Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,060,433	T→C	S175S (AGT→AGC)	unknown →	Chromosome partition protein MukE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,060,433	0	T	C	100.0%	162.1 / NA	58	S175S (AGT→AGC)	unknown	Chromosome partition protein MukE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/27);  total (31/27)

GGCAAAGTGTTGTGTTATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGTGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATCCCTGGAAAAT  >  1/1060191‑1060675                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ggCAAAGTGTTGTGTTATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAAt                                                                                                                                                                                                                                             <  1:219294/250‑1 (MQ=255) ggCAAAGTGTTGTGTTATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAAt                                                                                                                                                                                                                                             <  1:235905/250‑1 (MQ=255)       gtgttgtgttATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCAt                                                                                                                                                                                                                                      >  1:627030/1‑251 (MQ=255)            gtgttATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCg                                                                                                                                                                                                                                 >  1:465866/1‑251 (MQ=255)                 aTTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATc                                                                                                                                                                                                                             >  1:177912/1‑250 (MQ=255)                       aTTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTc                                                                                                                                                                                                                      <  1:680521/251‑1 (MQ=255)                          tAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCg                                                                                                                                                                                                                    >  1:179035/1‑250 (MQ=255)                             gCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCttt                                                                                                                                                                                                                >  1:655693/1‑251 (MQ=255)                                      gCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGaa                                                                                                                                                                                                       >  1:203976/1‑251 (MQ=255)                                                gcagGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCg                                                                                                                                                                                             >  1:722570/1‑251 (MQ=255)                                                gcagGGGATTTTCAGCACCCAGGAAGTGTATGACGAACCGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTAcc                                                                                                                                                                                                                                        <  1:428692/208‑1 (MQ=255)                                                          ttCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATc                                                                                                                                                                                    >  1:643503/1‑250 (MQ=255)                                                               cACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTg                                                                                                                                                                              >  1:279165/1‑251 (MQ=255)                                                                   cAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCga                                                                                                                                                                                         <  1:811435/236‑1 (MQ=255)                                                                         gtgtATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAAtt                                                                                                                                                                    >  1:638448/1‑251 (MQ=255)                                                                         gtgtATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAAtt                                                                                                                                                                    >  1:701358/1‑251 (MQ=255)                                                                          tgtATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAAt                                                                                                                                                                     <  1:69687/249‑1 (MQ=255)                                                                                    aCTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTc                                                                                                                                                         <  1:805601/251‑1 (MQ=255)                                                                                       gTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTg                                                                                                                                                      >  1:882584/1‑251 (MQ=255)                                                                                         tGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGAt                                                                                                                                                    <  1:516434/251‑1 (MQ=255)                                                                                          gAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAAATGGCGGGAAAAGTGCGGGCGGCGATCGGTCGTTTGCGGCGTTTAGGCATTATTTATACGGTTGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCg                                                                                                                                                        <  1:122408/246‑1 (MQ=255)                                                                                             tttAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTg                                                                                                                                                <  1:674080/251‑1 (MQ=255)                                                                                                  ccGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGc                                                                                                                                            <  1:738865/250‑1 (MQ=255)                                                                                                    gATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCgg                                                                                                                                          >  1:681454/1‑250 (MQ=255)                                                                                                                  gCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGAtt                                                                                                                                                                               <  1:378655/199‑1 (MQ=255)                                                                                                                      aaGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTAc                                                                                                                        <  1:791986/250‑1 (MQ=255)                                                                                                                        ggCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAgg                                                                                                                     >  1:535096/1‑251 (MQ=255)                                                                                                                          cGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGc                                                                                                                    <  1:175905/250‑1 (MQ=255)                                                                                                                                 tCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAAc                                                                                                            <  1:357329/251‑1 (MQ=255)                                                                                                                                  cAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGaaaaa                                                                                                             >  1:553556/1‑249 (MQ=255)                                                                                                                                       ttCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGt                                                                                                      >  1:200839/1‑251 (MQ=255)                                                                                                                                       ttCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGt                                                                                                      >  1:643795/1‑251 (MQ=255)                                                                                                                                          cAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTa                                                                                                    >  1:860075/1‑250 (MQ=255)                                                                                                                                            gCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACaa                                                                                                 >  1:43323/1‑251 (MQ=255)                                                                                                                                                         ggATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGaaa                                                                                    >  1:553557/1‑251 (MQ=255)                                                                                                                                                              aaCAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCg                                                                                                       <  1:147712/227‑1 (MQ=255)                                                                                                                                                                    aaTTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAAc                                                                         <  1:764071/251‑1 (MQ=255)                                                                                                                                                                            gAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACg                                                                                                                                                                 >  1:863076/1‑155 (MQ=255)                                                                                                                                                                                   tgcgtgcgGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGgaa                                                          <  1:331001/251‑1 (MQ=255)                                                                                                                                                                                       tgcgGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGgaagaa                                                       <  1:742414/250‑1 (MQ=255)                                                                                                                                                                                         cggcgATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGgaagaagaa                                                    <  1:170723/251‑1 (MQ=255)                                                                                                                                                                                           gcgATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGt                                                  >  1:706167/1‑251 (MQ=255)                                                                                                                                                                                                      tttGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAgg                                       >  1:431611/1‑251 (MQ=255)                                                                                                                                                                                                              gtTTAGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGgaa                                                          <  1:634685/224‑1 (MQ=255)                                                                                                                                                                                                                  aGGCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTagaa                                           <  1:885451/235‑1 (MQ=255)                                                                                                                                                                                                                   ggCATGATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAAtt                                                                                                                                                          >  1:714804/1‑123 (MQ=255)                                                                                                                                                                                                                        gATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGgaagaagaa                                                    <  1:722141/220‑1 (MQ=255)                                                                                                                                                                                                                        gATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAgg                                       <  1:303465/233‑1 (MQ=255)                                                                                                                                                                                                                        gATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTga                     >  1:422354/1‑251 (MQ=255)                                                                                                                                                                                                                        gATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTga                     >  1:28536/1‑251 (MQ=255)                                                                                                                                                                                                                        gATTTATACGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGCGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTga                     >  1:838306/1‑251 (MQ=255)                                                                                                                                                                                                                             ataCGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAAt                <  1:701321/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGGTGGGTGAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACa                                                                                                                       <  1:451477/146‑1 (MQ=255)                                                                                                                                                                                                                                  gtgggtgAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATc            <  1:707992/250‑1 (MQ=255)                                                                                                                                                                                                                                    gggtgAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATcc           >  1:305729/1‑249 (MQ=255)                                                                                                                                                                                                                                    gggtgAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATcc           >  1:291330/1‑249 (MQ=255)                                                                                                                                                                                                                                        gAACCGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAat                                                                                              >  1:554205/1‑162 (MQ=255)                                                                                                                                                                                                                                           ccGAACAGCGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATCCCTGGAAAAt  >  1:751748/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GGCAAAGTGTTGTGTTATTTGTATTTAAGCCCGGAACGCCTGGCACAGCAGGGGATTTTCAGCACCCAGGAAGTGTATGACGAACTGTTGAATTTAGCCGATGAAAGTAAATTGCTGAAGGCGGTGAATCAGCGTTCCAGCGGTTCCGATTTGGATAAACAAAAATTGGCGGAAAAAGTGCGTGCGGCGATCGGTCGTTTGCGTCGTTTAGGCATGATTTATACGGTGGGTGAACCGAACAGTGGTAAATTTACCATTTCCGAATCCGTGTTCCGCTTTGGTGCCGAAGTGCGCGCCGGCGATGATCCGATTGAAGCGCAATTACGTTTAATTCGTGATGGTGAAGCGGCAACCCCGCAATCCTTACAGGCAGAAAAACTGGCGTTACAAAATGATGCCGAAAACAGCGAAAACGATGCGGAAGTGGAAGAAGAAGTCGTAGAAGAGGAACAAGAATAATGACTGAAGAATTATCCCTGGAAAAT  >  1/1060191‑1060675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑