Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,044,471	G→A	G106D (GGT→GAT)	unknown →	Limit dextrin alpha‑1,6‑maltotetraose‑hydrolase (EC 3.2.1.196)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,044,471	0	G	A	100.0%	189.8 / NA	67	G106D (GGT→GAT)	unknown	Limit dextrin alpha‑1,6‑maltotetraose‑hydrolase (EC 3.2.1.196)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/30);  total (37/30)

GATCACGAACTTCGCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGAGGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGGTTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGCCGGTT  >  1/1044226‑1044695                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTaa                                                                                                                                                                                                                             >  1:667133/1‑251 (MQ=255) gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTaa                                                                                                                                                                                                                             >  1:779806/1‑251 (MQ=255)   tCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGc                                                                                                                                                                                                                           <  1:122765/251‑1 (MQ=255)            tctcccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGaa                                                                                                                                                                                                                  >  1:589820/4‑251 (MQ=255)               cccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACg                                                                                                                                                                                                               >  1:459066/1‑251 (MQ=255)               cccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACg                                                                                                                                                                                                               >  1:326599/1‑251 (MQ=255)                                gcgcAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTttgt                                                                                                                                                                                               <  1:50260/250‑1 (MQ=255)                                       aGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATACTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGcga                                                                                                                                                                                        >  1:422975/1‑250 (MQ=255)                                                            aaGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTa                                                                                                                                                                   <  1:105822/250‑1 (MQ=255)                                                                tgagCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCAc                                                                                                                                                              <  1:808480/250‑1 (MQ=255)                                                                 gagCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGcc                                                                                                                                                                        >  1:548499/1‑240 (MQ=255)                                                                  agCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACATGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCAcc                                                                                                                                                             <  1:712776/250‑1 (MQ=255)                                                                    cGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCg                                                                                                                                                            <  1:8439/249‑1 (MQ=255)                                                                      ggAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTCGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGaaa                                                                                                                                                         <  1:419699/250‑1 (MQ=255)                                                                         aTCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCgg                                                                                                                                                     >  1:25617/1‑251 (MQ=255)                                                                                 tCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGtt                                                                                                                                             <  1:809039/251‑1 (MQ=255)                                                                                    aCATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGAt                                                                                                                                          <  1:25161/251‑1 (MQ=255)                                                                                    aCATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGAt                                                                                                                                          <  1:861158/251‑1 (MQ=255)                                                                                        ggTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTg                                                                                                                                      >  1:374541/1‑251 (MQ=255)                                                                                            aaTTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCAtt                                                                                                                                  <  1:679399/251‑1 (MQ=255)                                                                                                        gtgtGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGa                                                                                                                                                           >  1:141491/1‑214 (MQ=255)                                                                                                        gtgtGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCagag                                                                                                                      >  1:632939/1‑251 (MQ=255)                                                                                                        gtgtGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCagag                                                                                                                      >  1:80852/1‑251 (MQ=255)                                                                                                              cATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACaa                                                                                                                <  1:21112/251‑1 (MQ=255)                                                                                                                              gggTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGcc                                                                                                 >  1:770201/1‑250 (MQ=255)                                                                                                                                    cAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGAcc                                                                                          <  1:88290/251‑1 (MQ=255)                                                                                                                                    cAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGAc                                                                                           <  1:437651/250‑1 (MQ=255)                                                                                                                                            aCCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGTATTTAAGCTCGCCTGAAACGCAAGCGGGGATTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCAt                                                                                  >  1:38452/1‑251 (MQ=255)                                                                                                                                            aCCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCAt                                                                                  >  1:684939/1‑251 (MQ=255)                                                                                                                                               cAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATtgt                                                                               >  1:130832/1‑251 (MQ=255)                                                                                                                                                     ggCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtga                                                                         >  1:755305/1‑251 (MQ=255)                                                                                                                                                     ggCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtga                                                                         >  1:102674/1‑251 (MQ=255)                                                                                                                                                      gCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGAt                                                                                                                                          >  1:322676/1‑185 (MQ=255)                                                                                                                                                       cTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        <  1:877716/250‑1 (MQ=255)                                                                                                                                                       cTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        <  1:612408/250‑1 (MQ=255)                                                                                                                                                       cTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        <  1:671561/250‑1 (MQ=255)                                                                                                                                                       cTTCAGAATCACATCAAAAAATGACCGCACGTTGACAGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        <  1:387253/250‑1 (MQ=255)                                                                                                                                                            gAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgagt                                                                       >  1:238887/1‑246 (MQ=255)                                                                                                                                                                    tCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        >  1:129196/1‑237 (MQ=255)                                                                                                                                                                    tCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAgg                                                          >  1:387658/1‑251 (MQ=255)                                                                                                                                                                       aaaaaTGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGc                                                                                                                                                                         <  1:821765/137‑1 (MQ=255)                                                                                                                                                                       aaaaaTGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATAc                                                                                                    >  1:576546/1‑206 (MQ=255)                                                                                                                                                                       aaaaaTGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGttt                                                       >  1:406737/1‑251 (MQ=255)                                                                                                                                                                          aaTGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTAc                                                    <  1:538097/251‑1 (MQ=255)                                                                                                                                                                          aaTGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTAc                                                    <  1:480175/251‑1 (MQ=255)                                                                                                                                                                            tGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCa                                                  >  1:95923/1‑251 (MQ=255)                                                                                                                                                                                  cACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGCTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTgtgt                                                                             >  1:658945/1‑218 (MQ=255)                                                                                                                                                                                    cTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAt                                                                           >  1:566407/1‑218 (MQ=255)                                                                                                                                                                                    cTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAgg                                                          >  1:289371/1‑235 (MQ=255)                                                                                                                                                                                      ttGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAAc                                        <  1:475780/251‑1 (MQ=255)                                                                                                                                                                                              aaaTCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCATGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTAc                                <  1:538826/251‑1 (MQ=255)                                                                                                                                                                                                 tCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCag                             <  1:348577/251‑1 (MQ=255)                                                                                                                                                                                                        aaaTTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGAc                         <  1:745331/248‑1 (MQ=255)                                                                                                                                                                                                         aaTTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATa                     >  1:571584/1‑251 (MQ=255)                                                                                                                                                                                                          aTTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATAc                    <  1:46183/251‑1 (MQ=255)                                                                                                                                                                                                          aTTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATAc                    <  1:189214/251‑1 (MQ=255)                                                                                                                                                                                                                      cccTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgagt                                                                       <  1:860870/188‑1 (MQ=255)                                                                                                                                                                                                                      cccTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGTGACAATACGCGGCACCTATg        >  1:378653/1‑251 (MQ=255)                                                                                                                                                                                                                      cccTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATg        >  1:127447/1‑251 (MQ=255)                                                                                                                                                                                                                      cccTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATg        >  1:92192/1‑251 (MQ=255)                                                                                                                                                                                                                        cTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGc       >  1:635584/1‑250 (MQ=255)                                                                                                                                                                                                                            tGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTAcc                                                   <  1:392229/202‑1 (MQ=255)                                                                                                                                                                                                                            tGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGCCGGtt  >  1:163696/1‑251 (MQ=255)                                                                                                                                                                                                                            tGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGCCGGtt  >  1:419054/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATtgtg                                                                              >  1:418356/1‑168 (MQ=255)                                                                                                                                                                                                                                      gtggtgGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTAtgag                                                                        >  1:199675/1‑171 (MQ=255)                                                                                                                                                                                                                                      gtggtgGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGCCgg    <  1:254464/239‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 GATCACGAACTTCGCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGAGGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGGTTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTATGCACCGAAAGCGGTGGTGGTTGATCGTGCATTTGACTGGCAGAGTGACAAAGCGCCGAATACGCCCTGGACCGAAACCATTGTGTATGAGTTGAGTGTGAAAGGTTTTACCAAATTACAAACGGAATTACCAGAGACAATACGCGGCACCTATGCCGGTT  >  1/1044226‑1044695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑