Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,063,202	T→C	Y852H (TAT→CAT)	unknown →	Chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,063,202	0	T	C	100.0%	196.4 / NA	68	Y852H (TAT→CAT)	unknown	Chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/38);  total (30/38)

TTTGATGATAGCGTACTTTCCGCACAGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTATATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTGAAGAATGCCGCGAACAGCTGGATTTAGCCGA  >  1/1062959‑1063446                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tttGATGATAGCGTACTTTCCGCACAGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAg                                                                                                                                                                                                                                                >  1:188/1‑250 (MQ=255)        atAGCGTACTTTCCGCACAGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGc                                                                                                                                                                                                                                               <  1:95811/244‑1 (MQ=255)                 tttCCGCACAGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGAt                                                                                                                                                                                                                               <  1:728608/251‑1 (MQ=255)                       cacaGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATcc                                                                                                                                                                                                                          <  1:389674/250‑1 (MQ=255)                           ggAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGaa                                                                                                                                                                                                                      >  1:96709/1‑250 (MQ=255)                           ggAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGaa                                                                                                                                                                                                                      >  1:257943/1‑250 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:695131/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:678686/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     >  1:190120/1‑250 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:197658/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:711711/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:120174/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:560987/250‑1 (MQ=255)                            gAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTAACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:648311/250‑1 (MQ=255)                             aaTTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:676314/249‑1 (MQ=255)                                       gggCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGAt                                                                                                                                                                                                                               <  1:618559/229‑1 (MQ=255)                                            ttGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGAcc                                                                                                                                                                                                    <  1:243760/251‑1 (MQ=255)                                            ttGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGAc                                                                                                                                                                                                     <  1:709043/250‑1 (MQ=255)                                              gtggtgCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCaa                                                                                                                                                                                                  >  1:365435/1‑251 (MQ=255)                                              gtggtgCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCaa                                                                                                                                                                                                  >  1:645783/1‑251 (MQ=255)                                                              aTCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAg                                                                                                                                                                                                                                          <  1:646349/195‑1 (MQ=255)                                                              aTCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGAt                                                                                                                                                                                                                               >  1:17809/1‑206 (MQ=255)                                                                cGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACAttt                                                                                                                                                                                                                                                  >  1:709550/1‑185 (MQ=255)                                                                  tGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTg                                                                                                                                                                              <  1:272251/251‑1 (MQ=255)                                                                        gCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:776376/206‑1 (MQ=255)                                                                             aTTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGAc                                                                                                                                                                                                     >  1:573826/1‑217 (MQ=255)                                                                             aTTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTa                                                                                                                                                                   >  1:601688/1‑251 (MQ=255)                                                                                cTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGGGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAAc                                                                                                                                                                <  1:424573/251‑1 (MQ=255)                                                                                                      gttTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATc                                                                                                                                                                                                >  1:874132/1‑197 (MQ=255)                                                                                                           gTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAAtt                                                                                                                                     >  1:154533/1‑251 (MQ=255)                                                                                                            tCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTCGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTc                                                                                                                                    <  1:440340/251‑1 (MQ=255)                                                                                                               tGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATcagcag                                                                                                                                                                                           >  1:857840/1‑193 (MQ=255)                                                                                                                      cGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCt                                                                                                                                                                                                          <  1:569920/171‑1 (MQ=255)                                                                                                                          aaaaaCATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGgat                                                                                                                      <  1:214658/251‑1 (MQ=255)                                                                                                                                                ggCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:519931/134‑1 (MQ=255)                                                                                                                                                ggCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAgt                                                                                                >  1:164147/1‑251 (MQ=255)                                                                                                                                                 gCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATTAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAg                                                                                                 <  1:490610/249‑1 (MQ=255)                                                                                                                                                 gCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAg                                                                                                 <  1:185170/249‑1 (MQ=255)                                                                                                                                                       cGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     >  1:312453/1‑127 (MQ=255)                                                                                                                                                       cGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAAc                                                                                                                                                                                                                     <  1:685436/127‑1 (MQ=255)                                                                                                                                                        gTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAg                                                                                                            >  1:704367/1‑231 (MQ=255)                                                                                                                                                             gAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatt                                                                                   >  1:626821/1‑251 (MQ=255)                                                                                                                                                              aaGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatt                                                                                   <  1:203796/250‑1 (MQ=255)                                                                                                                                                                 tGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATca                                                                                                                                                                                               <  1:141523/139‑1 (MQ=255)                                                                                                                                                                 tGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTcc                                                                                                                                                         >  1:237042/1‑177 (MQ=255)                                                                                                                                                                   gCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTc                                                                              >  1:207396/1‑250 (MQ=255)                                                                                                                                                                      gAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCg                                                                                                                                                                                                                                        <  1:607493/93‑1 (MQ=255)                                                                                                                                                                      gAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCa                                                                          >  1:359639/1‑251 (MQ=255)                                                                                                                                                                        aCATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGc                                                                                                                                                                                                                                               >  1:359454/1‑84 (MQ=255)                                                                                                                                                                          aTTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTg                                                                      <  1:555312/251‑1 (MQ=255)                                                                                                                                                                                         cTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGAt                                                                                                                                                                                                                               >  1:4470/1‑83 (MQ=255)                                                                                                                                                                                         cTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGAc                                                                                                                                                                                                     <  1:633782/109‑1 (MQ=255)                                                                                                                                                                                         cTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatt                                                                                   >  1:241866/1‑223 (MQ=255)                                                                                                                                                                                         cTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGAt                                                       >  1:774790/1‑251 (MQ=255)                                                                                                                                                                                                cGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGtt                                                >  1:791751/1‑251 (MQ=255)                                                                                                                                                                                                    cAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatt                                                                                   <  1:741945/212‑1 (MQ=255)                                                                                                                                                                                                    cAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAAc                                            <  1:543895/251‑1 (MQ=255)                                                                                                                                                                                                    cAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTCAAc                                            <  1:462366/251‑1 (MQ=255)                                                                                                                                                                                                      aaaaTGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCg                                          <  1:429432/251‑1 (MQ=255)                                                                                                                                                                                                               gCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaattaat                                                                                <  1:262819/204‑1 (MQ=255)                                                                                                                                                                                                               gCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTg                                 >  1:177497/1‑251 (MQ=255)                                                                                                                                                                                                                    tACACGAACATTTCAGCCAATTTGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatta                                                                                  <  1:132592/197‑1 (MQ=255)                                                                                                                                                                                                                                        tttGTCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTgg                                                            >  1:719718/1‑199 (MQ=255)                                                                                                                                                                                                                                            tCGGTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATCCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTGAAGAATGCCGCGAACAGCTGGATTTAGc     <  1:282186/250‑1 (MQ=255)                                                                                                                                                                                                                                              ggTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAAt                                                                                        <  1:858778/165‑1 (MQ=255)                                                                                                                                                                                                                                              ggTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTGAAGAATGCCGCGAACAGCTGGATTTAGc     >  1:773915/1‑248 (MQ=255)                                                                                                                                                                                                                                              ggTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTGAAGAATGCCGCGAACAGCTGGATTTAGCCGa  >  1:765862/1‑251 (MQ=255)                                                                                                                                                                                                                                               gTTTACATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAaatt                                                                                   >  1:690933/1‑169 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TTTGATGATAGCGTACTTTCCGCACAGGAATTGGAACTGGGCGTTGTGGTGCAAGTGTCCGATCGTGAATTGCGTTATTCTAAATTCCCGGAAATTCCGTTGTTTGGTCGTGCCGCTCGTGAAAAACATTTGGAAGAATTACAGGCAAAACGTGAGGAAGTGGCGGAACATTACGCTCAACGCGCTTTTGACGTGCAAAAATGCCAGCGTTTACACGAACATTTCAGCCAATTTGTCGGTTTATATTTAGCATTAGCGTTCCAAGATAATCCAGAACAGGTCATGGCTCGGACCAATCAGCAGCGCAATGAAATTGAACGCGAACTAAACCAATTCCTGACCGGCGAACAACAAATTCGCATTCGTTTGGATGATGCGAAAGAACGTATGCAGTTGTTGAATAAATTAATTCCGCAATTGCCATTATTGGCGGATGACAGTTTAACCGATCGCATTGAAGAATGCCGCGAACAGCTGGATTTAGCCGA  >  1/1062959‑1063446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑