Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,064,445	T→C	V1266A (GTA→GCA)	unknown →	Chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,064,445	0	T	C	100.0%	212.1 / NA	74	V1266A (GTA→GCA)	unknown	Chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/41);  total (33/41)

TTGCCGATAACGAATATCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGTAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATTATATC  >  1/1064205‑1064672                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    ttGCCGATAACGAATATCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATg                                                                                                                                                                                                                           <  1:300290/251‑1 (MQ=255)    ccGATAACGAATATCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCg                                                                                                                                                                                                                         <  1:481219/250‑1 (MQ=255)               tatCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAAc                                                                                                                                                                                                                   >  1:655343/1‑245 (MQ=255)               tatCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGG‑AAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCaa                                                                                                                                                                                                            >  1:555447/1‑251 (MQ=255)                           gCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACaaaa                                                                                                                                                                                                 >  1:92520/1‑251 (MQ=255)                           gCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACaaaa                                                                                                                                                                                                 >  1:877311/1‑251 (MQ=255)                            ccTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTAt                                                                                                                                                                                                                            <  1:368507/223‑1 (MQ=255)                            ccTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAAt                                                                                                                                                                                                <  1:235393/251‑1 (MQ=255)                                 cGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAAc                                                                                                                                                                                                                   <  1:572935/227‑1 (MQ=255)                                       tCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAAtcgtattcgtat                                                                                                                                                                                     >  1:771724/1‑251 (MQ=255)                                          gAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTGCGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAGCCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   <  1:598290/250‑1 (MQ=255)                                          gAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATg                                                                                                                                                                                    >  1:172503/1‑249 (MQ=255)                                          gAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   <  1:341541/250‑1 (MQ=255)                                                   cGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAgg                                                                                                                                                                         <  1:846771/251‑1 (MQ=255)                                                           ggAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAgg                                                                                                                                                                         >  1:138072/1‑243 (MQ=255)                                                            gAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCaaaa                                                                                                                                                                >  1:215298/1‑251 (MQ=255)                                                                aTAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGTGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   >  1:25188/1‑228 (MQ=255)                                                                aTAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCaa                                                                                                                                                                           <  1:670063/236‑1 (MQ=255)                                                                aTAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAatat                                                                                                                                                             >  1:575113/1‑250 (MQ=255)                                                                          gTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACg                                                                                                                                                                                                          >  1:521743/1‑195 (MQ=255)                                                                           ttCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAGCCGACGGCCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTc                                                                                                                                                  <  1:298048/250‑1 (MQ=255)                                                                            tCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAAc                                                                                                                                                                                                     >  1:111074/1‑198 (MQ=255)                                                                             cTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACg                                                                                                                                                                                                          <  1:861514/192‑1 (MQ=255)                                                                                    gCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAAt                                                                                                                                         >  1:738730/1‑250 (MQ=255)                                                                                     cGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATc                                                                                                                                        <  1:761191/250‑1 (MQ=255)                                                                                     cGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATc                                                                                                                                        <  1:468803/250‑1 (MQ=255)                                                                                     cGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCACTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATc                                                                                                                                        <  1:609808/250‑1 (MQ=255)                                                                                       gTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   >  1:477526/1‑205 (MQ=255)                                                                                         ataCCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTg                                                                                                                                    >  1:149287/1‑250 (MQ=255)                                                                                         ataCCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTg                                                                                                                                    >  1:814214/1‑250 (MQ=255)                                                                                         ataCCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTg                                                                                                                                    >  1:213866/1‑250 (MQ=255)                                                                                            ccAACACCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   <  1:503669/200‑1 (MQ=255)                                                                                                cATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAAt                                                                                                                                                                                                >  1:496781/1‑183 (MQ=255)                                                                                                cATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTtggt                                                                                                                            <  1:898268/251‑1 (MQ=255)                                                                                                 aTCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTc                                                                                                                                                                                                              >  1:63596/1‑168 (MQ=255)                                                                                                 aTCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGcc                                                                                                                                                         >  1:229852/1‑221 (MQ=255)                                                                                                   cTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGc                                                                                                                                                                                   <  1:386087/193‑1 (MQ=255)                                                                                                        cgAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAat                                                                                                                     <  1:95875/250‑1 (MQ=255)                                                                                                          aaCGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAgg                                                                                                                                               >  1:823711/1‑222 (MQ=255)                                                                                                          aaCGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAatat                                                                                                                   <  1:106154/250‑1 (MQ=255)                                                                                                              tATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTg                                                                                                              <  1:279615/251‑1 (MQ=255)                                                                                                              tATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTg                                                                                                              <  1:100139/251‑1 (MQ=255)                                                                                                                 ccGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATa                                                                                                           <  1:277272/251‑1 (MQ=255)                                                                                                                  cGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATc                                                                                                                                        <  1:647711/221‑1 (MQ=255)                                                                                                                  cGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTtg                                                                                                                              >  1:747125/1‑231 (MQ=255)                                                                                                                           atTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTg                                                                                                                                             <  1:482166/207‑1 (MQ=255)                                                                                                                            ttCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAatat                                                                                                                   >  1:512856/1‑232 (MQ=255)                                                                                                                             tCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGAt                                                                                                            <  1:480975/238‑1 (MQ=255)                                                                                                                              cTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCAtgtt                                                                                              <  1:733894/251‑1 (MQ=255)                                                                                                                              cTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCAtgtt                                                                                              <  1:709697/251‑1 (MQ=255)                                                                                                                               tGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCAtgttg                                                                                             <  1:75114/251‑1 (MQ=255)                                                                                                                                               gATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCg                                                                             >  1:536514/1‑251 (MQ=255)                                                                                                                                                            gAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGa                                                                >  1:209258/1‑251 (MQ=255)                                                                                                                                                               cAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGAt                                                               <  1:396399/249‑1 (MQ=255)                                                                                                                                                                 aaTGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATc                                                           <  1:18738/251‑1 (MQ=255)                                                                                                                                                                   tGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAg                                                         <  1:308597/251‑1 (MQ=255)                                                                                                                                                                      aaaTCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATAtt                                                                                                                  <  1:900392/191‑1 (MQ=255)                                                                                                                                                                      aaaTCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGat                                                      <  1:494935/251‑1 (MQ=255)                                                                                                                                                                           gAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGatttatt                                                 >  1:144856/1‑251 (MQ=255)                                                                                                                                                                              cTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTAc                                              <  1:710788/251‑1 (MQ=255)                                                                                                                                                                              cTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAAGCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGTGCTTTCCGGCAACCAATCGGATTATCAGGatttattt                                                <  1:581966/249‑1 (MQ=255)                                                                                                                                                                                ttCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATAtt                                                                                                                  >  1:19220/1‑181 (MQ=255)                                                                                                                                                                                     gCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAAt                                       >  1:860072/1‑251 (MQ=255)                                                                                                                                                                                      cTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAGCCAATCGGATTATCAGGATTTATTTACCGACAAt                                       <  1:572020/250‑1 (MQ=255)                                                                                                                                                                                        tAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTc                                                                                                                                                  >  1:576628/1‑141 (MQ=255)                                                                                                                                                                                        tAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGt                                    >  1:615323/1‑251 (MQ=255)                                                                                                                                                                                          aCGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCAtgttgt                                                                                            >  1:376405/1‑193 (MQ=255)                                                                                                                                                                                                 agTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACtttt                           >  1:478890/1‑251 (MQ=255)                                                                                                                                                                                                   ttAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTc                         <  1:682760/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCTGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATta      >  1:178159/1‑251 (MQ=255)                                                                                                                                                                                                                         tGGCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATTatat   <  1:238846/251‑1 (MQ=255)                                                                                                                                                                                                                           gCGATCAGCTCTGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATTATATc  <  1:705290/250‑1 (MQ=255)                                                                                                                                                                                                                                   ctctGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAgg                                                        <  1:726180/188‑1 (MQ=255)                                                                                                                                                                                                                                   ctctGAAAGCGTGGCAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATTatat   <  1:307387/241‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    TTGCCGATAACGAATATCTGCGCGATGCCTTACGTTTATCGGAAGACAGTCGCAAACCGGAAAATAAAGTGCGGTTCTTTATTGCGGTATACCAACATCTACGCGAACGTATCCGTCAGGATATTCTGAAAACCGACGACCCGATTGATGCCATCGAGCAAATGGAAATCGAGCTTTCCCGCTTAACGGAAGAGTTAACCGGTCGTGAACAGAAACTGGCGATCAGCTCTGAAAGCGTGGTAAACATTATGCGTAAAACCATTCAACGTGAACAAAATCGTATTCGTATGCTGAATCAAGGCTTGCAAAATATCGCCTTCGGTCAGGTGAAATCAGTGCGTTTGGTGGTGAATATTCGTGATACGCACGCCATGTTGTTGGATGCGCTTTCCGGCAACCAATCGGATTATCAGGATTTATTTACCGACAATCGTATGACTTTTTCCGAGGCTATTGCGAAATTATATC  >  1/1064205‑1064672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑