Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,051,740	C→T	intergenic (+9/+110)	unknown → / ← unknown	Glycogen phosphorylase (EC 2.4.1.1)/DedA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,051,740	0	C	T	100.0%	192.7 / NA	71	intergenic (+9/+110)	unknown/unknown	Glycogen phosphorylase (EC 2.4.1.1)/DedA protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/30);  total (41/30)

GTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATCAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGATTACTGGCAC  >  1/1051509‑1051983                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCat                                                                                                                                                                                                                                  >  1:557317/1‑251 (MQ=255)           ctcCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCgg                                                                                                                                                                                                                        >  1:454014/1‑251 (MQ=255)             ccAACATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCgggg                                                                                                                                                                                                                      >  1:676647/1‑251 (MQ=255)                      cGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttta                                                                                                                                                                                                             >  1:263955/1‑251 (MQ=255)                        aCTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTg                                                                                                                                                                                                                             >  1:868172/1‑233 (MQ=255)                           attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATGTGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                                                        >  1:91690/1‑251 (MQ=255)                           attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                                                        >  1:683405/1‑251 (MQ=255)                           attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                                                        >  1:212534/1‑251 (MQ=255)                            ttatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                                               <  1:793297/227‑1 (MQ=255)                            ttatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGt                                                                                                                                                                                                                <  1:737622/242‑1 (MQ=255)                              atCAGGCATTCGCCGATTTCCGCAGTTATGCGGATATGCAAAAAGCGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGaa                                                                                                                                                                                                     >  1:425286/1‑251 (MQ=255)                                             aTTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAAt                                                                                                                                                                                      <  1:176769/251‑1 (MQ=255)                                                 ccGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttt                                                                                                                                                                                                              <  1:387533/223‑1 (MQ=255)                                                    cAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAgt                                                                                                                                                                               >  1:721819/1‑251 (MQ=255)                                                      gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                                               >  1:519518/1‑201 (MQ=255)                                                      gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                                                         <  1:835485/223‑1 (MQ=255)                                                      gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                                                         <  1:473906/223‑1 (MQ=255)                                                      gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAgtgt                                                                                                                                                                             >  1:762254/1‑251 (MQ=255)                                                       ttATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttta                                                                                                                                                                                                             <  1:774224/218‑1 (MQ=255)                                                        tATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGaaa                                                                                                                                                                                                    >  1:468482/1‑226 (MQ=255)                                                              gATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAg                                                                                                                                                                     >  1:435020/1‑251 (MQ=255)                                                               atatGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAg                                                                                                                                                                     <  1:523238/250‑1 (MQ=255)                                                                 atGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATc                                                                                                                                                                                                                                              >  1:32333/1‑175 (MQ=255)                                                                  tGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                                                         >  1:235413/1‑211 (MQ=255)                                                                                     gaAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAgacgac                                                                                                                                               >  1:384699/1‑250 (MQ=255)                                                                                     gaAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAgacgac                                                                                                                                               >  1:279613/1‑250 (MQ=255)                                                                                                       tGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                                               >  1:713082/1‑152 (MQ=255)                                                                                                            ggATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttg                                                                                                                       >  1:750052/1‑251 (MQ=255)                                                                                                              aTTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTA‑TTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                                                    <  1:670301/251‑1 (MQ=255)                                                                                                               ttGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                                                    <  1:593306/251‑1 (MQ=255)                                                                                                                 gAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTg                                                                                                                                                                                                                             <  1:303050/144‑1 (MQ=255)                                                                                                                        ccATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAAtt                                                                                                                                                                                     >  1:863274/1‑177 (MQ=255)                                                                                                                          aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgt                                                                                                                      <  1:256778/238‑1 (MQ=255)                                                                                                                          aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTta                                                                                                         >  1:724147/1‑251 (MQ=255)                                                                                                                          aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTta                                                                                                         >  1:553893/1‑251 (MQ=255)                                                                                                                             cAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAc                                                                                                      <  1:728144/251‑1 (MQ=255)                                                                                                                             cAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAc                                                                                                      <  1:110626/251‑1 (MQ=255)                                                                                                                              aaaaCATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                                                     <  1:489032/251‑1 (MQ=255)                                                                                                                              aaaaCATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                                                     <  1:272814/251‑1 (MQ=255)                                                                                                                                   aTCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                                                     <  1:794927/246‑1 (MQ=255)                                                                                                                                        cAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGc                                                                                           <  1:326026/251‑1 (MQ=255)                                                                                                                                               ggCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                                                    <  1:599083/219‑1 (MQ=255)                                                                                                                                               ggCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAat                                                                                    >  1:743450/1‑251 (MQ=255)                                                                                                                                                 cTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAatat                                                                                  >  1:381750/1‑251 (MQ=255)                                                                                                                                                   aTTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                                                     <  1:389219/230‑1 (MQ=255)                                                                                                                                                    tttCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTc                                                                                                >  1:78189/1‑234 (MQ=255)                                                                                                                                                    tttCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGa                                                                                >  1:835146/1‑250 (MQ=255)                                                                                                                                                                     cGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGaa                                                              >  1:224080/1‑251 (MQ=255)                                                                                                                                                                      gATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAat                                                             >  1:29129/1‑251 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAg                                                                                                                                    >  1:666730/1‑179 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTc                                                                                                <  1:767085/215‑1 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAAc                                                                         <  1:452786/238‑1 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                                                            >  1:41387/1‑251 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                                                            >  1:275692/1‑251 (MQ=255)                                                                                                                                                                       aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                                                            >  1:612886/1‑251 (MQ=255)                                                                                                                                                                         tAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAAtata                                                          >  1:356972/1‑251 (MQ=255)                                                                                                                                                                             gAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATaaa                                                        <  1:379122/249‑1 (MQ=255)                                                                                                                                                                               atatGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCAc                                                    <  1:667065/251‑1 (MQ=255)                                                                                                                                                                                            atCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCtttt                                       >  1:900540/1‑251 (MQ=255)                                                                                                                                                                                                ggAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAatat                                                           >  1:117142/1‑227 (MQ=255)                                                                                                                                                                                                ggAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATaaa                                                        <  1:52199/230‑1 (MQ=255)                                                                                                                                                                                                   atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt                                >  1:761446/1‑251 (MQ=255)                                                                                                                                                                                                   atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt                                >  1:397986/1‑251 (MQ=255)                                                                                                                                                                                                   atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt                                >  1:284205/1‑251 (MQ=255)                                                                                                                                                                                                           ccGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                                               >  1:521213/1‑52 (MQ=255)                                                                                                                                                                                                           ccGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAc                                                                                                      >  1:479231/1‑173 (MQ=255)                                                                                                                                                                                                                         aaaaTAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGAtt          <  1:76426/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaaTAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGAtt          <  1:468032/251‑1 (MQ=255)                                                                                                                                                                                                                           aataatCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGATTAc        <  1:680453/251‑1 (MQ=255)                                                                                                                                                                                                                              aatCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGATTACTgg     <  1:732802/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGATTACTGGCAc  <  1:74581/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATCAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTTGTTCGTGCAACCAATCCAGATTACTGGCAC  >  1/1051509‑1051983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑