Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 112,818 T→C V242A (GTA→GCA)  unknown → Triosephosphate isomerase (EC 5.3.1.1)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1112,8180TC99.1% 305.0 / NA 113V242A (GTA→GCA) unknownTriosephosphate isomerase (EC 5.3.1.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (49/63);  minor base G (1/0);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CCTACGAACCGATTTGGGCAATCGGCACCGGGAAATCTGCCACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGTATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTACCACAAATC  >  1/112590‑113061
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
ccTACGAACCGATTTGGGCAATCGGCACCGGGAAATCTGCCACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAagc                                                                                                                                                                                                                                 <  1:104460/250‑1 (MQ=255)
ccTACGAACCGATTTGGGCAATCGGCACCGGGAAATCTGCCACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAagc                                                                                                                                                                                                                                 <  1:128118/250‑1 (MQ=255)
                                aaaTCTGCCACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACCCGGC‑GGAATTGTTTACCCCACCGGGTATTTACGGGG‑CATTGGGGGGGGGCGC‑GGCCTTTAAAACCCCCGCCTTTTCGGGTTTCGTAAAAACAGCGGCAAAAACGGAAAACTAAATTTCCttctt                                                                                                                                                                                                >  1:186182/1‑248 (MQ=255)
                                       ccACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTGCTGCTc                                                                                                                                                                                         <  1:694160/250‑1 (MQ=255)
                                               gcgcaagcgcaaGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTTCGCAAACAACGCCACAGCGGG‑GGGATTGCTCACCCCACCAGATATTTTCGGGGTGATGGCTGGG‑GGCGC‑GGCCTTAAAAACCCCGGGATTTGCGGGTATCGTAAAAACAGCCGCCAAAGCGAAAAAAT‑AATTTCCTTTTTTCTTCCCAGgggcggg                                                                                                                                                                                >  1:550355/1‑250 (MQ=255)
                                                      cgcaagcgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGc                                                                                                                                                                           <  1:509961/249‑1 (MQ=255)
                                                          agcgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTTCCTAAACGATGCCAACCCGGC‑GGGATTGTTTACCCAACCGGGTATTTAAGGGG‑CATTGGGGGGCGGCGCGGCCCTTAAAAG‑CCCGGCCTTTGCGGGTATCGGAAAAACAGCGGCAAAAAAGAAAAACT‑AAATTTCTTTTTTCTGCTCCGGGGCGGGCAAGCCCAcc                                                                                                                                                                      >  1:404664/1‑250 (MQ=255)
                                                           gcgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGc                                                                                                                                                                                                                           <  1:764028/197‑1 (MQ=255)
                                                            cgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTTCGGAAACGATGCCAAACCGGC‑GGGATTTTTCACCCCAACGGGTATTTACGGGG‑CATTGGGGGGGGGCGC‑GGCCTTAAAAACCCCCGCAATTGCGGGTTTCGTAAAAACAGCGGCAAAAACGAAAAAAC‑AAATTCCTTTCTTTTTCTCCGGGGTGGTCAAGCCGAccc                                                                                                                                                                     >  1:748074/1‑249 (MQ=255)
                                                            cgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGc                                                                                                                                                                           <  1:162381/243‑1 (MQ=255)
                                                            cgGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGAccc                                                                                                                                                                     <  1:177303/249‑1 (MQ=255)
                                                              gTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCCACGCGGC‑GGGATTGTTCACCCCACCCGATATTGACGGGG‑CATTGGTGGGCGGCGC‑GTCCTTTAAAACCCCGGCATTTTCGGGTATCGTAAAAGCAGCGGCAAAAACGAAAAACT‑AATTTTCTTTCTTCTGCTCAGGGGCGGTCCAGCCGACCCtttt                                                                                                                                                                 >  1:483552/1‑251 (MQ=255)
                                                              gTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGGATTTTTTACCCAACCGGGTATTGACGGTG‑CATTGGGGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTTCGGTTATCGTAAAAACAGCGGCAAAAGCGAAAAACT‑AATTTTCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCcttt                                                                                                                                                                 >  1:495270/1‑251 (MQ=255)
                                                                     cgTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACTGCGGTTTCGTAAACTATTCCCACCCGGC‑GGTATTTTTTACCCCACCCGGTATTTACGGTT‑CATTTGTTGGCGGCGC‑GTCCTTTAAAACCCCTGCCTTTTCGGTTTTTTTTAAAACAGCCGCCCAAACGAAAAACT‑AATTTCCTTTCTTCTTCTCCGGGTCTGTTAAGCCGAACCttttttttttt                                                                                                                                                          >  1:422931/1‑250 (MQ=255)
                                                                     cgTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTTACCCCACCCGATATTTACGGGG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCCGCATTTGCGGGTATCGTAAAAGCAGCGGCAAAAACGAAAAACT‑AATTTCCTTTCTTCTTCTCAGGGTCGGTCAAGCCGACCCTTTTTGttttt                                                                                                                                                          >  1:779530/1‑250 (MQ=255)
                                                                     cgTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACCCGGC‑GGGATTGTTTACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAACCCCGGCCTTTTCGGGTATCGTAAAAACAGCGGCAAAAGCGAAAAAAT‑AATTTCCTTTCTTCTGCTCAGGGGCGGGCAAGCCGACCCTTTTTGttttt                                                                                                                                                          >  1:441482/1‑250 (MQ=255)
                                                                           ttCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGggt                                                                                                                                                                                    <  1:566456/219‑1 (MQ=255)
                                                                               cgGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACCCGGC‑GGAATTGTTCACCCCACCGGATATTTACGGGG‑CCTTGGGGGGCGGCGC‑GGCCTTAAAAGCCCCCGCATTTGCGGTTATCGTAAAAACAGCGGCAAAAGCGAAAAACT‑AATTTTCTTTCTTCTTCTCAGGGTCGGGCAAGCCGACCCTTTTTTTTTTCATATTTccca                                                                                                                                                >  1:650959/1‑248 (MQ=255)
                                                                                             aaaaaTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATaa                                                                                                                                  <  1:248342/251‑1 (MQ=255)
                                                                                             aaaaaTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATaa                                                                                                                                  <  1:600934/251‑1 (MQ=255)
                                                                                             aaaaaTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATaa                                                                                                                                  <  1:210942/251‑1 (MQ=255)
                                                                                             aaaaaTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATaa                                                                                                                                  <  1:783217/251‑1 (MQ=255)
                                                                                               aaaTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATg                                                                                                                                <  1:627972/251‑1 (MQ=255)
                                                                                                 aTCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTttcttc                                                                                                                                                                                              <  1:214423/187‑1 (MQ=255)
                                                                                                   cccAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGGG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAACAGCGGCAAAAGCGAAAAAAT‑AATTTCCTTTCTTCTGCTCAGGGGCGGTCAAGCCGACCCTTTTTGTTTTTAGATTTTACAAACTTCCGCTATAATGACCg                                                                                                                            >  1:97785/1‑251 (MQ=255)
                                                                                                       aGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAAAGATGCCAACGCGGCGGGAATT‑TTTACCCCACCGGGTATTTACGGGG‑CATTGGGGGGCGGCGCGGCCCTTAAAA‑CCCCCGCATTTTGGGTTTTCGTAAAAACAGCGGCAAAAACGAAAAAAT‑AATTTTCTTTCTTTTGCCCCGGGGCGGGCAAACCGACCCCTTTTTTTTTCAGATTTTTCAAACTTCCGCTATAATTACCGccc                                                                                                                         >  1:497185/1‑248 (MQ=255)
                                                                                                        gCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTc                                                                                                                                                                                         <  1:116706/185‑1 (MQ=255)
                                                                                                         cGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACttt                                                                                                                      <  1:265295/251‑1 (MQ=255)
                                                                                                              gCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGGATTGTTCACCCAACCGGATATTTACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAACCCCCGCATTTTCGGGTATCGTAAAAGCAGCGGCAAAAACGAAAAACT‑AATTTCCTttcttc                                                                                                                                                                                              >  1:326504/1‑174 (MQ=255)
                                                                                                                cGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAAcc                                                                                                               <  1:896246/251‑1 (MQ=255)
                                                                                                                  aTCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑TATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCtt                                                                                                             <  1:430979/251‑1 (MQ=255)
                                                                                                                      aGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGGATTGTTCACCCCACCGGATATTGACGGGG‑CATTTGTGGGCGGCGC‑GTCCTTAAAAACCCCCGCATTTTCGGTTATCGTAAAAGCAGCGGCAAAAACGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGGCAAGcc                                                                                                                                                                          >  1:747820/1‑186 (MQ=255)
                                                                                                                        ttattaTTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTc                                                                                                                                                                                         <  1:12930/169‑1 (MQ=255)
                                                                                                                        ttattaTTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGggt                                                                                                                                                                                    <  1:871975/174‑1 (MQ=255)
                                                                                                                        ttattaTTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAAttt                                                                                                        <  1:549971/250‑1 (MQ=255)
                                                                                                                        ttattaTTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAAttt                                                                                                        <  1:306445/250‑1 (MQ=255)
                                                                                                                          attattCAATACGGCGGTTCCGTAAAAGATGCCAACGCGGCGGGAATT‑TTCACCCAACCGGATATTGACGGGG‑CATTGGTGGGCGGCGC‑GGCCTTAAAAACCCCGGCATTTGCGGGTATCGTAAAAACAGCGGCAAAAACGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTTAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAAtttctt                                                                                                     >  1:490554/1‑248 (MQ=255)
                                                                                                                            tattCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAgg                                                                                                   <  1:870562/251‑1 (MQ=255)
                                                                                                                             attCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGAcc                                                                                                                             <  1:852555/224‑1 (MQ=255)
                                                                                                                                    aCGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTtctc                                                                                           <  1:697814/251‑1 (MQ=255)
                                                                                                                                          gTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCta                                                                                                                                     <  1:238078/203‑1 (MQ=255)
                                                                                                                                            tCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCATCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATaa                                                                                                                                  <  1:451645/204‑1 (MQ=255)
                                                                                                                                            tCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCaaaa                                                                                   >  1:855121/1‑251 (MQ=255)
                                                                                                                                            tCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCaaaa                                                                                   >  1:818613/1‑251 (MQ=255)
                                                                                                                                            tCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGGTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACCAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATgtacaa                                                                                   >  1:35119/1‑246 (MQ=255)
                                                                                                                                             ccGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGcc                                                                                                                                                                          >  1:575986/1‑163 (MQ=255)
                                                                                                                                             ccGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAAt                                                                                  >  1:518166/1‑251 (MQ=255)
                                                                                                                                                    cGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGggt                                                                                                                                                                                    >  1:723604/1‑146 (MQ=255)
                                                                                                                                                      aTGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAgat                                                                         >  1:785022/1‑251 (MQ=255)
                                                                                                                                                           aaCGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGAtgt                                                                    <  1:135574/251‑1 (MQ=255)
                                                                                                                                                                  c‑ggAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTa                                                              <  1:751687/250‑1 (MQ=255)
                                                                                                                                                                     gAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATg                                                            <  1:346756/250‑1 (MQ=255)
                                                                                                                                                                      aaTTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATgc                                                           <  1:669200/250‑1 (MQ=255)
                                                                                                                                                                       aTTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTg                                                                                                                                                                                                                                                 >  1:195407/1‑68 (MQ=255)
                                                                                                                                                                        ttGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAAc                                                                                                                <  1:171658/195‑1 (MQ=255)
                                                                                                                                                                                  aaCCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAAttt                                                                                                        >  1:217764/1‑193 (MQ=255)
                                                                                                                                                                                   aCCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCATTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGga                                             >  1:388895/1‑251 (MQ=255)
                                                                                                                                                                                   aCCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTgg                                              <  1:494923/250‑1 (MQ=255)
                                                                                                                                                                                    ccGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgat                                            <  1:254983/251‑1 (MQ=255)
                                                                                                                                                                                     cGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgatc                                           <  1:320832/251‑1 (MQ=255)
                                                                                                                                                                                       gATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAGGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAg                                                                           <  1:40704/217‑1 (MQ=255)
                                                                                                                                                                                       gATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCCCTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgatcga                                         >  1:423116/1‑251 (MQ=255)
                                                                                                                                                                                       gATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgatcga                                         >  1:707832/1‑251 (MQ=255)
                                                                                                                                                                                         tatTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAAc                                                                                                                >  1:729610/1‑178 (MQ=255)
                                                                                                                                                                                         tatTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgatcgatc                                       <  1:457512/251‑1 (MQ=255)
                                                                                                                                                                                           ttGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATcac                                     <  1:209534/251‑1 (MQ=255)
                                                                                                                                                                                           ttGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATcac                                     <  1:14046/251‑1 (MQ=255)
                                                                                                                                                                                               cGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAgcg                                 <  1:94876/251‑1 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgatcgatc                                       <  1:556102/243‑1 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgat                                            <  1:753394/238‑1 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAgcgc                                <  1:131007/250‑1 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGcgcg                               >  1:779264/1‑251 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGcgcg                               >  1:734854/1‑251 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGcgcg                               >  1:322904/1‑251 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGcgcg                               >  1:33828/1‑251 (MQ=255)
                                                                                                                                                                                                 gTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGcgcg                               >  1:260263/1‑251 (MQ=255)
                                                                                                                                                                                                   g‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTagag                                                 >  1:656026/1‑231 (MQ=255)
                                                                                                                                                                                                   g‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAgcg                                 <  1:217224/247‑1 (MQ=255)
                                                                                                                                                                                                      aTTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGAcc                                                                                                                             <  1:172144/153‑1 (MQ=255)
                                                                                                                                                                                                         ggtggGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTaca                        >  1:857358/1‑251 (MQ=255)
                                                                                                                                                                                                         ggtggGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTaca                        >  1:890322/1‑251 (MQ=255)
                                                                                                                                                                                                           tggGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTacac                       <  1:395748/250‑1 (MQ=255)
                                                                                                                                                                                                           tggGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACAcc                      <  1:72795/251‑1 (MQ=255)
                                                                                                                                                                                                           tggGCGGCGC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACAcc                      <  1:596197/251‑1 (MQ=255)
                                                                                                                                                                                                              gcggcgC‑GTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGa                    <  1:740595/250‑1 (MQ=255)
                                                                                                                                                                                                                      gTCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTag                                                   <  1:360934/212‑1 (MQ=255)
                                                                                                                                                                                                                       tCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCaa                                                                                     >  1:691329/1‑177 (MQ=255)
                                                                                                                                                                                                                       tCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgat                                            >  1:551706/1‑218 (MQ=255)
                                                                                                                                                                                                                       tCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGgat                                            >  1:502478/1‑218 (MQ=255)
                                                                                                                                                                                                                       tCCTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTa           >  1:578732/1‑251 (MQ=255)
                                                                                                                                                                                                                         cTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCtttt                                                                                                                                                                 <  1:595687/99‑1 (MQ=255)
                                                                                                                                                                                                                         cTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCt                                                                                                              >  1:90411/1‑150 (MQ=255)
                                                                                                                                                                                                                         cTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTtct                                                                                            >  1:128137/1‑168 (MQ=255)
                                                                                                                                                                                                                         cTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCggg                             >  1:65074/1‑231 (MQ=255)
                                                                                                                                                                                                                         cTTAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTAc          >  1:574160/1‑250 (MQ=255)
                                                                                                                                                                                                                          ttAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTACca        >  1:212575/1‑251 (MQ=255)
                                                                                                                                                                                                                          ttAAAAGCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTACca        <  1:332897/251‑1 (MQ=255)
                                                                                                                                                                                                                                gCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGggt                                                                                                                                                                                    <  1:345556/73‑1 (MQ=255)
                                                                                                                                                                                                                                gCCCCGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAgg                                                                                                   >  1:358244/1‑154 (MQ=255)
                                                                                                                                                                                                                                 ccccGGCATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTACCACAAATc  <  1:741802/250‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
CCTACGAACCGATTTGGGCAATCGGCACCGGGAAATCTGCCACTCCGGCGCAAGCGCAAGCGGTACACGCGTTCATTCGCGGTCATATCGCGAAAAAATCCCAAGCGGTTGCCGATCAAGTTATTATTCAATACGGCGGTTCCGTAAACGATGCCAACGCGGC‑GGAATTGTTCACCCAACCGGATATTGACGGTG‑CATTGGTGGGCGGCGC‑GTCCTTAAAAGCCCCGGTATTTGCGGTTATCGTAAAAGCAGCGGCAAAAGCGAAAAACT‑AATTTCCTTTCTTCTGCTCAGGGTCGGTCAAGCCGACCCTTTTTGTTTTCAGATTTCACAAACTTCCGCTATAATGACCGCACTTTTTTAACCTTAATTTAAAGGAATTTCTCATGCAAAATCCGAAAGATGATGTGTTTTATGCGCCGGTAGAGTGGATCGATCACAGCGCGGGCTACACCGATATTCGTTACCACAAATC  >  1/112590‑113061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑