Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,033,875	T→C	I316T (ATT→ACT)	unknown →	Esterase/lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,033,875	0	T	C	100.0%	133.2 / NA	50	I316T (ATT→ACT)	unknown	Esterase/lipase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/29);  total (21/29)

GGACGGTTATATTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGTGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCATTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATCGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGG  >  1/1033642‑1034112                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              ggACGGTTATATTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCg                                                                                                                                                                                                                              >  1:127297/1‑251 (MQ=255)      gTTATATTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGt                                                                                                                                                                                                                         >  1:719169/1‑251 (MQ=255)      gTTATATTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGt                                                                                                                                                                                                                         >  1:483800/1‑251 (MQ=255)           atTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATAc                                                                                                                                                                                                                    <  1:21785/251‑1 (MQ=255)           atTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGCTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATAc                                                                                                                                                                                                                    <  1:720349/251‑1 (MQ=255)             tCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACtt                                                                                                                                                                                                                  <  1:680738/251‑1 (MQ=255)                                  aTCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGt                                                                                                                                                                                             <  1:45715/251‑1 (MQ=255)                                       cACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                        <  1:387728/251‑1 (MQ=255)                                        aCCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCta                                                                                                                                                                                       <  1:95481/251‑1 (MQ=255)                                            gCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATaaa                                                                                                                                                                                   >  1:145930/1‑251 (MQ=255)                                             cATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATaaaa                                                                                                                                                                                  >  1:744244/1‑251 (MQ=255)                                                                       ggCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGGAAGCaccgt                                                                                                                                                          >  1:860908/1‑245 (MQ=255)                                                                             aataatTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGa                                                                                                                                                  <  1:432274/251‑1 (MQ=255)                                                                               taatTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGaaa                                                                                                                                                <  1:512607/251‑1 (MQ=255)                                                                                          gTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATgggg                                                                                                                                      <  1:861637/250‑1 (MQ=255)                                                                                               gAAAGCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGGACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATGCTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGc                                                                                                                                 <  1:395143/250‑1 (MQ=255)                                                                                                   ccGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                   >  1:779200/1‑180 (MQ=255)                                                                                                      tGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGc                                                                                                                          <  1:856688/250‑1 (MQ=255)                                                                                                           aatCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATgggg                                                                                                                                      <  1:252296/233‑1 (MQ=255)                                                                                                           aatCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGa                                                                                                                    <  1:35027/251‑1 (MQ=255)                                                                                                                gCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                        >  1:147507/1‑178 (MQ=255)                                                                                                                gCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGa                                                                                                                                                  <  1:347831/216‑1 (MQ=255)                                                                                                                        ccGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGAt                                                                                                       <  1:886790/251‑1 (MQ=255)                                                                                                                                      cAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                   >  1:679117/1‑145 (MQ=255)                                                                                                                                        acacAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGc                                                                                                                                             <  1:84188/197‑1 (MQ=255)                                                                                                                                             gCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGc                                                                                                                                                                       <  1:417289/166‑1 (MQ=255)                                                                                                                                             gCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGAAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGGTTCAGTGGGTGGAt                                                                                  >  1:261573/1‑251 (MQ=255)                                                                                                                                               aCGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAg                                                                                <  1:111146/251‑1 (MQ=255)                                                                                                                                                  gAAAAGGGAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCt                                                                                                                                                                                        >  1:787782/1‑144 (MQ=255)                                                                                                                                                       gggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTg                                                                                                                                                                                                   <  1:854424/128‑1 (MQ=255)                                                                                                                                                        ggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                        <  1:732043/250‑1 (MQ=255)                                                                                                                                                        ggAAAGATGGCGGATAAAAATGTGGTGCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                        <  1:87170/250‑1 (MQ=255)                                                                                                                                                                               ggtgCGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGGTGTTTCAgtg                                                                                         >  1:479642/1‑210 (MQ=255)                                                                                                                                                                                  gcgCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGc                                                                         <  1:175379/221‑1 (MQ=255)                                                                                                                                                                                     cTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGGTTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCggg                                          >  1:34307/1‑250 (MQ=255)                                                                                                                                                                                                ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTg                                                                                                                                                                               <  1:655911/107‑1 (MQ=255)                                                                                                                                                                                                ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGGTAGTATTAGCAAATAGTTTACGGGAATGGGAAAAGTGCGGTGGGGTTTTACa                               >  1:291716/1‑251 (MQ=255)                                                                                                                                                                                                ccATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCa                                                                        <  1:160883/210‑1 (MQ=255)                                                                                                                                                                                                    gAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATaaa                           >  1:458602/1‑251 (MQ=255)                                                                                                                                                                                                    gAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGATAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGATTCTGAAACGCCCTGGGGGGTGCCGCATGCGGGGGTTTATGTTTTGGATGAGTTGTTTCAGAGGGTGGATAGTATTAGCCAATAGTTTACGGGAATGGTAAAAGTGCGGTGGGGTTTTaatattg                           >  1:409155/1‑245 (MQ=255)                                                                                                                                                                                                              gATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGt                                                                                                                                                                                             <  1:587439/79‑1 (MQ=255)                                                                                                                                                                                                              gATCAAAGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGGGGGTTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGGATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGGGGGGGTTTACATAAAGCATCGGca                  >  1:80868/1‑250 (MQ=255)                                                                                                                                                                                                                   aaGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGcactcact            <  1:582948/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGGGTTTTACATAAAGCATCGGcactca              >  1:353763/1‑249 (MQ=255)                                                                                                                                                                                                                     gTAAAACGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGGGGATAGTATTAGCa                                                                        >  1:900084/1‑189 (MQ=255)                                                                                                                                                                                                                        aaaCGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATGGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGt       <  1:436060/251‑1 (MQ=255)                                                                                                                                                                                                                          aCGCAGCATTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTag     <  1:302714/251‑1 (MQ=255)                                                                                                                                                                                                                             cagcaTTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGGTTATGATTTGGATGAGTTGTTTCAGGGGGTGGGTAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGGGGTGGTTTACATAAAGCATCGGCACTCACTACTGTAGAgg  >  1:375680/1‑251 (MQ=255)                                                                                                                                                                                                                               gcaTTGGCGCACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTg                                              >  1:76388/1‑205 (MQ=255)                                                                                                                                                                                                                                     gcgcACTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATTGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTAt                                                                             <  1:203275/168‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              GGACGGTTATATTCACGCGGAAAAACGAATGAAATCGCCACCGGCATTTGATGCGCTGAATTTGAGTTCGGGCGAAAATAATTTATTTGGTACGGAAACCGTGAATAATCAGCATTTCACCGTTTTTTCTTGGCAACACAGCACGGAAAAGGGAAAGATGGCGGATAAAAATGTGGTGTGCTTAATGAACGCCATGAATTATGTGGATCAAAGTAAAACGCAGCATTGGCGCATTCGGGTCGGCACCTCCGACCGTGATACTTCGCACGCTATCGGTGCTATGTTAGCTATAAAATTGCGTATGGCGGGTAAGCAGGTGGATTATGAAACGCCATGGGGCGTGCCGCATGCGGGGGATTATGATTTGGATGAGTTGTTTCAGTGGGTGGATAGTATTAGCAAATAGTTTACGGGAATGGTAAAAGTGCGGTGGTGTTTTACATAAAGCATCGGCACTCACTACTGTAGAGG  >  1/1033642‑1034112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑