Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,041,002	A→G	E44G (GAA→GGA)	unknown →	UPF0260 protein YcgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,041,002	0	A	G	100.0%	221.8 / NA	77	E44G (GAA→GGA)	unknown	UPF0260 protein YcgN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/37);  total (40/37)

TGGTATTCAACCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGAACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAGCGTGAAAAAAGCCGATATT  >  1/1040760‑1041243                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  tGGTATTCAACCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGt                                                                                                                                                                                                                                           <  1:208670/251‑1 (MQ=255)       tCAACCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTaca                                                                                                                                                                                                                                     <  1:616397/251‑1 (MQ=255)        cAACCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTacac                                                                                                                                                                                                                                    <  1:218161/251‑1 (MQ=255)         aaCCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACAcg                                                                                                                                                                                                                                   <  1:667932/251‑1 (MQ=255)              gACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACa                                                                                                                                                                                                                                                <  1:136496/233‑1 (MQ=255)              gACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCAt                                                                                                                                                                                                                              >  1:678283/1‑251 (MQ=255)                        ttGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAAt                                                                                                                                                                                                                    <  1:89713/251‑1 (MQ=255)                         tGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATc                                                                                                                                                                                                                   <  1:115924/251‑1 (MQ=255)                                  gATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTgttggagt                                                                                                                                                                                                          >  1:684268/1‑251 (MQ=255)                                        aaaCTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGaaa                                                                                                                                                                                                    <  1:51993/251‑1 (MQ=255)                                                tCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTAt                                                                                                                                                                                                                                         <  1:699268/206‑1 (MQ=255)                                                 cGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAAt                                                                                                                                                                                                                    >  1:199987/1‑226 (MQ=255)                                                   cATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTg                                                                                                                                                                                          >  1:570746/1‑250 (MQ=255)                                                       aaGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGt                                                                                                                                                                                      >  1:533535/1‑250 (MQ=255)                                                            cGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACAGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAatat                                                                                                                                                                                <  1:676595/251‑1 (MQ=255)                                                               tCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTc                                                                                                                                                                              <  1:650199/250‑1 (MQ=255)                                                                 gTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACAcg                                                                                                                                                                                                                                   <  1:289732/195‑1 (MQ=255)                                                                   ttttCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGc                                                                                                                                                                         <  1:98658/251‑1 (MQ=255)                                                                            tGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAAt                                                                                                                                                                <  1:78352/251‑1 (MQ=255)                                                                             gAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAAtt                                                                                                                                                               >  1:852870/1‑251 (MQ=255)                                                                               aTTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTgg                                                                                                                                                             >  1:663837/1‑251 (MQ=255)                                                                                           cTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTc                                                                                                                                                                              >  1:529415/1‑222 (MQ=255)                                                                                            ttttCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCAc                                                                                                                                                <  1:539210/251‑1 (MQ=255)                                                                                             tttCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCAc                                                                                                                                                                            <  1:477703/222‑1 (MQ=255)                                                                                                cTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAg                                                                                                                                            >  1:869181/1‑251 (MQ=255)                                                                                                 tttATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGc                                                                                                                                           <  1:785293/251‑1 (MQ=255)                                                                                                 tttATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACAAGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGc                                                                                                                                           <  1:419426/251‑1 (MQ=255)                                                                                                               cTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTTACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAAc                                                                                                                             <  1:257411/251‑1 (MQ=255)                                                                                                                      aTTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCgg                                                                                                                      >  1:721313/1‑251 (MQ=255)                                                                                                                      aTTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCgg                                                                                                                      >  1:327819/1‑251 (MQ=255)                                                                                                                                         cTACACAAAACCTTACTTGACATGAACGATGCGGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGc                                                                                                   <  1:484734/251‑1 (MQ=255)                                                                                                                                           acacAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCa                                                                                                 >  1:452190/1‑251 (MQ=255)                                                                                                                                             acaAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCAcc                                                                                               <  1:626470/251‑1 (MQ=255)                                                                                                                                                aaaCCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCAc                                                                                                >  1:671507/1‑247 (MQ=255)                                                                                                                                                  aCCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGAt                                                                                                              <  1:363789/231‑1 (MQ=255)                                                                                                                                                   ccTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGACGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCtgtg                                                                                           >  1:449622/1‑249 (MQ=255)                                                                                                                                                           gACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGAc                                                                                                                                                     >  1:222562/1‑183 (MQ=255)                                                                                                                                                           gACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGttt                                                                                 >  1:43053/1‑251 (MQ=255)                                                                                                                                                           gACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGttt                                                                                 >  1:50895/1‑251 (MQ=255)                                                                                                                                                           gACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGttt                                                                                 >  1:641503/1‑251 (MQ=255)                                                                                                                                                                gAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTttatta                                                                             >  1:245249/1‑250 (MQ=255)                                                                                                                                                                gAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTttatta                                                                             >  1:497674/1‑250 (MQ=255)                                                                                                                                                                 aaCGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACa                                                                           <  1:233395/251‑1 (MQ=255)                                                                                                                                                                     aTGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGaa                                                                       >  1:832763/1‑251 (MQ=255)                                                                                                                                                                            gTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTAc                                                                            >  1:544483/1‑239 (MQ=255)                                                                                                                                                                                        aTGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACa                                                                           <  1:369777/228‑1 (MQ=255)                                                                                                                                                                                        aTGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGCCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCAt                                                    >  1:543085/1‑251 (MQ=255)                                                                                                                                                                                         tgtgACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAAc                                                                >  1:334406/1‑238 (MQ=255)                                                                                                                                                                                         tgtgACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCg                                                              <  1:312036/240‑1 (MQ=255)                                                                                                                                                                                         tgtgACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATg                                                   <  1:110924/251‑1 (MQ=255)                                                                                                                                                                                                ggTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCg                                            >  1:607402/1‑251 (MQ=255)                                                                                                                                                                                                ggTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCg                                            >  1:391765/1‑251 (MQ=255)                                                                                                                                                                                                 gtgtGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCg                                            >  1:348893/1‑250 (MQ=255)                                                                                                                                                                                                 gtgtGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCg                                            >  1:843732/1‑250 (MQ=255)                                                                                                                                                                                                   gtgGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCtt                                         >  1:857290/1‑251 (MQ=255)                                                                                                                                                                                                        aaaTGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAg                                    <  1:395643/251‑1 (MQ=255)                                                                                                                                                                                                             ttGCTATCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAAcc                                                               <  1:421247/219‑1 (MQ=255)                                                                                                                                                                                                                  aTCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATcc                           >  1:851917/1‑250 (MQ=255)                                                                                                                                                                                                                  aTCGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGATTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATcc                           >  1:432558/1‑250 (MQ=255)                                                                                                                                                                                                                    cGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGAc                                                                                                                                                     >  1:66719/1‑126 (MQ=255)                                                                                                                                                                                                                    cGTAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTAccc                                                                                                     >  1:441204/1‑174 (MQ=255)                                                                                                                                                                                                                      tAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCgg                                                                                                                      <  1:677288/155‑1 (MQ=255)                                                                                                                                                                                                                      tAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACa                                                                           >  1:77399/1‑198 (MQ=255)                                                                                                                                                                                                                      tAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGAt                       <  1:791086/250‑1 (MQ=255)                                                                                                                                                                                                                      tAAATATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGAt                       <  1:693057/250‑1 (MQ=255)                                                                                                                                                                                                                       aaaTATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAACCTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAg                     <  1:223874/251‑1 (MQ=255)                                                                                                                                                                                                                       aaaTATATTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAg                     <  1:441671/251‑1 (MQ=255)                                                                                                                                                                                                                           atatTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGt                                                                                   <  1:207824/185‑1 (MQ=255)                                                                                                                                                                                                                           atatTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAg                     >  1:733786/1‑247 (MQ=255)                                                                                                                                                                                                                             atTGACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGAt                       <  1:131110/243‑1 (MQ=255)                                                                                                                                                                                                                                gACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACa                                                                           >  1:215538/1‑188 (MQ=255)                                                                                                                                                                                                                                gACGGCTATGGCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAGCGTGaaaaa            >  1:798338/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggCAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAGCGTGAAAAAAGCCGatat   >  1:482720/1‑251 (MQ=255)                                                                                                                                                                                                                                           cAAATATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAGCGTGAAAAAAGCCGATAtt  <  1:821744/250‑1 (MQ=255)                                                                                                                                                                                                                                             aaTATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAAc                                                                                                                             >  1:278181/1‑125 (MQ=255)                                                                                                                                                                                                                                             aaTATGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTttatta                                                                             >  1:786346/1‑173 (MQ=255)                                                                                                                                                                                                                                              atatGGACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATc                                      <  1:605240/211‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  TGGTATTCAACCTGACAGTGAGTTTGAAAGAAGGATTTTAAACTCAATCGCATAAAGTGCGGTCGTTTTTCACGATGAATTTTGACCGCACTTTTCTTTATCATTTTTCGCTATGCAATTAGAACCCGATTTTTGGCTACACAAAACCTTACTTGACATGAACGATGCCGAGTGGGAGGCGTTATGTGACGGGTGTGGGAAATGTTGCTATCGTAAATATATTGACGGCTATGGCAAATATGAACATTTGTATTACACGCGCATTGCCTGCAATCTGTTGGAGTTGGAAACGGGAAAGTGCGGTCAATATTCACGGCGTTTTGAATTGGAAGAAGACTGCACCAAGCTCACCAAAGAAAACTTGTCGGATTTCGATTGGTTACCCGCCACCTGTGCTTATCGTTTATTACATGAAGGAAAACCGTTGCCCTCATGGCATCCGCTTATCAGCGGCGATCCCGATAGCGTGAAAAAAGCCGATATT  >  1/1040760‑1041243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑