Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,054,700	T→G	*281E (TAA→GAA)	unknown →	Murein‑DD‑endopeptidase (EC 3.4.99.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,054,700	0	T	G	100.0%	154.7 / NA	54	*281E (TAA→GAA)	unknown	Murein‑DD‑endopeptidase (EC 3.4.99.‑)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/17);  total (37/17)

GGCTACAATCTGGTGTTCGTCAATAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATTAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCACACTTGCTATTTTCAAGCTGTTAGAACGATCAAAATCCTGTATAATCTAGGCAATTTTTTGTTTAAATAATGCATTGTGAGATCCCTTAATGCTTGAAACATC  >  1/1054502‑1054932                                                                                                                                                                                                       |                                                                                                                                                                                                                                         ggCTACAATCTGGTGTTCGTCAATAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTtg                                                                                                                                                                                      >  1:757478/1‑251 (MQ=255)     aCAATCTGGTGTTCGTCAATAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGc                                                                                                                                                                                   >  1:408681/1‑250 (MQ=255)          cTGGTGTTCGTCAATAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGaaa                                                                                                                                                                                                              <  1:405315/218‑1 (MQ=255)                       aTAAAGTGCCTTGCAATAGTCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGAtttt                                                                                                                                                                >  1:144755/1‑251 (MQ=255)                       aTAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGGTCTTGTGCCCCTTTTTTCGCCGAtttt                                                                                                                                                                >  1:862168/1‑251 (MQ=255)                               ccTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCCAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGaaa                                                                                                                                                        >  1:481359/1‑250 (MQ=255)                                  tGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGGTCTTGTGCCCCTTTTTTCGCCGATTTTTATAg                                                                                                                                                           >  1:114481/1‑245 (MQ=255)                                  tGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGt                                                                                                                                                     >  1:60039/1‑251 (MQ=255)                                                           tGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAgg                                                                                                                                                                                             >  1:815469/1‑186 (MQ=255)                                                           tGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTtg                                                                                                                                                                                      <  1:406121/193‑1 (MQ=255)                                                                 gCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCAGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAttctt                                                                                                                      >  1:449800/1‑251 (MQ=255)                                                                     gAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAt                                                                                                                          >  1:29500/1‑243 (MQ=255)                                                                     gAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAAGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCaaa                                                                                                                  >  1:162995/1‑251 (MQ=255)                                                                             gTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTcac                                                                                                           >  1:681469/1‑250 (MQ=255)                                                                               tcttcGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTcaccg                                                                                                         >  1:858421/1‑249 (MQ=255)                                                                                cttcGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTcaccgca                                                                                                       >  1:339160/1‑248 (MQ=255)                                                                                     gCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTtg                                                                                                                                                                                      >  1:247759/1‑167 (MQ=255)                                                                                        tatCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTAt                                                                                               <  1:452668/251‑1 (MQ=255)                                                                                             cTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCa                                                                                          >  1:653629/1‑251 (MQ=255)                                                                                                        ccAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaat                                                                               <  1:593525‑M1/251‑4 (MQ=255)                                                                                                        ccAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaat                                                                               <  1:269542‑M1/251‑4 (MQ=255)                                                                                                          aaaaaTAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcc                                                                             <  1:164557‑M1/251‑6 (MQ=255)                                                                                                           aaaaTAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAttcttc                                                                                                                     >  1:361135/1‑210 (MQ=255)                                                                                                            aaataaatTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTa                                                                                                >  1:889852/1‑230 (MQ=255)                                                                                                                   ttGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatc                                                                    <  1:483043‑M1/251‑15 (MQ=255)                                                                                                                    tGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatct                                                                   <  1:148538‑M1/251‑16 (MQ=255)                                                                                                                          aaTATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaa                                                             >  1:376611‑M1/1‑230 (MQ=255)                                                                                                                              tGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAt                                                                                                                          <  1:768040/186‑1 (MQ=255)                                                                                                                                gTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaat                                                                               <  1:368494‑M1/227‑4 (MQ=255)                                                                                                                                   gTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtt                                                    >  1:485400‑M1/1‑221 (MQ=255)                                                                                                                                         cgTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttttttaaata                                              >  1:798363‑M1/1‑215 (MQ=255)                                                                                                                                                   cAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtg                                    <  1:97078‑M1/251‑47 (MQ=255)                                                                                                                                                   cAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcat                                        <  1:66186‑M1/247‑43 (MQ=255)                                                                                                                                                       gTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCCCTTGCt                                                                                                 >  1:130105/1‑186 (MQ=255)                                                                                                                                                        tgctgcGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagat                                <  1:228714‑M1/250‑51 (MQ=255)                                                                                                                                                                 aCTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATTTTGTGCCCCTTTTTTCGCCGCTTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGCTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatg                       >  1:178034‑M1/1‑191 (MQ=255)                                                                                                                                                                 aCTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGGTTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtat                                                                        >  1:801315‑M1/1‑191 (MQ=255)                                                                                                                                                                  cTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAg                                                                                                                                                                                              >  1:292111/1‑82 (MQ=255)                                                                                                                                                                        ggATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaa                >  1:250902‑M1/1‑184 (MQ=255)                                                                                                                                                                        ggATGATATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgaga                                 <  1:420310‑M1/233‑50 (MQ=255)                                                                                                                                                                          atgatATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacat             >  1:696314‑M1/1‑182 (MQ=255)                                                                                                                                                                           tgatATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAt                                                                                                                          >  1:563234/1‑141 (MQ=255)                                                                                                                                                                            gatATGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatg                       >  1:694877‑M1/1‑180 (MQ=255)                                                                                                                                                                               atGCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacatcacaa        >  1:403880‑M1/1‑177 (MQ=255)                                                                                                                                                                                 gCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacatcacaaa       >  1:881119‑M1/1‑175 (MQ=255)                                                                                                                                                                                 gCAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaac               >  1:336355‑M1/1‑175 (MQ=255)                                                                                                                                                                                  cAGTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacatcacaaac      <  1:118732‑M1/250‑77 (MQ=255)                                                                                                                                                                                    gaatgAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAATGGATCTTGTGCCCCTTTTTTTGCCGATTTTTATAGAATCGGTATCTTGAAAAGAAAACTGTTATAAATTTTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaattctgtataattt                                                                   >  1:549650‑M1/3‑172 (MQ=255)                                                                                                                                                                                    gTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacatcacaaacaat   >  1:524373‑M1/1‑172 (MQ=255)                                                                                                                                                                                    gTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggca                                                              <  1:258933‑M1/192‑21 (MQ=255)                                                                                                                                                                                    gTATGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatct                                                                   >  1:196339‑M1/1‑172 (MQ=255)                                                                                                                                                                                      aTGAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatctaggcaattttttgtttaaataatgcattgtgagatcccttaatgcttgaaacatcacaaacaatc  >  1:88677‑M1/1‑170 (MQ=255)                                                                                                                                                                                        gAAGAGGGCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTACTTTCAAGCTGTTAaatcctgtataa                                                                      >  1:92080‑M1/1‑168 (MQ=255)                                                                                                                                                                                               gCTATGATGAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAAt                                                                                                                          <  1:845522/121‑1 (MQ=255)                                                                                                                                                                                                  atgatgAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCGCACTTGCTATTTTCAAGCTGTTAaatcctgtataatct                                                                   >  1:694215‑M1/1‑158 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GGCTACAATCTGGTGTTCGTCAATAAAGTGCCTTGCAATAATCGTGCCACCATCGGCGTGATTAGCCTGAATAACAGTTCTTCGGCATATCGCTCCAGTTTCACCAAAAATAAATTGGAAAAATATGGTTGTACCGCGTTAAACAGCAAGGTGCTGCGAGACTTTGTGGATGATATGCAGTATGAAGAGGGCTATGATTAAGCCGGAATGGACGAATTAATCCGAAAAGTGGCGGGCTAGAAGGGATCTTGTGCCCCTTTTTTCGCCGATTTTTATAGAATCGTTATCTTGAAAAGAAAACCGTTATAAATTCTTCAAATTTTCACCACACTTGCTATTTTCAAGCTGTTAGAACGATCAAAATCCTGTATAATCTAGGCAATTTTTTGTTTAAATAATGCATTGTGAGATCCCTTAATGCTTGAAACATC  >  1/1054502‑1054932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑