Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,042,446	C→T	R162C (CGT→TGT)	unknown →	1,4‑alpha‑glucan (glycogen) branching enzyme, GH‑13‑type (EC 2.4.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,042,446	0	C	T	100.0%	209.7 / NA	73	R162C (CGT→TGT)	unknown	1,4‑alpha‑glucan (glycogen) branching enzyme, GH‑13‑type (EC 2.4.1.18)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/31);  total (42/31)

TGCTTATCAACTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTCGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTAAAGCTAACCAATGGGAT  >  1/1042202‑1042682                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             tGCTTATCAACTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGcc                                                                                                                                                                                                                                        <  1:816551/251‑1 (MQ=255)      aTCAACTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATcc                                                                                                                                                                                                                                    <  1:542679/250‑1 (MQ=255)      aTCAACTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCa                                                                                                                                                                                                                                   >  1:144615/1‑251 (MQ=255)         aaCTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATg                                                                                                                                                                                                                                >  1:878907/1‑251 (MQ=255)          aCTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATgc                                                                                                                                                                                                                               <  1:325395/251‑1 (MQ=255)          aCTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATgc                                                                                                                                                                                                                               <  1:292791/251‑1 (MQ=255)           cTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATc                                                                                                                                                                                                                                     >  1:73717/1‑244 (MQ=255)                ggTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTa                                                                                                                                                                                                                          >  1:832286/1‑250 (MQ=255)                  tgtATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       <  1:289188/251‑1 (MQ=255)                   gtATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       >  1:121652/1‑250 (MQ=255)                   gtATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       >  1:550136/1‑250 (MQ=255)                   gtATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       >  1:846271/1‑250 (MQ=255)                    tATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACAccc                                                                                                                                                                                                                     <  1:254003/251‑1 (MQ=255)                        ggggTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATgcgc                                                                                                                                                                                                                             <  1:609132/239‑1 (MQ=255)                                gagTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGcc                                                                                                                                                                                                                                        <  1:284745/220‑1 (MQ=255)                                gagTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCgt                                                                                                                                                                                                         >  1:100314/1‑251 (MQ=255)                                  gTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCgtgt                                                                                                                                                                                                       >  1:478645/1‑251 (MQ=255)                                          ttattGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGcc                                                                                                                                                                                                                                        <  1:176849/210‑1 (MQ=255)                                          ttattGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATcc                                                                                                                                                                                                                                    >  1:625134/1‑214 (MQ=255)                                          ttattGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCa                                                                                                                                                                                                                 <  1:513867/233‑1 (MQ=255)                                                  gACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       <  1:727789/219‑1 (MQ=255)                                                          ccGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGt                                                                                                                                                                                 >  1:23381/1‑249 (MQ=255)                                                            gTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTggg                                                                                                                                                                                                    >  1:553930/1‑228 (MQ=255)                                                                       atgatTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCaa                                                                                                                                                                    >  1:573726/1‑249 (MQ=255)                                                                         gatTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGTGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGc                                                                                                                                                                      >  1:614493/1‑245 (MQ=255)                                                                              aTGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATaaa                                                                                                                                                           <  1:800982/251‑1 (MQ=255)                                                                              aTGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATaaa                                                                                                                                                           <  1:782800/251‑1 (MQ=255)                                                                               tGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTc                                                                                                                                                                                            >  1:696784/1‑217 (MQ=255)                                                                                gATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAAtt                                                                                                                                                         >  1:27894/1‑251 (MQ=255)                                                                                gATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGGGGGAATTGGTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAAtt                                                                                                                                                         >  1:324098/1‑251 (MQ=255)                                                                                 aTTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAAtt                                                                                                                                                         <  1:734452/250‑1 (MQ=255)                                                                                 aTTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAAtt                                                                                                                                                         <  1:433666/250‑1 (MQ=255)                                                                                      gATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAAt                                                                                                                                                    <  1:11390/250‑1 (MQ=255)                                                                                          aaTGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATc                                                                                                                                               <  1:225244/251‑1 (MQ=255)                                                                                            tGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGa                                                                                                                                             >  1:331772/1‑251 (MQ=255)                                                                                                             ttCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTc                                                                                                                             <  1:79398/250‑1 (MQ=255)                                                                                                                 tttttACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCtt                                                                                                                        >  1:178424/1‑251 (MQ=255)                                                                                                                  ttttACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAAc                                                                                                                                >  1:513653/1‑242 (MQ=255)                                                                                                                       cGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGc                                                                                                                  <  1:524780/251‑1 (MQ=255)                                                                                                                           cGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTacac                                                                                                                                                                                                                       >  1:457935/1‑146 (MQ=255)                                                                                                                           cGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGt                                                                                                                                                                                 >  1:282790/1‑184 (MQ=255)                                                                                                                            gTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGAc                                                                                                              >  1:777399/1‑250 (MQ=255)                                                                                                                              aTGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATaa                                                                                                                                                            >  1:34746/1‑202 (MQ=255)                                                                                                                                 aaGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTAt                                                                                                        >  1:199943/1‑251 (MQ=255)                                                                                                                                 aaGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTAt                                                                                                        >  1:854560/1‑251 (MQ=255)                                                                                                                                  aGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATg                                                                                                       >  1:491782/1‑251 (MQ=255)                                                                                                                                   gTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGcc                                                                                                                                                                                                                                        >  1:521167/1‑121 (MQ=255)                                                                                                                                   gTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATaaa                                                                                                                                                           <  1:709114/198‑1 (MQ=255)                                                                                                                                   gTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTg                                                                                                                                          >  1:578947/1‑215 (MQ=255)                                                                                                                                                 tttCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGt                                                                                        <  1:631271/251‑1 (MQ=255)                                                                                                                                                    cATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCGAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAg                                                                                         >  1:527049/1‑247 (MQ=255)                                                                                                                                                        gAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGt                                                                                        <  1:609504/244‑1 (MQ=255)                                                                                                                                                          aTGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCg                                                                               >  1:500627/1‑251 (MQ=255)                                                                                                                                                              gACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATa                                                                            >  1:661058/1‑250 (MQ=255)                                                                                                                                                               aCGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATAc                                                                           <  1:891359/250‑1 (MQ=255)                                                                                                                                                                  gtgtCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAAtt                                                                                                                                                         <  1:459647/169‑1 (MQ=255)                                                                                                                                                                   tgtCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTc                                                                                                                             >  1:349318/1‑196 (MQ=255)                                                                                                                                                                    gtCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGt                                                                      >  1:260258/1‑250 (MQ=255)                                                                                                                                                                     tctcAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCaa                                                                                                                                 <  1:704371/190‑1 (MQ=255)                                                                                                                                                                            cGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATc                                                              <  1:887048/250‑1 (MQ=255)                                                                                                                                                                                            tgGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAgtgt                                             <  1:730026/251‑1 (MQ=255)                                                                                                                                                                                            tgGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAgtgt                                             <  1:545041/251‑1 (MQ=255)                                                                                                                                                                                                  ccAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGc                                        >  1:741174/1‑250 (MQ=255)                                                                                                                                                                                                        gCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAgtgt                                             >  1:786481/1‑239 (MQ=255)                                                                                                                                                                                                                ggTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGt                                                                                   <  1:874377/193‑1 (MQ=255)                                                                                                                                                                                                                ggTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGcc                          >  1:128074/1‑250 (MQ=255)                                                                                                                                                                                                                  tttCTGTGGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCcgt                       >  1:828389/1‑251 (MQ=255)                                                                                                                                                                                                                       gtgGTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTaa                  >  1:803748/1‑251 (MQ=255)                                                                                                                                                                                                                          gTAGGCGATTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTAAAg                <  1:51239/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTTTTAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGc                                                                                                      >  1:476395/1‑157 (MQ=255)                                                                                                                                                                                                                                     tAACTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTAAAGc               <  1:857831/240‑1 (MQ=255)                                                                                                                                                                                                                                        cTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTc                                                                                                                             >  1:649051/1‑127 (MQ=255)                                                                                                                                                                                                                                        cTATTGGGACGGTTGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTAAAGCTAACCAATGGGAt  >  1:39149/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             TGCTTATCAACTACAGGTGTATTGGGGTAAAGAGTCACAAATTATTGAAGACCCGTACCGTTATCACCCGATGATTAATGATTTGGATGAATGGTTGTTGGCGGAAGGTTCGTTTTTACGTCCGTATGAAGTATTAGGCGCGCATTTCATTGAATGTGACGGTGTCTCAGGCGTGAATTTCCGTTTGTGGGCGCCAAATGCCAAACGGGTTTCTGTGGTAGGCGATTTTAACTATTGGGACGGTCGTCGCCATCCAATGCGCTTACACCCGGCAAGCGGCGTGTGGGAATTGTTCCTGCCGAAAGTCTCTTTGGGGCAACTGTATAAATTTGAATTAATCGATTGCCATGGCAACCTCCGCTTAAAAGCCGACCCTTATGCCTTCAGTTCTCAGTTACGTCCCGATACCGCGTCACAAATCAGTCCGTTGCCGAGTGTCGTGCCGATGACGGAAGCCCGTCGTAAAGCTAACCAATGGGAT  >  1/1042202‑1042682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑