  1:893960/1‑251 (MQ=255)

                                                                                                                                                     tCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGt                                                                                                                     >  1:291339/1‑191 (MQ=255)

                                                                                                                                                          accaTCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCAc                                                                                                                                                                                                                                             >  1:724727/1‑66 (MQ=255)

                                                                                                                                                           ccaTCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGGAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGc                                                    >  1:88332/1‑250 (MQ=255)

                                                                                                                                                           ccaTCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGcc                                                   <  1:418286/251‑1 (MQ=255)

                                                                                                                                                           ccaTCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGc                                                    >  1:18657/1‑250 (MQ=255)

                                                                                                                                                                        cTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATAtt                                                                                                                          >  1:234452/1‑167 (MQ=255)

                                                                                                                                                                                aaTAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGcc                                                   >  1:515872/1‑230 (MQ=255)

                                                                                                                                                                                 aTAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAgg                                                                                                                                   <  1:318625/149‑1 (MQ=255)

                                                                                                                                                                                 aTAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCa                             >  1:195885/1‑251 (MQ=255)

                                                                                                                                                                                             gTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCt                                                                              >  1:188614/1‑190 (MQ=255)

                                                                                                                                                                                             gTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGat                 >  1:474304/1‑251 (MQ=255)

                                                                                                                                                                                             gTTAAAAATTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGat                 >  1:39495/1‑251 (MQ=255)

                                                                                                                                                                                                    aTTTAATTGTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGATATAACGc          <  1:663604/251‑1 (MQ=255)

                                                                                                                                                                                                            gTTTTTTTAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGATATAACGCCAATTCCg  >  1:378417/1‑251 (MQ=255)

                                                                                                                                                                                                             tttttttAACCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCAc                                                                                   >  1:250794/1‑169 (MQ=255)

TTCTTACCGTTAGTTGAAGGTTTATGGAAAGTAAAAGTTATACACAAGATTCCGTTTGGTGACCCTAAAGTGTGTCAACATTCGGCAAATTACGCGACGCTGGTATTGCCGGTGGGAGAAAAACGCGCGGAATATAAACCTCACGAACATCACCACCATCACTAATTTCTTTTATCAATAAAAAGTGCGGTTAAAAATTTAATTGTTTTTTTAATCGCACTTTTTAAGAAGCATGAACGAAAATCTGTTTTATTTCTTTCGCTCTACCCACTTCCCATCAACAAAATCCACTATCCATTTTGTCGCCTTGCCATTCTTTTCGGAGGTGACATATTGACGTTTTTCTTTACGACTAAAGCGAATGATGGCTTCACTGCCTTCCGGATCCGTTTGTGGTGCGTCAGCCAGATAGCTTAATTTTTCCGGCAGCCGCTCACGATATAACGCCAATTCCG  >  1/1074887‑1075341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`