Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,032,618	A→C	intergenic (‑27/‑51)	unknown ← / → unknown	hypothetical protein/Esterase/lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,032,618	0	A	C	100.0%	94.6 / NA	35	intergenic (‑27/‑51)	unknown/unknown	hypothetical protein/Esterase/lipase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/15);  total (20/15)

CGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATACGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATAGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGATCCGTTGGCGGAGGCACCTTATTTTAATAAAACGGGTAACGGCTATGATTTGCAATTCTCGCCGAAAACCTACCGCACTTTGGAGGCGAAGGTGAACGGGGAAA  >  1/1032388‑1032855                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              cgcCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                                                                                                                                                           <  1:641532/251‑1 (MQ=255)        gTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGCTCGGTGTTTTACATCCATAAAATGAAAATAATATGAAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGTGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGta                                                                                                                                                                                                                    >  1:832206/1‑251 (MQ=255)              aTTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                                                                                                                                                              <  1:730142/251‑1 (MQ=255)              aTTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                                                                                                                                                              <  1:809627/251‑1 (MQ=255)                 ggTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAg                                                                                                                                                                                                                      >  1:312579/1‑240 (MQ=255)                 ggTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCtt                                                                                                                                                                                                              <  1:768109/248‑1 (MQ=255)                       gTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAgg                                                                                                                                                                                                     >  1:664625/1‑251 (MQ=255)                                           aTTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                                                                                                                                                           >  1:139164/1‑209 (MQ=255)                                                  aTTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAAc                                                                                                                                                                                                                        <  1:900721/205‑1 (MQ=255)                                                       cccTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCt                                                                                                                                                                                                                                    <  1:715202/188‑1 (MQ=255)                                                            gTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGAc                                                                                                                                                                                                                                            <  1:222845/175‑1 (MQ=255)                                                                                 tGTTCGGTGTTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATgg                                                                                                                                                                           <  1:764738/219‑1 (MQ=255)                                                                                         gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATc                                                                                                                                                                                                                           >  1:707265/1‑163 (MQ=255)                                                                                         gtTTTACATCCATAAAATGAAAATAATATGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGgtgt                                                                                                                                   >  1:147877/1‑251 (MQ=255)                                                                                                                                        aGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttat                                                                                                   >  1:352115‑M1/1‑228 (MQ=255)                                                                                                                                          ttACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGc                                                                                                                                                                                         <  1:234226/148‑1 (MQ=255)                                                                                                                                                        gATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttg                                                                     >  1:568517‑M1/1‑212 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATgg                                                                                                                                                                           <  1:112045/147‑1 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTtggtgg                                                                                                                                                           >  1:827749/1‑163 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgca                                                                   >  1:533493‑M1/1‑211 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgca                                                                   >  1:48318‑M1/1‑211 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgca                                                                   >  1:405638‑M1/1‑211 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatga                                                                         >  1:688096‑M1/1‑211 (MQ=255)                                                                                                                                                         atatTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatga                                                                         >  1:335341‑M1/1‑211 (MQ=255)                                                                                                                                                                      gTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATc                                                                                                                                                                                                                                <  1:474431/81‑1 (MQ=255)                                                                                                                                                                      gTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcacctta                                                                                                    >  1:235887‑M1/1‑198 (MQ=255)                                                                                                                                                                                       gTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAAc                                                                                                                                                                                                                        <  1:62965/72‑1 (MQ=255)                                                                                                                                                                                             tGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgcaattctcgccgaaaacctaccgcactttggaggcgaa                               <  1:722309‑M1/251‑77 (MQ=255)                                                                                                                                                                                                          cACTTTTTTGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgcaattctcgccgaaaacctaccgcactttggaggcgaaggtgaacggggaa                  >  1:168986‑M1/1‑162 (MQ=255)                                                                                                                                                                                                               ttttGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGcacac                                                                                                                                                                                     >  1:700908/1‑83 (MQ=255)                                                                                                                                                                                                                 ttGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATc                                                                                                                                                                                                                >  1:515466/1‑54 (MQ=255)                                                                                                                                                                                                                 ttGTTACACCTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgcaattctcgccgaaaacctaccgcactttggaggcgaaggt                            >  1:124049‑M1/1‑155 (MQ=255)                                                                                                                                                                                                                          cTACCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGcac                                                                                                                                                                                       <  1:405246/70‑1 (MQ=255)                                                                                                                                                                                                                            aCCGAACTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgcaattctcgccgaaaacctaccgcactttggaggcgaaggtgaacggggaaaccgtgaaattccgtg  >  1:283347‑M1/1‑144 (MQ=255)                                                                                                                                                                                                                                aaCTCATCGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGcaccttattttaataaaacgggtaacggctatgatttgcaattctcgccgaaaacctaccgcactttggaggcgaaggtgaacggggaaac                <  1:758760‑M1/231‑92 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              CGCCCTTGTTTTCATTGGTTTTGTTCCACTTATTCCCGCTTGATTCAACATTCTCCCTAGTTTATTCCTTTTTCTCATTTTGTTCGGTGTTTTACATCCATAAAATGAAAATAATACGTAAATTTTAGTAAAACGAGTTACATTTTCAAGTGATATTGACTTGATGTTTATATGATTTTTAAGTGCGGTGAAAATCAACCGCACTTTTTTGTTACACCTACCGAACTCATAGACGATACGCTCATCTAATCAACAGTATATCTTTTAAACAGGAAAATCTTATGCACACAAAAACATGGTTGGCTTCTTTGGTGGGGTTGGTGATTTCAGGAACGGTGTGGGCGAATCCGCCGCAGGCGCAGGATCCGTTGGCGGAGGCACCTTATTTTAATAAAACGGGTAACGGCTATGATTTGCAATTCTCGCCGAAAACCTACCGCACTTTGGAGGCGAAGGTGAACGGGGAAA  >  1/1032388‑1032855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑