Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,033,915	C→T	I329I (ATC→ATT)	unknown →	Esterase/lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,033,915	0	C	T	100.0%	146.4 / NA	54	I329I (ATC→ATT)	unknown	Esterase/lipase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

ATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGTGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCATTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATCGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAGTCAGAGCTTGTCGAGCTTCTGGCTATCAAAA  >  1/1033675‑1034161                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       aTCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGt                                                                                                                                                                                                                                              <  1:45715/251‑1 (MQ=255)      cACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                                                                         <  1:387728/251‑1 (MQ=255)       aCCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCta                                                                                                                                                                                                                                        <  1:95481/251‑1 (MQ=255)           gCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATaaa                                                                                                                                                                                                                                    >  1:145930/1‑251 (MQ=255)            cATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATaaaa                                                                                                                                                                                                                                   >  1:744244/1‑251 (MQ=255)                                      ggCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGGAAGCaccgt                                                                                                                                                                                                           >  1:860908/1‑245 (MQ=255)                                            aataatTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGa                                                                                                                                                                                                   <  1:432274/251‑1 (MQ=255)                                              taatTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGaaa                                                                                                                                                                                                 <  1:512607/251‑1 (MQ=255)                                                         gTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATgggg                                                                                                                                                                                       <  1:861637/250‑1 (MQ=255)                                                              gAAAGCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGGACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATGCTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGc                                                                                                                                                                                  <  1:395143/250‑1 (MQ=255)                                                                  ccGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                                                                    >  1:779200/1‑180 (MQ=255)                                                                     tGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGc                                                                                                                                                                           <  1:856688/250‑1 (MQ=255)                                                                          aatCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATgggg                                                                                                                                                                                       <  1:252296/233‑1 (MQ=255)                                                                          aatCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGa                                                                                                                                                                     <  1:35027/251‑1 (MQ=255)                                                                               gCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                                                                         >  1:147507/1‑178 (MQ=255)                                                                               gCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGa                                                                                                                                                                                                   <  1:347831/216‑1 (MQ=255)                                                                                       ccGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGAt                                                                                                                                                        <  1:886790/251‑1 (MQ=255)                                                                                                     cAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                                                                    >  1:679117/1‑145 (MQ=255)                                                                                                       acacAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGc                                                                                                                                                                                              <  1:84188/197‑1 (MQ=255)                                                                                                            gCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGc                                                                                                                                                                                                                        <  1:417289/166‑1 (MQ=255)                                                                                                            gCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGAAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGGTTCAGTGGGTGGAt                                                                                                                                   >  1:261573/1‑251 (MQ=255)                                                                                                              aCGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAg                                                                                                                                 <  1:111146/251‑1 (MQ=255)                                                                                                                 gAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                                                                         >  1:787782/1‑144 (MQ=255)                                                                                                                      gggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                                                                    <  1:854424/128‑1 (MQ=255)                                                                                                                       ggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                                                                         <  1:87170/250‑1 (MQ=255)                                                                                                                       ggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                                                                         <  1:732043/250‑1 (MQ=255)                                                                                                                                              ggtgCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGGTGTTTCAgtg                                                                                                                                          >  1:479642/1‑210 (MQ=255)                                                                                                                                                 gcgCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGc                                                                                                                          <  1:175379/221‑1 (MQ=255)                                                                                                                                                    cTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGGTTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCggg                                                                                           >  1:34307/1‑250 (MQ=255)                                                                                                                                                               ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTg                                                                                                                                                                                                                                <  1:655911/107‑1 (MQ=255)                                                                                                                                                               ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGGTAGTATTAGCAAATAGTTTACGGGAATGGGAAAAGTGCGGTGGGGTTTTACa                                                                                >  1:291716/1‑251 (MQ=255)                                                                                                                                                               ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                                                                         <  1:160883/210‑1 (MQ=255)                                                                                                                                                                   gAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATaaa                                                                            >  1:458602/1‑251 (MQ=255)                                                                                                                                                                   gAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGATAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTCTGAAACGCCCTGGGGGGTGCCGCATGCGGGGGTTTATGTTTTGGATGAGTTGTTTCAGAGGGTGGATAGTATTAGCCAATAGTTTACGGGAATGGTAAAAGTGCGGTGGGGTTTTaatattg                                                                            >  1:409155/1‑245 (MQ=255)                                                                                                                                                                             gATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGt                                                                                                                                                                                                                                              <  1:587439/79‑1 (MQ=255)                                                                                                                                                                             gATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGGTTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGGATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGGGGGGGTTTACATAAAGCATCGGca                                                                   >  1:80868/1‑250 (MQ=255)                                                                                                                                                                                  aaGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGcactcact                                                             <  1:582948/251‑1 (MQ=255)                                                                                                                                                                                  aaGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGGGTTTTACATAAAGCATCGGcactca                                                               >  1:353763/1‑249 (MQ=255)                                                                                                                                                                                    gTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCa                                                                                                                         >  1:900084/1‑189 (MQ=255)                                                                                                                                                                                       aaaCGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATGGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGt                                                        <  1:436060/251‑1 (MQ=255)                                                                                                                                                                                         aCGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTag                                                      <  1:302714/251‑1 (MQ=255)                                                                                                                                                                                            cagcaTTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGGTTATGATTTGGATGAGTTGTTTCAGGGGGTGGGTAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGGGGTGGTTTACATAAAGCATCGGCACTCACTACTGTAGAgg                                                   >  1:375680/1‑251 (MQ=255)                                                                                                                                                                                              gcaTTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTg                                                                                               >  1:76388/1‑205 (MQ=255)                                                                                                                                                                                                    gcgcACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTAt                                                                                                                              <  1:203275/168‑1 (MQ=255)                                                                                                                                                                                                           cGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAg                                       >  1:739833/1‑248 (MQ=255)                                                                                                                                                                                                                              gTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAGTCAGAGCTTGTGGAg                  >  1:798733/1‑250 (MQ=255)                                                                                                                                                                                                                                    cTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGGGTTTTACATAAAGCATCGGCACTCACTACTGt                                                        >  1:552961/1‑206 (MQ=255)                                                                                                                                                                                                                                           cGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGGAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGGTTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGGTTACGGGAATGGTAAAAGTGCGGGGGTGGTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAGTCAGAGCTTGTGGGGCTTCTGGCTATCaa    >  1:346382/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCt                                     <  1:901512/217‑1 (MQ=255)                                                                                                                                                                                                                                             cTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTTAAAAGGAGGATTATGAAACCCCATGGGGCGTGCCGCCCGCGGGGGATTCTGATTTGGGTGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACggg                                                                                                            >  1:780651/1‑145 (MQ=255)                                                                                                                                                                                                                                             cTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGGTTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCAAAAAGTTTACGGGAATGGTAAAAGTGCGGGGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAg                                       >  1:624229/1‑214 (MQ=255)                                                                                                                                                                                                                                             cTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTaa                                                                                                    <  1:804225/153‑1 (MQ=255)                                                                                                                                                                                                                                             cTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAg                                 <  1:461362/220‑1 (MQ=255)                                                                                                                                                                                                                                             cTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAGTCAGAGCTTGTGGAGCTTCTGGCTATCaaaa  >  1:477184/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       ATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGTGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCATTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATCGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGGACGATCGTTGAGCTTGAGTCAGAGCTTGTCGAGCTTCTGGCTATCAAAA  >  1/1033675‑1034161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑