Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,067,531	C→A	intergenic (+6/‑242)	unknown → / → unknown	Exodeoxyribonuclease I (EC 3.1.11.1)/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,067,531	0	C	A	100.0%	161.3 / NA	64	intergenic (+6/‑242)	unknown/unknown	Exodeoxyribonuclease I (EC 3.1.11.1)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/36);  total (28/36)

AATTGCTGTTCCATTATCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGCTCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGA  >  1/1067311‑1067764                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            aaTTGCTGTTCCATTATCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAAtt                                                                                                                                                                                                              <  1:46209/250‑1 (MQ=255)  aTTGCTGTTCCATTATCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTg                                                                                                                                                                                                            <  1:8479/251‑1 (MQ=255)             aTTATCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGAtt                                                                                                                                                                                                 <  1:420961/251‑1 (MQ=255)                 tCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGt                                                                                                                                                                                             <  1:563593/251‑1 (MQ=255)                  cGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                            <  1:359111/251‑1 (MQ=255)                          aCATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTGTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                      <  1:435839/249‑1 (MQ=255)                           cATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTAc                                                                                                                                                                                        >  1:765983/1‑246 (MQ=255)                             tttCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTa                                                                                                                                                                                                >  1:408206/1‑236 (MQ=255)                             tttCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTAt                                                                                                                                                                                  >  1:196967/1‑250 (MQ=255)                             tttCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTAt                                                                                                                                                                                  >  1:733597/1‑250 (MQ=255)                              ttCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCa                                                                                                                                                                                <  1:172015/251‑1 (MQ=255)                                cTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAACATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAAt                                                                                                                                                                              >  1:808625/1‑251 (MQ=255)                                cTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAAt                                                                                                                                                                              <  1:422271/251‑1 (MQ=255)                                       aCATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTa                                                                                                                                                                       >  1:648728/1‑251 (MQ=255)                                       aCATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑C‑CCCCAAAACCTGGACAACTAATTTTAGGGGCAGATTATGTCTTACTCCTATCAATTTCGTTaa                                                                                                                                                                      >  1:366385/1‑251 (MQ=255)                                                      gAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAAc                                                                                                                                                         <  1:383293/250‑1 (MQ=255)                                                           aaTTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGt                                                                                                                                                     >  1:293506/1‑251 (MQ=255)                                                                                ccGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATc                                                                                                                               <  1:759570/250‑1 (MQ=255)                                                                                       aaaTTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAAc                                                                                                                                                         >  1:115329/1‑217 (MQ=255)                                                                                               tGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAAt                                                                                                               <  1:511985/251‑1 (MQ=255)                                                                                                                ttGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTg                                                                                                                                                                                                            <  1:276561/141‑1 (MQ=255)                                                                                                                         gTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTc                                                                                                                                                                                            <  1:607703/148‑1 (MQ=255)                                                                                                                         gTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGt                                                                                      >  1:709458/1‑250 (MQ=255)                                                                                                                          tCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTc                                                                                     <  1:839570/250‑1 (MQ=255)                                                                                                                           cTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCAt                                                                                   <  1:882102/251‑1 (MQ=255)                                                                                                                                 gAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGt                                                                                      <  1:41881/242‑1 (MQ=255)                                                                                                                                      aGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTActc                                                                                                                                                                                      >  1:899862/1‑141 (MQ=255)                                                                                                                                           aaCAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCt                                                                    <  1:878696/250‑1 (MQ=255)                                                                                                                                            aCAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCttt                                                                  >  1:513464/1‑251 (MQ=255)                                                                                                                                             cAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATG‑ATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCg                                                                >  1:571705/1‑251 (MQ=255)                                                                                                                                               atatTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGAtt                                                                                                                                                                                                 <  1:51085/121‑1 (MQ=255)                                                                                                                                               atatTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGt                                                               >  1:302902/1‑251 (MQ=255)                                                                                                                                                            gaagaaAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTc                                                                                                                                                    >  1:414361/1‑153 (MQ=255)                                                                                                                                                                   aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTgg                                                                            <  1:192390/218‑1 (MQ=255)                                                                                                                                                                   aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                              <  1:122950/248‑1 (MQ=255)                                                                                                                                                                   aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGt                                           >  1:775909/1‑251 (MQ=255)                                                                                                                                                                               tGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATc                                     <  1:270491/245‑1 (MQ=255)                                                                                                                                                                                  aaaaGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            <  1:27890/251‑1 (MQ=255)                                                                                                                                                                                     aGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAATTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            >  1:587128/1‑248 (MQ=255)                                                                                                                                                                                     aGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATaaa                         >  1:524451/1‑251 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCGGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:437133/1‑250 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCGGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:429091/1‑250 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                                <  1:150816/226‑1 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCt                                 <  1:583539/241‑1 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:558549/1‑250 (MQ=255)                                                                                                                                                                                       gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:752815/1‑250 (MQ=255)                                                                                                                                                                                        tCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:503538/250‑1 (MQ=255)                                                                                                                                                                                        tCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:864185/250‑1 (MQ=255)                                                                                                                                                                                          tATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCt                     >  1:200100/1‑250 (MQ=255)                                                                                                                                                                                             ttaaataatccgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAAc                 <  1:264823/226‑1 (MQ=11)                                                                                                                                                                                              taaataatccgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg                <  1:189453/227‑1 (MQ=11)                                                                                                                                                                                               aaataatccgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg                >  1:377928/24‑250 (MQ=11)                                                                                                                                                                                                 ataatccgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGcc              >  1:4314/22‑250 (MQ=11)                                                                                                                                                                                                    gAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            >  1:634811/1‑233 (MQ=255)                                                                                                                                                                                                    gAACGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTaa          >  1:843898/1‑251 (MQ=255)                                                                                                                                                                                                       cgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt       <  1:768512/236‑1 (MQ=11)                                                                                                                                                                                                       cgctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt       <  1:456693/236‑1 (MQ=11)                                                                                                                                                                                                       cGAAAATAATCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt       <  1:22249/251‑1 (MQ=255)                                                                                                                                                                                                         ctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg     <  1:731158/238‑1 (MQ=11)                                                                                                                                                                                                         ctttccgaaacaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg     <  1:327616/238‑1 (MQ=11)                                                                                                                                                                                                            ataatCGGTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGa  <  1:235117/251‑1 (MQ=25)                                                                                                                                                                                                                  ggTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                              >  1:303474/1‑201 (MQ=18)                                                                                                                                                                                                                  acaaaaaaGTGATCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAAc                 <  1:73785/226‑1 (MQ=11)                                                                                                                                                                                                                   gTTAAAAGTGATCTG‑‑CCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:728771/223‑1 (MQ=18)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            AATTGCTGTTCCATTATCGTGCCAGACATTTCTATCGTACATTGAACCGTGCGGAACAAATTAAATGGCAAAAATACTGCCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGCTCTG‑‑CACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGA  >  1/1067311‑1067764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑